Evalution of Antioxidant Activity of Various Herbal Folk Medicines

Miser-Salihoglu, Ece; Akaydın, Galip; Caliskan-Can, Emel; Yardım-Akaydin, Sevgi

doi:10.4172/2155-9600.1000222

Cited by 17 publications

(10 citation statements)

References 69 publications

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“…The 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay was conducted according to the protocol described by Miser-Salihoglu et al (2013) with some modifications. ABTS radical cation (ABTS·) was produced via reacting ABTS stock solution (7 mM) and potassium persulfate (2.45 mM) for 24 h before the assay.…”

Section: Methodsmentioning

confidence: 99%

Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines

et al. 2017

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Streptomyces colonosanans MUSC 93JT, a novel strain isolated from mangrove forest soil located at Sarawak, Malaysia. The bacterium was noted to be Gram-positive and to form light yellow aerial and vivid yellow substrate mycelium on ISP 2 agar. The polyphasic approach was used to determine the taxonomy of strain MUSC 93JT and the strain showed a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. Phylogenetic and 16S rRNA gene sequence analysis indicated that closely related strains include Streptomyces malachitofuscus NBRC 13059T (99.2% sequence similarity), Streptomyces misionensis NBRC 13063T (99.1%), and Streptomyces phaeoluteichromatogenes NRRL 5799T (99.1%). The DNA–DNA relatedness values between MUSC 93JT and closely related type strains ranged from 14.4 ± 0.1 to 46.2 ± 0.4%. The comparison of BOX-PCR fingerprints indicated MUSC 93JT exhibits a unique DNA profile. The genome of MUSC 93JT consists of 7,015,076 bp. The DNA G + C content was determined to be 69.90 mol%. The extract of strain MUSC 93JT was demonstrated to exhibit potent antioxidant activity via ABTS, metal chelating, and SOD assays. This extract also exhibited anticancer activity against human colon cancer cell lines without significant cytotoxic effect against human normal colon cells. Furthermore, the chemical analysis of the extract further emphasizes the strain is producing chemo-preventive related metabolites. Based on this polyphasic study of MUSC 93JT, it is concluded that this strain represents a novel species, for which the name Streptomyces colonosanans sp. nov. is proposed. The type strain is MUSC 93JT (= DSM 102042T = MCCC 1K02298T).

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Section: Methodsmentioning

confidence: 99%

Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines

et al. 2017

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“…This showed that components which can be extracted from the leaves with polar solvents are more responsible for antioxidant activities. Phenols containing several hydroxyl groups would play an important role for antioxidant activity, since they transfer hydrogen easily to scavenge free radicals and convert themselves into stable phenoxide radicals (Miser-Salihoglu et al, 2013). Therefore, these data indicates that P. lanceolata plant can be used as a good source of antioxidative compounds that control the level of free radical formation in the body.…”

Section: Antioxidant Potential Determinationmentioning

confidence: 92%

“…Plantago lanceolata known by a common name ribwort plantain is one of the species of genus plantago. The Leaves of this plant have been used for traditional medicinal activities in various countries (Miser-Salihoglu et al, 2013;Taskova et al, 2002). In most part of the world including Ethiopia, the plant has not been cultivated on piece of land or large farms since it is considered as weed.…”

Section: Introductionmentioning

confidence: 99%

Studies on antioxidant and antibacterial activities of crude extracts of Plantago lanceolata leaves

Int¹

2019

Preprint

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In this study, three different antioxidant activity quantification methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), reducing power, FRAP and peroxide value determination including total phenolic, TP, total flavonoid, TF assays of crude leaf extracts of Plantago lanceolata were carried out. The extracts showed significant activities in all antioxidant assays in a concentration dependent manner. The crude extracts were found to possess higher ferric reducing activity ranging from 336.58±0.052 mgAAE/100g (90% methanol) to 172.94±0.032 mgAAE/100g (petroleum ether) and DPPH activity varied from 90.6% (90% methanol) to 36.04% (petroleum ether). Strong correlation of TPC with AEACDPPH and AEACFRAP (R2 = 0.9033 and R2 = 0.8538 respectively) implied that compounds in the extract were proficient to scavenge the DPPH free radical and reducing ferric ions into ferrous ions. Moreover, peroxide values of the extracts were obtained and varied from 19.8 meq/kg (90% methanol) to 379 meq/kg(petroleum ether) and 3.1meq/kg (90% methanol) to 119 meq/kg (petroleum ether) at 70 oC and room temperature respectively. Therefore, P. lanceolata leaf extracts as compared to the control showed effective activity in delaying oxidation of the oil. Antimicrobial capacity evaluation of crude extracts against different gram-positive and gram-negative organisms was also evaluated and a higher degree of antimicrobial activity was observed of crude extracts with MIC and MBC values in the range of 6.25 to 25% were also observed for the crude extracts.

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“…A method based on the description of Miser-Salihoglu E. et al was applied [26]. The examination was performed on a FLUOstar Optima BMG Labtech plate-reader with 96well microplates.…”

Section: Dpph Assaymentioning

confidence: 99%

Triterpenes and Phenolic Compounds From the Fungus <em>Fuscoporia torulosa</em>: Isolation, Structure Determination and Biological Activity

Béni¹,

Dékány²,

Sárközy³

et al. 2021

Preprint

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Investigation of the methanol extract of the poroid fungus Fuscoporia torulosa resulted in the isolation of a novel triterpene, fuscoporic acid (1) together with inoscavin A and its previously undescribed Z isomer (2 and 3), 3,4-dihydroxy-benzaldehide (4), osmundacetone (5), senexdiolic acid (6), natalic acid (7), and ergosta-7,22-diene-3-one (8). The structures of fungal compounds were determined on the basis of NMR and MS spectroscopic analysis, as well as molecular modelling studies. Compounds 1, 6-8 were examined for their antibacterial properties on resistant clinical isolates, and cytotoxic activity on human colon adenocarcinoma cell lines. Compound 8 was effective against Colo 205 (IC50 11.65±1.67 µM), Colo 320 (IC50 8.43±1.1 µM) and MRC-5 (IC50 7.92±1.42 µM) cell lines. Potentially synergistic relationship was investigated between 8 and doxorubicin, which revealed a synergism between the examined compounds with a combination index (CI) at the 50% growth inhibition dose (ED50) of 0.521±0.15. Several compounds (1, and 6-8) were tested for P‐glycoprotein modulatory effect in Colo 320 resistant cancer cells, but none of the compounds proved to be effective in this assay. Fungal metabolites 2-5 were evaluated for their antioxidant activity using the oxygen radical absorbance capacity (ORAC) and DPPH assays. Compounds 4 and 5 proved to possess considerable antioxidant effect with EC50 0.25±0.01 (DPPH) and 12.20±0.92 mmol TE/g (ORAC). The current article provides valuable information on both chemical and pharmacological profiles of Fuscoporia torulosa, paving the way for future studies with this species.

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Evalution of Antioxidant Activity of Various Herbal Folk Medicines

Cited by 17 publications

References 69 publications

Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines

Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines

Studies on antioxidant and antibacterial activities of crude extracts of Plantago lanceolata leaves

Triterpenes and Phenolic Compounds From the Fungus <em>Fuscoporia torulosa</em>: Isolation, Structure Determination and Biological Activity

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