Evaluation on Anticancer Effect Against HL-60 Cells and Toxicity in vitro and in vivo of the Phenethyl Acetate Isolated from a Marine Bacterium Streptomyces griseus

Abstract

We previously identified Streptomyces griseus as an anti-cancer agent . In this study, we isolated compounds from S. griseus and evaluated their anticancer effect and toxicity in vitro and in vivo. Preparative centrifugal partition chromatography (CPC) was used to obtain three compounds, cyclo, cyclo( L -Phe-trans-4-hydroxy-L -Pro) and phenethyl acetate (PA). We chose PA, which had the highest anticancer activity, as a target compound for further experiments. PA induced the formation of apoptotic bodies, DNA fragmentation, DNA accumulation in G 0 /G 1 phase, and reactive oxygen species (ROS) formation. Furthermore, PA treatment increased Bax/Bcl-xL expression, activated caspase-3, and cleaved poly-ADP-ribose polymerase (PARP) in HL-60 cells. Simultaneous evaluation in vitro and in vivo, revealed that PA exhibited no toxicity in Vero cells and zebrafish embryos. We revealed, for the first time, that PA generates ROS, and that this ROS accumulation induced the Bcl signaling pathway.

Protective effect of gallic acid derivatives from the freshwater green alga Spirogyra sp. against ultraviolet B-induced apoptosis through reactive oxygen species clearance in human keratinocytes and zebrafish

“…Many studies have been conducted to find the bioactive extracts or active ingredients derived from natural sources, particularly having polyphenol structure including hydroxyl groups on aromatic rings, which can modulate the generation of UVB induced-ROS (Samarakoon et al 2013, Farooqi et al 2015, Lee et al 2015b, 2015c, 2015d, Alves et al 2016, Fernando et al 2017a, 2017b.…”

Diphlorethohydroxycarmalol (DPHC) Isolated from the Brown Alga Ishige okamurae Acts on Inflammatory Myopathy as an Inhibitory Agent of TNF-α

“…Marine organisms are rich sources of structurally diverse bioactive compounds with various biological activities. Especially, marine brown algae contain a variety of bioactive substances including phlorotannins, polysaccharide, and pigments and exhibit different biological activities [7,8] and potential health benefits such as antioxidant activity [9], anti-inflammatory activity [10], anti-cancer activity [11], antidiabetic activity [12], antihypertensive effects [13], and anti-obesity activity [14]. Especially, phlorotannins found only in brown algae, is a polyphenolic compound, the most important group of biological substances that determine the value of functional foods or pharmaceuticals [15].…”

Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo

“…The nuclear morphology of the cells was evaluated using the cell-permeable DNA dye, Hoechst 33342 (Lee et al 2015). Cells were treated with various concentrations of PFF-A (5.0, 20.8, and 41.5 μM) and 2 h later 15 mM of AAPH was added to the culture.…”

“…Cell viability was estimated using MTT assay according to the method described by Lee et al (2015). MTT stock have been proved were shown to be responsible for antioxidant, antimicrobial, anti-obesity, anti-elastase, antiallergy, anti-inflammatory, and anti-human immunodeficiency virus activities (Artan et al 2008, Le et al 2009, Eom et al 2012, Kang et al 2012b, Ko et al 2013, Kim 2014.…”