Evaluation of ventricular and arterial hemodynamics in anesthetized closed-chest mice

Fentzke, Richard C.; Korcarz, Claudia E.; Shroff, Sanjeev G.; Lin, Hua; Sandelski, Joanne; Leiden, Jeffrey M.; Lang, Roberto M.

doi:10.1016/s0894-7317(97)80008-9

Cited by 39 publications

(28 citation statements)

References 20 publications

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“…Transthoracic 2D echocardiography has been employed extensively to discern structural and functional differences among genetically engineered mice. In our laboratory, transgenic mice expressing a dominant-negative form of the CREB transcription factor (CREB A133 ) were produced to develop a dilated cardiomyopathy (DCM) that closely resembles the anatomical, physiological, and clinical features of the human disease (7)(8)(9). Serial echocardiography was employed to evaluate the progression of the phenotype.…”

Section: Chamber Dimensions and Wall Thicknessesmentioning

confidence: 99%

“…More recently, Doppler ultrasound techniques, which allow the noninvasive assessment of cardiac output multiple times per experiment and the ability to follow changes serially, have been used. Stroke volume and ascending aortic blood velocity can be calculated from continuous wave aortic Doppler velocity recordings, acquired from the parasternal long-axis view using 12-to 15-MHz transducers and 2D-targeted M-mode echocardiographic measurements of the diameter of the proximal ascending aorta (9). Other investigators (3,43) have used different approaches, recording peak aortic velocities from the suprasternal approach or the apical window.…”

Section: Ejection Phase Indices Including Cardiac Outputmentioning

confidence: 99%

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Use of echocardiography for the phenotypic assessment of genetically altered mice

Collins

Korcarz

Lang

2003

Physiological Genomics

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125

106

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Transgenic mice displaying abnormalities in cardiac development and function represent a powerful new tool for understanding molecular mechanisms underlying normal cardiovascular function and the pathophysiological bases of human cardiovascular disease. Complete cardiac evaluation of phenotypic changes in mice requires the ability to noninvasively assess cardiovascular structure and function in a serial manner. However, the small mouse heart beating at rates in excess of 500 beats/min presents unique methodological challenges. Two-dimensional and Doppler echocardiography have been recently used as effective, noninvasive tools for murine imaging, because quality images of cardiac structures and valvular flows can be obtained with newer high-frequency transthoracic transducers. We will discuss the use of echocardiography for the assessment of 1) left ventricular (LV) chamber dimensions and wall thicknesses, 2) LV mass, 3) improved endocardial border delineation using contrast echocardiography, 4) LV contractility using ejection phase indices and load-independent indices, 5) vascular properties, and 6) LV diastolic performance. Evaluation of cardiovascular performance in closed chest mice is feasible in a variety of murine models using Doppler echocardiographic imaging. Doppler echocardiography; left ventricular performance GENETIC MODIFICATIONS are frequently used to produce mice models that are used to investigate the molecular basis of cardiac growth and development (7-9, 18, 24, 25) These technologies have led to a proliferation of transgenic and knockout mice models displaying a variety of cardiovascular phenotypes. To evaluate these phenotypes, it is necessary to develop accurate, reproducible, and noninvasive methods to assess cardiac morphology and function in a serial manner.Although it is currently possible to assess cardiac hemodynamics and function using ventricular catheterization, radiolabeled microspheres, and thermodilution techniques in mice, these methods require invasive instrumentation, which restrict the ability to measure physiological changes in a serial manner. Ultrasound imaging has been increasingly applied to identify and characterize structural and functional features of different cardiac phenotypes and pathophysiological responses to surgical and pharmacological interventions in large animal models of human disease (7-9, 16, 17, 27, 43). The small size of the mouse heart, which beats at heart rates in excess of 500 beats/min, presents unique methodological challenges for cardiac ultrasound. Recently developed broadband, phased-array, and linear transducers have small footprints, which are capable of both high frame-rate imaging and improved nearfield imaging, thereby generating high-quality images of the mouse heart.Imaging of the small, fast-beating murine hearts also requires special technical attention to the selection of proper anesthesia. In addition to anesthetic effects, in this review, we will discuss the preparation for image acquisition using currently available echocardiogr...

