Evaluation of wound healing effect of alginate film containing Aloe vera gel and cross-linked with zinc chloride

Abstract

Purpose To develop a new wound dressing composed of alginate and Aloe vera gel and cross-linked with zinc ions. Methods The aloe-alginate film was characterized using scanning electron microscopy (SEM), swelling profile, mechanical properties, polysaccharide content and X-ray diffraction (XRD). Thirty Wistar rats were divided in two groups a) treated with aloe-alginate film and b) control (treated with sterile gauze). Wound contraction measurements and hystological analysis were performed on 7th, 14th and 21st days after wound surgery. Results The aloe-alginate film presented adequated mechanical resistance and malleability for application as wound dressing. There was no statistical difference in wound contraction between two groups. Histological assay demonstrated that aloe-alginate film presented anti-inflammatory activity, stimulated angiogenesis on proliferative phase and a more significant increased in collagen type I fibers and decreased type III fibers which promoted a mature scar formation when compared to control. Conclusions The aloe-alginate film showed adequate physicochemical characteristics for wound dressing applications. The in vivo assay demonstrated that aloe-alginate film enhanced the healing process of incisional skin wounds.

<p><strong>Efektivitas wound healing sheet ekstrak Aloe vera dan albumin telur ayam kampung (Gallus domesticus) terhadap penyembuhan luka insisi gingiva</strong></p><p><strong><em>Wound healing sheet with Aloe vera extract and free-range chicken egg albumin (Gallus domesticus) for healing of gingival incisions</em></strong></p>

“…Aloe vera yang dibuat dalam sediaan lembaran memiliki sifat fleksibel dan elastis sehingga membuat wound healing sheet mudah digunakan dan tidak menimbulkan rasa sakit ketika dipasang atau dilepas. 15 Tekstur sediaan lembaran antara pemberian ekstrak 50% dan 100% berbeda. Wound healing sheet ekstrak 50% memiliki tekstur yang lebih lembut sedangkan ekstrak 100% lebih kasar.…”

“…yang meneliti efek dari aloe-alginate film cross-linked with zinc chloride sebagai wound dressing bisa memodulasi fase inflamasi, meningkatkan angiogenesis pada fase proliferatif dan meningkatkan kolagen tipe I dan ini dipengaruhi oleh senyawa yang terkandung dalam Aloe vera. 15 Penelitian yang sama yang dilakukan oleh Paques dkk. menyimpulkan bahwa aloe-alginate film cross-linked with calcium memiliki efek yang lebih besar dalam modulasi fase inflamasi dan kolagenesis dibandingkan dengan alginat tanpa gel Aloe vera.…”

“…Teknik electrospinning ini menggunakan kekuatan elektrostatis untuk mengubah serat polimer ke skala nano atau mikro. 5,6,15 Salah satu kekurangan dari teknik electrospinning adalah peralatan mahal dan sulit ditemukan sehingga diperlukan alternatif yang lebih sederhana misalnya dengan pembuatan lem jaringan dalam bentuk hidrogel. 24 Pembuatan lem jaringan dalam bentuk hidrogel lebih mudah dibuat karena alat yang sederhana.…”

Marine Polysaccharides for Wound Dressings Application: An Overview

“…Due to its high biocompatibility and non-irritating properties, AV is often used in combination with MPs to develop wound dressings. Many studies have shown that MPs wound dressings incorporating AV enhance scar inhibition by promoting wound contraction and orderly deposition of collagen [58,275,342]. Other natural agents also have been shown to enhance collagen repair, reduce collagen deposition and accelerate healing by impeding the growth of gelatinous scar tissue, such as heparin, essential oils, silk etc.…”

Current Trends in Advanced Alginate-Based Wound Dressings for Chronic Wounds

“…Because of its hydrophilic nature, alginate is capable to take multiple forms [ 11 , 12 , 13 , 14 ] (beads, blends, dressings, electrospun scaffolds, flexible fibers, films, foams, gels, hydrogels, injections, microparticles, microspheres, nanoparticles, polyelectrolyte complex, powders, ropes, sheets, sponges) that could be applied on post-traumatic wounds or exuding wounds (ulcers) while decreasing contamination [ 15 , 16 , 17 , 18 ], either as a stand-alone biomaterial, or in various combinations.…”