Evanescent field effect–based nanobiosensors for agro-environmental and food safety

Adányi, Nóra; Majer-Baranyi, Krisztina; Székacs, András

doi:10.1016/b978-0-12-804301-1.00011-4

Cited by 11 publications

(10 citation statements)

References 123 publications

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“…These CR values are in good agreement with the corresponding values reported for our ELISA system for ZON [ 29 ]; however, the detection sensitivity of the current OWLS immunosensor exceeds that of the ELISA by five orders of magnitude. Such outstanding improvement in the detection range of an OWLS immunosensor compared to the corresponding ELISA has been reported [ 59 , 78 ].…”

Section: Resultsmentioning

confidence: 97%

“…A magnetic nanotag-based immunoassay [ 55 ] and a multiplexed quantum dot immunochromatographic assay [ 56 ] allowed the parallel detection of ZON in the presence of other mycotoxins. Label-free biosensors on the basis of antibodies [ 57 , 58 , 59 , 60 ], aptamers [ 58 , 61 , 62 , 63 , 64 , 65 , 66 , 67 ], or molecularly imprinted polymers [ 68 , 69 , 70 , 71 ] as recognition elements have also been developed with various signal amplification and detection routes involved, and the range of sensoric detection techniques is expanding [ 72 ]. Thus, a surface plasmon resonance (SPR) biosensor has been developed for the simultaneous detection of four mycotoxins, with an LOD below 0.2 ng/mL for ZON [ 73 ], a gold nanoparticle-amplified imaging SPR (iSPR) biosensor allowed an LOD for ZON of 59.2 pg/mL in multiplex mycotoxin determination [ 74 ], a method of total internal reflection ellipsometry (TIRE) allowed detection of ZON at concentrations as low as 0.1 ng/mL [ 75 ], and electrochemical sensors resulted in LODs of 0.15–0.25 pg/mL [ 42 , 60 ].…”

Section: Introductionmentioning

confidence: 99%

“…Immunosensors based on the technique of optical waveguide lightmode spectroscopy (OWLS) have been applied with success to detect different molecules, and gained importance in environmental and food analysis [ 59 , 76 , 77 ]. In the current study, an OWLS immunosensor has been developed for the determination of ZON in maize samples.…”

Section: Introductionmentioning

confidence: 99%

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Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

Székács

Adányi

Szendrő

et al. 2021

Toxins

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Novel optical waveguide lightmode spectroscopy (OWLS)-based immunosensor formats were developed for label-free detection of Fusarium mycotoxin zearalenone (ZON). To achieve low limits of detection (LODs), both immobilised antibody-based (direct) and immobilised antigen-based (competitive) assay setups were applied. Immunoreagents were immobilised on epoxy-, amino-, and carboxyl-functionalised sensor surfaces, and by optimising the immobilisation methods, standard sigmoid curves were obtained in both sensor formats. An outstanding LOD of 0.002 pg/mL was obtained for ZON in the competitive immunosensor setup with a dynamic detection range between 0.01 and 1 pg/mL ZON concentrations, depending on the covalent immobilisation method applied. This corresponds to a five orders of magnitude improvement in detectability of ZON relative to the previously developed enzyme-linked immonosorbent assay (ELISA) method. The selectivity of the immunosensor for ZON was demonstrated with structural analogues (α-zearalenol, α-zearalanol, and β-zearalanol) and structurally unrelated mycotoxins. The method was found to be applicable in maize extract using acetonitrile as the organic solvent, upon a dilution rate of 1:10,000 in buffer. Thus, the OWLS immunosensor method developed appears to be suitable for the quantitative determination of ZON in aqueous medium. The new technique can widen the range of sensoric detection methods of ZON for surveys in food and environmental safety assessment.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

Székács

Adányi

Szendrő

et al. 2021

Toxins

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“…EW fluorescence biosensors rely on measurements in which the surface fluorophores are selectively excited. Such geometric confinement facilitates the preferential signal enhancement from the fluorophores on the surface while allowing the minimisation of undesirable background noise from the bulk sample [157]. The commercialisation of several waveguide-based sensors has spurred the development of a profuse variety of biosensors for several types of clinically relevant biomolecules [156].…”

Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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“…Label-free optical biosensor techniques based on evanescent field effects are of increasing interest for agro-environmental safety in monitoring the quality of food and animal feed [1]. Evanescent waves (or fields) created at interfaces between two transparent media having different refractive indices (RIs) in optical devices such as waveguides propagate along the interfaces with their intensity decaying rapidly away from the interfaces.…”

Section: Introductionmentioning

confidence: 99%

An Optical Planar Waveguide-Based Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

Nabok

Al-Jawdah

Gémes

et al. 2021

Toxins

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A planar waveguide (PW) immunosensor working as a polarisation interferometer was developed for the detection of mycotoxin zearalenone (ZON). The main element of the sensor is an optical waveguide consisting of a thin silicon nitride layer between two thicker silicon dioxide layers. A combination of a narrow waveguiding core made by photolithography with an advanced optical set-up providing a coupling of circular polarised light into the PW via its slanted edge allowed the realization of a novel sensing principle by detection of the phase shift between the p- and s-components of polarised light propagating through the PW. As the p-component is sensitive to refractive index changes at the waveguide interface, molecular events between the sensor surface and the contacting sample solution can be detected. To detect ZON concentrations in the sample solution, ZON-specific antibodies were immobilised on the waveguide via an electrostatically deposited polyelectrolyte layer, and protein A was adsorbed on it. Refractive index changes on the surface due to the binding of ZON molecules to the anchored antibodies were detected in a concentration-dependent manner up to 1000 ng/mL of ZON, allowing a limit of detection of 0.01 ng/mL. Structurally unrelated mycotoxins such as aflatoxin B1 or ochratoxin A did not exert observable cross-reactivity.

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Evanescent field effect–based nanobiosensors for agro-environmental and food safety

Cited by 11 publications

References 123 publications

Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

Recent Progress in Optical Sensors for Biomedical Diagnostics

An Optical Planar Waveguide-Based Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

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