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Section: Chamber Dimensions and Wall Thicknessesmentioning

confidence: 99%

Section: Ejection Phase Indices Including Cardiac Outputmentioning

confidence: 99%

Use of echocardiography for the phenotypic assessment of genetically altered mice

Collins

Korcarz

Lang

2003

Physiological Genomics

Self Cite

125

106

View full text Add to dashboard Cite

show abstract

“…Recent technological advances have made possible the echocardiographic assessment of murine cardiac structure and function (37)(38)(39)(40)(41). Accordingly, we assessed both the LV dimensions and systolic function of the CREB A133 mice using two-dimensionally targeted M-mode echocardiography acquired both at baseline and following stimulation with the ␤-adrenergic agonist, isoproterenol (Fig.…”

Section: Production and Molecular Characterization Of Creb A133 Transmentioning

confidence: 99%

Dilated cardiomyopathy in transgenic mice expressing a dominant-negative CREB transcription factor in the heart.

Fentzke¹,

Korcarz²,

Lang³

et al. 1998

J. Clin. Invest.

228

192

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Idiopathic-dilated cardiomyopathy (IDC) is a common primary myocardial disease of unknown etiology characterized by progressive biventricular failure, cardiac dilatation, and premature mortality. Here we show that transgenic mice expressing a dominant-negative form of the CREB transcription factor (CREB A133 ) under the control of the cardiac myocyte-specific ␣ -MHC promoter develop dilated cardiomyopathy that closely resembles many of the anatomical, physiological, and clinical features of human IDC. Between 2 and 20 wk of age, these mice develop four chamber cardiac dilatation, decreased systolic and diastolic left ventricular function, and attenuated contractile responses to the ␤ -adrenergic agonist, isoproterenol. Histologically, the CREB A133 hearts demonstrated both atrophic and hypertrophied fibers as well as significant interstitial fibrosis. These anatomical and hemodynamic changes were associated with hepatic congestion and peripheral edema, intracardiac thrombi, and premature mortality. Taken together, these results implicate CREB as an important regulator of cardiac myocyte function and provide a genetic model of dilated cardiomyopathy which should facilitate studies of both the pathogenesis and therapy of this clinically important disorder. (

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“…[1][2][3][4][5][6][7][8][9] However, M-mode method has some potential problems when used for mice. It is well known that the M-mode method has limited reliability in estimating global function in an infarcted human heart 10 and that 2-D echocardiography is more suitable.…”

Section: Echocardiographic Assessment Of Global Systolic Function In mentioning

confidence: 99%

Assessment of Left Ventricular Systolic Function Using Contrast Two-Dimensional Echocardiography With a High-Frequency Transducer in the Awake Murine Model of Myocardial Infarction.

Suehiro

Takuma²,

Shimizu³

et al. 2001

Jpn Circ J

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eft coronary artery ligation is one of the most common methods of creating a model of myocardial infarction (MI) in the murine heart and M-mode echocardiography has been used to assess cardiac function because of its higher sampling frequency, which is particularly useful when assessing a heart rate (HR) of 600 beats/min or greater. 1-9 However, the estimation of global left ventricular (LV) function using M-mode echocardiography has limitations when it is applied to an infarcted heart 10 and 2-dimensional (2-D) echocardiography is more suitable. In murine hearts with their small size and high heart rate, however, even this method has been limited in its application for assessment of cardiac function because of the difficulty in obtaining adequate 2-D images.The recent improvements in 2-D echocardiography of a higher resolution and higher frame rate using a highfrequency transducer provide more accessible images for

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Evaluation of ventricular and arterial hemodynamics in anesthetized closed-chest mice

Cited by 39 publications

References 20 publications

Use of echocardiography for the phenotypic assessment of genetically altered mice

Use of echocardiography for the phenotypic assessment of genetically altered mice

Dilated cardiomyopathy in transgenic mice expressing a dominant-negative CREB transcription factor in the heart.

Assessment of Left Ventricular Systolic Function Using Contrast Two-Dimensional Echocardiography With a High-Frequency Transducer in the Awake Murine Model of Myocardial Infarction.

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