Evaluation of various packings for solid-state catalytic reactors used in the liquid chromatographic detection of non-reducing carbohydrates

Comparative study of post-column reactions for the detection of saccharides in liquid chromatography

“…Straight lines going through zero were obtained for all four saccharides tested up to 1-Mg quantities (for absorbance values, see Table II). Under these experimental conditions detection limits were as follows: raffinose, 28 ng; sucrose, 15 ng; glucose, 21 ng; and fructose, 19 ng at a noise level of 1.6 X 1CP3 absorbance units and a 3:1 signal-to-noise ratio.…”

“…Another detection reagertt for nonreducing sugars has also been reported recently (18) but its detection wavelength of 260 nm makes it more prone to interferences (see Figure 5 below). To adapt these detection reagents also to some nonreducing oligosaccharides, Vrátny et al (19) suggested use of a solid phase catalytic reactor, which converts them to easily detectable reducing subunits. This catalytic hydrolysis technique has been optimized recently (20).…”

Ligand-exchange indirect conductometric detection of electrically neutral carbohydrates and alcohols after high-performance liquid chromatographic separation

“…Carbohydrates and alcohols are required to be measured sensitively in many fields, because they are important both chemically and biologically. As a result of fundamental and applicable investigations, many workers have noticed that high-performance liquid chromatography (HPLC) is a useful tool for measuring these compounds and have published many papers dealing with HPLC separation and detection (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12).…”

“…Therefore, the other direction of the investigation, that is, developing universal detection, is required. Vrátny et al reported the use of a catalyst that can hydrolyze polysaccharides to detectable reducing sugars (4,11). Grimble et al demonstrated the postcolumn reaction method with cuprammonium as a reaction reagent.…”

“…Amines are usually determined by gas chromatography (7)(8)(9) or liquid chromatography (10,11). Both of these techniques usually require some sample cleanup of complicated matrices like urine.…”