Evaluation of wastewater treatment in a novel anoxic–aerobic algal–bacterial photobioreactor with biomass recycling through carbon and nitrogen mass balances

Alcántara, Cynthia; Domínguez, J. M.; García, Dimas; Blanco, Saúl; Pérez, Rebeca; García‐Encina, Pedro A.; Muñoz, Raúl

doi:10.1016/j.biortech.2015.04.125

Cited by 105 publications

(39 citation statements)

References 28 publications

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“…This mass balance approach allowed to better understand the symbiosis between microalgae and bacteria in this novel anoxic-aerobic algal-bacterial photobioreactor configuration [14], by quantifying the extent of the mechanisms involved in C, N and P removal in each reactor.…”

Section: Resultsmentioning

confidence: 99%

“…These short HRTs are required to make algal-bacterial processes competitive with activated sludge systems. The experimental design was based on the operational limitations identified in previous studies in this AA-ABPh configuration [13] [14]. The experiment was divided in three operational stages.…”

Section: Experimental Designmentioning

confidence: 99%

“…The pH in the photobioreactor was maintained between 7.0 and 8.6 during SI by daily addition of 1.2 mL of chlorhydric acid (37%), while biogas upgrading into the absorption column supplied CO 2 to overcome the IC limitation encountered during SI and to maintain the pH below inhibitory values for bacterial activity (< 9) during SII and SIII. The synthetic biogas mixture supplied was composed of methane (70%), evaluate the richness and composition of the bacterial communities [14]. Finally, the composition of the biogas at the inlet and outlet of the absorption column was determined twice a week during SII and SIII.…”

Section: Experimental Designmentioning

confidence: 99%

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Enhanced carbon, nitrogen and phosphorus removal from domestic wastewater in a novel anoxic-aerobic photobioreactor coupled with biogas upgrading

García

Alcántara

Blanco³

et al. 2017

Chemical Engineering Journal

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Section: Resultsmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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Enhanced carbon, nitrogen and phosphorus removal from domestic wastewater in a novel anoxic-aerobic photobioreactor coupled with biogas upgrading

García

Alcántara

Blanco³

et al. 2017

Chemical Engineering Journal

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“…Besides ROS quenching, several studies have reported that heterotrophic partner can provide the necessary inorganic carbon by decomposing organic matter or growth factor such as vitamin B 12 to cyanobacteria in the natural and artificial consortium [18,59,60], which we will also analyze in the future work. Further analysis of interaction in the consortium could provide the necessary theoretical basis for potential application of artificial co-culture systems in many areas, such as controlling blooms [61], degradation of pollutant [62][63][64], and soil remediation [65][66][67].…”

Section: Discussionmentioning

confidence: 99%

Construction and analysis of an artificial consortium based on fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce platform chemical 3-hydroxypropionic acid from CO2

Zhang

Chen

Diao

et al. 2020

Preprint

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Background: Cyanobacterial carbohydrates, such as sucrose, have been considered as a potential renewable feedstock to support the production of fuels and chemicals. However, the separation and purification processes of these carbohydrates will increase the production cost of chemicals. It has been proposed that co-culture fermentation is an efficient and economical way to utilize these cyanobacterial carbohydrates. However, studies about the application of co-culture system to achieve the green biosynthesis of platform chemicals are still rare.Results: In this study, we successfully achieved one-step conversion of sucrose derived from cyanobacteria to fine chemicals by constructing a microbial consortium consisted of fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 and Escherichia coli to sequentially produce sucrose and then platform chemical 3-hydroxypropionic acid (3-HP) from CO2 under photoautotrophic growth condition. First, efforts were made to overexpress sucrose permease coding gene cscB under strong promoter Pcpc560 in S. elongatus UTEX 2973 for efficient sucrose secretion. Second, sucrose catabolic pathway and malonyl CoA-dependent 3-HP biosynthetic pathway were introduced into E. coli BL21(DE3) for heterologous biosynthesis of 3-HP from sucrose. By optimizing the cultivation temperature from 37℃ to 30℃, a stable artificial consortium system was constructed with the capability of producing 3-HP up to 68.29 mg/L directly from CO2. In addition, cell growth of S. elongatus UTEX 2973 in the consortium was enhanced, probably due to the quick quenching of reactive oxygen species (ROS) in the system by E. coli, which in turn improved photosynthesis of cyanobacteria. Conclusion: The study demonstrated the feasibility of achieving the one-step conversion of sucrose to fine chemicals using an artificial consortium system. The study also confirmed that the heterotrophic bacteria could promote cell growth of cyanobacteria by relieving oxidative stress in this microbial consortium, which further suggests the potential values of this system for the future industrial application.

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“…Cyanobacteria vary in preference for light intensity, temperature, nutrient concentrations, salinity and pH, as well as in capabilities to tolerate different ammonia, heavy metals and other toxic substances concentrations [7]. Many studies exist on municipal wastewater phycoremediation [8,9] and some authors explore the growth of cyanobacteria on swine [10] or poultry litter effluent as well as the paper industry [11] or carpet industry [12]. However, the viability of cyanobacteria in industrial wastewater is still a challenge.…”

Section: Introductionmentioning

confidence: 99%

Growth Aspects and Biochemical Composition of Synechococcus sp. MK568070 Cultured in Oil Refinery Wastewater

Blažina

Haberle

Hrustić

et al. 2019

JMSE

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The aim of the study was to explore the possibility of bioremediation of oil refinery wastewaters by the cyanobacterium Synechococcus sp. MK568070, isolated from the Adriatic Sea. The potential of biomass and lipid production was explored upon cultivation on oil refinery wastewater with excess CO2 after the removal of nutrients. The strain grew well in a wide range of salinities and ammonium concentrations, and was further tested on the wastewater from local oil refinery plant of various N-composition. Growth experiment under optimized conditions was used to analyze the lipid, carbohydrate and protein dynamics. The biomass yield was highly dependent on nutrient source and concentration, salinity and CO2 addition. Highest biomass yield was 767 mg/L of dry weight. Towards the end of the experiment the decline in carbohydrate to 18.9% is visible, whereas at the same point lipids, in particular saturated fatty acid methyl esters (FAME), started to accumulate within the cells. The content of lipids at the end of the experiment was 21.4%, with the unsaturation index 0.45 providing good biofuel feedstock characteristics. Fourier Transform Infrared (FTIR) spectroscopy analysis demonstrated a high degree of lipid accumulation in respect to proteins, along with the structural changes and biomass accumulation. In addition, the N-removal from the wastewater was >99% efficient. The potential of lipid accumulation, due to the functional photosynthesis even at the minimal cell quota of nutrients, is critical for the usage of excess industrial CO2 and its industrial transformation to biodiesel. These findings enable further considerations of Synechococcus sp. (MK568070) for the industrial scale biomass production and wastewater remediation.

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Evaluation of wastewater treatment in a novel anoxic–aerobic algal–bacterial photobioreactor with biomass recycling through carbon and nitrogen mass balances

Cited by 105 publications

References 28 publications

Enhanced carbon, nitrogen and phosphorus removal from domestic wastewater in a novel anoxic-aerobic photobioreactor coupled with biogas upgrading

Enhanced carbon, nitrogen and phosphorus removal from domestic wastewater in a novel anoxic-aerobic photobioreactor coupled with biogas upgrading

Construction and analysis of an artificial consortium based on fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce platform chemical 3-hydroxypropionic acid from CO2

Growth Aspects and Biochemical Composition of Synechococcus sp. MK568070 Cultured in Oil Refinery Wastewater

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