Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot ( Armeniaca sibirica L. Lam.) pulp

Cui, Jie; Gu, Xin; Wang, Fengjun; Ouyang, Jie; Jian-zhong, Wang

doi:10.1016/j.carbpol.2014.12.065

Cited by 74 publications

(26 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The EPS molecular weight and its distribution were measured using GPC/MALL56. This process was performed on an infusion system (Waters 515 HPLC Unit Pump) fitted with one gel column (Shodex OHpak SB-806M HQ) at 35 °C as column temperature and the combined detectors (Wyatt-DAWN HELEOS® eighteen-angle laser light scattering and Wyatt-Optilab rex refractive index detector).…”

Section: Methodsmentioning

confidence: 99%

Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice

Zhang

Zhou

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

The fibroblast cell line of 3T3-L1 was used as a cell model for screening and evaluating the feasibility of probiotic components in improving animal lipid metabolisms. The extracts from 12 Lactobacillus strains caused significantly reduced triacylglycerol (TAG) accumulation but with severe inflammation induction in 3T3-L1 adipocytes. Interestingly, exopolysaccharides (EPS) from LGG (Lactobacillus rhamnosus GG) significantly decreased the TAG accumulation without any inflammation. The anti-obesity effect of EPS was confirmed in high-fat-diets feeding mice. Fat pads of mice injected with EPS (50 mg/kg) every two days for two weeks were significantly reduced with much smaller adipocytes, compared with the counterparts. The levels of TAG and cholesterol ester in liver, as well as serum TAG, were decreased in EPS injected mice. In addition, down-regulated inflammation was observed in adipose tissue and liver. Interestingly, the expression of TLR2 in adipose tissue and 3T3-L1 cells was significantly increased by EPS addition. Moreover, the reverse of TAG accumulation in TLR2 knockdown 3T3-L1 in the presence of EPS confirmed that the inhibition effect of EPS on adipogenesis was mediated by TLR2. EPS from LGG has the potential for therapeutic development to intervene lipid metabolic disorders in mammals.

show abstract

Section: Methodsmentioning

confidence: 99%

Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice

Zhang

Zhou

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…; Cui et al . ; Ramchandran and Shah ; Lata Ramchandran and Shah ). To further ensure the effects of probiotics in vivo , an appropriate and stable T2DM animal model were widely used, which was induced by a high‐fat diet and low‐dose injections of streptozotocin (STZ) (Mu et al .…”

Section: Introductionmentioning

confidence: 99%

Effects ofLactobacillus plantarumCCFM0236 on hyperglycaemia and insulin resistance in high-fat and streptozotocin-induced type 2 diabetic mice

Wang

Yin

et al. 2016

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…-Glucosidase is an important carbohydrate digestion enzyme that has been considered as a therapeutic approach to adjust postprandial hyperglycaemia [29]. Many studies have focused on seeking effective and safe -glucosidase inhibitors from natural products to develop functional foods to combat diabetes [32][33][34][35][36].…”

Section: -Glucosidase Inhibitory Activitymentioning

confidence: 99%

Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro

Jian

Qiao

Chen

et al. 2017

Journal of Chemistry

View full text Add to dashboard Cite

Miracle fruit (Synsepalum dulcificum) has been well known and studied for its unique taste-modifying ability. In this study, the monosaccharide composition, molecular weight (Mw), and in vitro bioactivities (antioxidant, α-glucosidase inhibition) of polysaccharides from the seeds (MFP-S) and leaves (MFP-L) of miracle fruit were investigated. The results showed that MFP-S was a homogeneous polysaccharide (Mw 2804 Da) with glucose. MFP-L displayed three fractions (92093, 1496, and 237 Da) consisting of rhamnose, arabinose, galactose, glucose, and xylose. Moreover, the antioxidant and α-glucosidase inhibition of MFP-L were significantly greater than those of MFP-S. The α-glucosidase inhibition of MFP-L was remarkably better than the positive control, acarbose (an antidiabetes drug). More specifically, the 50% inhibitory concentration (IC50) values of α-glucosidase activities for MFP-S, MFP-L, and acarbose were 33, 0.01, and 1 mg mL−1, separately. Therefore, MFP-L can be developed as a functional factor with both antioxidant and antidiabetes activities in food applications.

show abstract

Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot ( Armeniaca sibirica L. Lam.) pulp

Cited by 74 publications

References 28 publications

Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice

Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice

Effects ofLactobacillus plantarumCCFM0236 on hyperglycaemia and insulin resistance in high-fat and streptozotocin-induced type 2 diabetic mice

Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities In Vitro

Contact Info

Product

Resources

About