Evaluation of α-Glucosidase Activity in Dog Semen and its Use in Fertility Diagnosis

Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot ( Armeniaca sibirica L. Lam.) pulp

“…The ␣-glucosidase inhibition activity was determined by ELISA reader in a 96-well plate using PNPG as substrate (Cremonesi, Torti, Pecile, Groppetti, & Biondi, 2003;Kima, Hyunb, & Kim, 2011). The assay mixture (240 L) contained 120 L of 0.5 mol/L phosphate buffer (pH 6.7), 50 L of substrate solution (0.9133 mg/mL PNPG in 0.5 mol/L phosphate buffer), 50 L of enzyme solution (25 mg/mL ␣-glucosidase in 0.5 mol/L phosphate buffer containing 0.2% BSA), and 20 L of the indicated concentration gradient of the samples.…”