Evaluation on the antiviral activity of ribavirin against Micropterus salmoides rhabdovirus (MSRV) in vitro and in vivo

Yang, Fei; Song, Kaige; Zhang, Zhongyu; Cheng, Chen; Wang, Gao‐Xue; Yao, Jiayun; Ling, Fei

doi:10.1016/j.aquaculture.2021.736975

Cited by 39 publications

(26 citation statements)

References 31 publications

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“…Recently, Wang et al reported drug screening for anti-MSRV from 10 FDA-approved common antiviral drugs, namely, 1-adamantanamine, 1-adamantanamine, 1-adamantanamine, ribavirin, moroxydine hydrochloride, acyclovir, ganciclovir, vidarabine monohydrate, isoprinosine, and N 2,9-diacetylguanine. The results showed that ribavirin exhibited the best antiviral activity, with a 98.33% inhibition rate at 17.32 μM, and significantly increased cell viability [ 15 ]. Searching for drugs to treat emerging viral diseases among FDA-approved antiviral drugs is a quick and useful approach, because the scalable production, antiviral mechanism of action, and side-effects of these drugs are well studied.…”

Section: Discussionmentioning

confidence: 99%

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Synthesized Magnolol Derivatives Improve Anti-Micropterus salmoides Rhabdovirus (MSRV) Activity In Vivo

Jin

Yang

Zhang³

et al. 2022

Viruses

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Micropterus salmoides rhabdovirus (MSRV) is a primary viral pathogen in largemouth bass aquaculture, which leads to tremendous economic losses yearly. Currently, there are no approved drugs for the treatment and control of this virus. Our previous studies screened the herb Magnolia officinalis from many traditional Chinese medicines, and we isolated and identified magnolol as its main active compound against multiple rhabdoviruses, including MSRV. On the basis of the structure–activity relationship and pharmacophore model of magnolol, two new magnolol derivatives, namely, hydrogenated magnolol and 2,2′-dimethoxy-magnolol, were designed and synthesized. Their anti-MSRV activities were systematically investigated both in vitro and in vivo. By comparing the half-maximal inhibitory concentration (IC50), it was found that hydrogenated magnolol possessed a higher anti-MSRV activity than magnolol and 2,2′-dimethoxy-magnolol, with an IC50 of 13.37 μM. Furthermore, hydrogenated magnolol exhibited a protective effect on the grass carp ovary (GCO) cell line by reducing the cytopathic effect induced by MSRV. Further studies revealed that hydrogenated magnolol did not directly impact virions or interfere with MSRV adsorption. It worked within the 6–8 h of the phase of virus replication. In vivo treatment of MSRV infection with magnolol and hydrogenated magnolol showed that they significantly improved the survival rate by 44.6% and 62.7%, respectively, compared to MSRV-infected groups. The viral load measured by the expression of viral glycoprotein in the organs including the liver, spleen, and kidney also significantly decreased when fish were intraperitoneally injected at a dose of 20 mg/kg. Altogether, the structural optimization of magnolol via hydrogenation of the propylene groups increased its anti-MSRV activity both in vitro and in vivo. These results may provide a valuable reference for anti-MSRV drug discovery and development in aquaculture.

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Section: Discussionmentioning

confidence: 99%

“…The MSRV strain (MSRV-FJ985) was isolated, identified, and stored in our laboratory with GenBank access no. MT818233.1 [ 15 ]. The grass carp ovary (GCO) cells were kindly provided by the Zhejiang Institute of Freshwater Fisheries (Huzhou, China).…”

Section: Methodsmentioning

confidence: 99%

Synthesized Magnolol Derivatives Improve Anti-Micropterus salmoides Rhabdovirus (MSRV) Activity In Vivo

Jin

Yang

Zhang³

et al. 2022

Viruses

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“…Micropterus salmoides rhabdovirus (MSRV) (FJ985) was originally isolated from largemouth bass and propagated on grass carp ovary (GCO) cells in a 25°C incubator (Yang et al., 2021). The complete genome of MSRV was uploaded to the NCBI database (GenBank: MT818233.1).…”

Section: Methodsmentioning

confidence: 99%

“…The workflow of the experimental design is shown in Figure 1(c). According to previous studies, the method was described as follows: GCO cells with a density of 1 × 10 5 /wells were cultured in 12‐well cell culture plate and infection with MSRV (10 3 × TCID 50 /0.1 mL) for 2 h at 25°C (Yang et al., 2021). The supernatant containing the viral particles was aspirated and GCO cells were washed 3 times with phosphate‐buffered saline (PBS) (0.1 M).…”

Section: Methodsmentioning

confidence: 99%

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Quinoline, with the active site of 8‐hydroxyl, efficiently inhibits Micropterus salmoides rhabdovirus (MSRV) infection in vitro and in vivo

Yang

Zhang

et al. 2022

Journal of Fish Diseases

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Micropterus salmoides rhabdovirus (MSRV) is an significant pathogen that causes high mortality and related economic losses in bass aquaculture. There is no effective or approved therapy to date. In this study, we evaluated the anti-MSRV effects of 22 quinoline derivatives in grass carp ovary (GCO) cells. Among these compounds, 8-hydroxyquinoline exhibited valid inhibition in decreasing MSRV nucleoprotein gene expression levels of 99.3% with a half-maximal inhibitory concentrations (IC 50 ) value of 4.66 μM at 48 h. Moreover, 8-hydroxyquinoline significantly enhanced a protective effect in GCO cells by reducing the cytopathic effect (CPE). By comparing the anti-MSRV activity of 22 quinoline derivatives, we found that 8-hydroxyquinoline possessed the efficient active site of 8-hydroxyl and inhibited MSRV infection in vitro.For in vivo studies, 8-hydroxyquinoline via intraperitoneal injection exhibited an antiviral effect in MSRV-infected largemouth bass by substantially enhancing the survival rate by 15.0%. Importantly, the viral loads in the infected largemouth bass notably reduced in the spleen on the third days post-infection. Overall, 8-hydroxyquinoline was considered to be an efficient agent against MSRV in aquaculture.

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Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway

Wang,

Deng,

et al. 2023

Aquaculture and Fisheries

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Evaluation on the antiviral activity of ribavirin against Micropterus salmoides rhabdovirus (MSRV) in vitro and in vivo

Cited by 39 publications

References 31 publications

Synthesized Magnolol Derivatives Improve Anti-Micropterus salmoides Rhabdovirus (MSRV) Activity In Vivo

Synthesized Magnolol Derivatives Improve Anti-Micropterus salmoides Rhabdovirus (MSRV) Activity In Vivo

Quinoline, with the active site of 8‐hydroxyl, efficiently inhibits Micropterus salmoides rhabdovirus (MSRV) infection in vitro and in vivo

Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway

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