Evaluation of volumes of Ceratitis capitata larvae in exposure devices to Diachasmimorpha longicaudata in mass rearing conditions in Costa Rica

Abstract

Five volumes (60 cc, 70 cc, 80 cc, 90 cc and 100 cc) of L 3 of Ceratitis capitata in exposure devices to the parasitoid Diachasmimorpha longicaudata were evaluated in mass rearing conditions with the objective of finding the optimal quantity of host larvae to obtain the best parasite quality parameters. The variables like parasitism, sexual proportion and fertility were analyzed with ANOVA and compared with Tukey's range test. Significant differences were found for treatment in the variable parasitism (ρ = 0.0244), showing that the treatments of 60 cc and 70 cc obtained the highest number of parasitized larvae. The variables like sex ratio and fertility did not show any difference between treatments, but for fecundity there were variations for days 5 and 3 (ρ<0.0001). The flight ability, male and female mortality and fertility variables were analyzed with the model of logistic regression analysis with autocorrelation correction (response variable is binomial), for which treatment effects were found only in the variable mortality of males for the treatment 70 cc. According to these results, the volume of 70 cc would be the promissory treatment for industrial production in order to obtain greater parasitism and a longer life of males.

Dietas Artificiais para a Criação de Ceratitis capitata (Wiedemann 1824) (Diptera: Tephritidae)

“…Management strategies for the effective control of this pest insect have become a global need, driving several studies on this problem. Sterile insect technique (Nikolouli et al, 2020), mass rearing of parasitoids (Morera-Montoya et al, 2019), experimentation with the use of entomopathogenic microorganisms (Gava et al, 2021) and plant extracts (Benelli et al, 2021) are some of the strategies with potential for the control of C. capitata in the field and that require testing steps in the laboratory with the need for mass rearing of this pest insect. The success of these techniques depends on obtaining welldeveloped insects, which is directly related to the use of suitable artificial diets (Aceituno-Medina, 2019).…”