Evaluation of Wound Healing and Antibacterial Properties of Methanolic Root Extract of Stephania Dinklagei (Engl.) Diels

Abstract

Background: Stephania dinklagei Diels (Engl.) is used in folkloric medicine in Southeastern Nigeria for treatment of wounds and some bacterial-associated infections. This study evaluated the wound healing and antibacterial potential of Stephania dinklagei to validate or invalidate its folkloric use.
 Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of methanolic extract of S. dinklagei root (MESDR) against Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella spp. was determined by macrobroth dilution. The extract at 20% and 10% concentrations were dosed orally to rats at 300mg/kg body weight (bw) in incision and dead space wound healing model to determine wound tensile strength and granulation tissue weight, respectively. Same extract concentrations were applied topically in excision wound model to determine the rate of wound contraction and epithelialization. Activities of superoxide dismutase (SOD), catalase (CAT), and the levels of total protein (TP), malondialdehyde (MAL), hydroxyproline (HYP) and hexosamine (HEX) in excision wound biopsies were determined at days 7 and 14 post wounding (pw). In the excision wound model, the extract concentrations were compared with gentamicin sulphate.
 Results: The MIC of S. dinklagei extract against P. aeruginosa, S. aureus, B. subtilis, E. coli and Klebsiella spp. were 8mg/ml, 3 mg/ml, 5mg/ml, 6mg/ml and 7mg/ml, respectively, while the corresponding MBC were 10 mg/ml, 5 mg/ml, 7mg/ml, 8mg/ml, and 9 mg/ml, respectively. The 20% extract concentration gave significantly (P0.05) at day 14 pw. HYP level of the extract-treated groups significantly (P

Pharmacological and histopathological assessment of <i>Phyllobotyron spathulatum</i> Müll.Arg.) in rats

“…Documented works abound on the use of plant-based products to cure various ailments (Edeoga et al, 2005;Rodriguez, 2021;Yang et al, 2021;Onoja et al, 2023). Carrageenan-induced rat paw oedema has been widely used for the discovery and evaluation of anti-inflammatory drugs (Mujumdar and Misar, 2004;Udegbunam et al, 2015;. The anti-inflammatory effects of the leaves of P. spathulatum showed a mean decrease in paw volume of the rats treated with different concentrations of the leaf extract of the plant as well as the aspirin-treated group when compared with the control group.…”