Evalution of benomyl and carbendazim in the vivo aneuploidy/micronucleus assay in BDF1 mouse bone marrow

Sarrif, Awni M.; Bentley, Karin S.; Fu, Lei; Neil, Rita M.; Reynolds, Vincent L.; Stahl, Ralph G.

doi:10.1016/0027-5107(94)90018-3

Cited by 44 publications

(19 citation statements)

References 38 publications

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“…Carbendazim is a known aneugen, but it remains unclear whether it is a clastogen. 42,43 In this work in Figure 1, it is shown that carbendazim does not have clastogen properties when applied alone. Similar to our results, it was shown before by SCGE (single cell gel electrophoresis) assay that carbendazim applied individually does not inflict DNA breaks.…”

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confidence: 91%

Carbendazim combined with imazalil or cypermethrin potentiate DNA damage in hepatocytes of mice

et al. 2011

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Traces of pesticides imazalil, cypermethrin and carbendazim are detected in plants used for human consumption. To explore whether their application in oral combinations will induce DNA breaks in hepatocytes, a subchronic in vivo experiment was performed in Swiss mice. Doses of 10 mg kg(-1) of imazalil (im) and cypermethrin (cy), and 20 mg kg(-1) of carbendazim (car) and their combinations (im, 10 mg kg(-1) + cy, 10 mg kg(-1); im, 10 mg kg(-1) + car, 20 mg kg(-1); car, 20 mg kg(-1) + cy, 10 mg kg(-1)) were applied daily for 28 days. Afterward, DNA damage in hepatocytes was evaluated by comet assay. Individually, imazalil and cypermethrin damaged DNA at alkali-labile sites, while the tail moment (TM) of carbendazim alone was similar to control but with higher tail length. In combination with carbendazim clastogen, properties of imazalils and cypermethrins were potentiated compared to all other treatments and control. There were pronounced sex differences in pattern of fragmentation between treated groups. Higher long tail nuclei (LTN) in females indicate that certain cells in females were especially prone to total nucleus disintegration. Due to synergistic effects, low environmentally present concentrations of imazalil and cypermethrin in food, and especially their mixtures with carbendazim have genotoxic potential that could be particularly dangerous over prolonged exposure in mammalian organism.

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confidence: 91%

Carbendazim combined with imazalil or cypermethrin potentiate DNA damage in hepatocytes of mice

et al. 2011

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“…Development of significantly longer comet tails in birds of 1000 mg/kg group suggested a break of DNA strands. Benomyl and carbendazim have been reported by some workers to produce micronuclei in polychromic erythrocytes and normochromic erythrocytes (Sarrif et al 1994;Bianchi-Santamaria et al 1997). A benomyl-induced increase in micronuclei in polychromatic erythrocytes has been reported at 1000 mg/kg but not at 500 mg/kg (Seiler 1976).…”

Section: Discussionmentioning

confidence: 99%

“…Many reports have described benomyl and carbendazim to give negative results in bacterial reverse gene mutation assays and/or bacterial DNA damage/repair tests (McCarroll et al 2002). However, bone marrow micronucleus assays showed that benomyl and carbendazim resulted in significantly increased micronucleated polychromatic erythrocytes (Seiler 1976;Sarrif et al 1994). Apart from domestic and wild mammals, birds living in the same ecological system and searching feed in the fields treated with benomyl are also exposed to this compound.…”

Section: Benomyl Pathology Quails Testes Dna Damage Comet Assaymentioning

confidence: 99%

Pathological Effects of Benomyl in Male Japanese Quails (Coturnix japonica)

et al. 2008

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Khan M. Z., Sajjad-Ul-Hassan, F. Mahmood, Q. M. Khan, G. Muhammad , I. Javed: Pathological Effects of Benomyl in Male Japanese Quails (Coturnix japonica). Acta Vet. Brno 2008, 77: 209-216.The purpose of this experimental study was to observe the pathological and genotoxic effects of a systemic fungicide, Benomyl [methyl 1-(butyl carbamoyl)-2 benzimidazole carbamate] in male Japanese quails (Coturnix japonica). Benomyl (Benlate ® DoPont 50% w/w benomyl) at dose rates of 0, 100, 400 and 1000 mg/kg for 8 consecutive days was administered into crops of quails kept in different groups. A significant decrease (p < 0.05) in feed intake, body weight and relative weight of testes was observed in benomyl-administered quails in a dose related manner. The size of testes decreased in benomyl treated quails. Microscopically, seminiferous tubules of testes exhibited a decreased number of spermatocytes, necrotic spermatids and syncytial cell formation. The number of quails developing testicular alterations increased in a dose-related pattern. These alterations reversed following a withdrawal of benomyl with the exception of the 1000 mg benomyl group, where these persisted till the end of the experiment (56 th day). The mean tail length (µm) of the comets produced by leukocytes in single cell gel electrophoresis of 0, 100 and 400 mg dose level groups were 3.48, 3.24 and 3.29, respectively. The mean tail length of comets of the 1000 mg dose level group was 12.03 µm and significantly higher (p < 0.01) than in all other groups indicating DNA strand breaks at this dose level. These results suggested gonadotoxic effects of benomyl (Benlate ® ) and also a possible genotoxic effect at 1000 mg/kg level in Japanese quails.

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“…benomyl, and vincristine [40][41][42]. Thus, when studying aneugen effects in vivo and in vitro, one should take into account the mechanism of their action and plan the research strategy according to the proposed dose-effect model [36].…”

Section: Agents Inducing Aneuploidy In Cells Of Rodents and Humansmentioning

confidence: 99%

Interphase cytogenetics in estimation of genomic mutations in somatic cells

2005

Russ J Genet

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Evalution of benomyl and carbendazim in the vivo aneuploidy/micronucleus assay in BDF1 mouse bone marrow

Cited by 44 publications

References 38 publications

Carbendazim combined with imazalil or cypermethrin potentiate DNA damage in hepatocytes of mice

Carbendazim combined with imazalil or cypermethrin potentiate DNA damage in hepatocytes of mice

Pathological Effects of Benomyl in Male Japanese Quails (Coturnix japonica)

Interphase cytogenetics in estimation of genomic mutations in somatic cells

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