Evidence-based severity assessment: Impact of repeated versus single open-field testing on welfare in C57BL/6J mice

Bodden, Carina; Siestrup, Sophie; Palme, Rupert; Kaiser, Sylvia; Sachser, Norbert; Richter, S. Helene

doi:10.1016/j.bbr.2017.08.029

Cited by 26 publications

(20 citation statements)

References 43 publications

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“…No loss of body weights was detected and, hence, we conclude that multiple habituation days to the least did not influence this general welfare parameter. Comparable to this result, Bodden et al investigated the impact of repeated versus single open-field testing on welfare in C57BL/6J mice and also found no body weight changes or other signs of compromised welfare [48]. The anxiety of an animal is usually connected to time spent in the center and time spent…”

Section: Plos Onementioning

confidence: 96%

Repeatability analysis improves the reliability of behavioral data

et al. 2020

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Section: Plos Onementioning

confidence: 96%

Repeatability analysis improves the reliability of behavioral data

et al. 2020

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“…To gain a picture as comprehensive as possible of the individuals' welfare, body weight development and several well-established welfare measures [e.g., see (27,28)] were systematically investigated. In particular, fecal corticosterone metabolites, anxiety-like and exploratory behavior, spatial learning abilities, nesting behavior as well as home cage behavior were studied.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Different Feeding Routines on Welfare in Laboratory Mice

et al. 2020

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The accepted norm in most laboratories around the globe is feeding laboratory mice an ad libitum diet, although several health impairments are well-established. In contrast, reducing the animals' body weight by feeding them less food once per day (referred to as 24 h schedule) has been shown to enhance life span and reduce disease susceptibility. Against this background, this study aimed at systematically investigating the effects of different feeding routines. Therefore, three feeding routines were compared to the standard ad libitum feeding and effects on body weight development and welfare were investigated in male C57BL/6J mice. In particular, a 24 h schedule group, an AUTO group, characterized by an automated supply of small pieces of food all over the day, and a 4 h removal group, characterized by daily removal of food for 4 h, were studied. While the removal of food for 4 h per day did not lead to a reduction of body weight, and hence is unlikely to prevent negative effects of overfeeding, both the 24 h schedule group and the AUTO group led to the aspired body weight reduction. In the AUTO group, however, higher levels of corticosterone metabolites and stereotypies were observed, implying a rather negative impact on welfare. By contrast, no distinct negative effects of a 24 h schedule were found. Studies like this underline the general need for evidence-based severity assessments of any procedure involving living animals.

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“…The results of this study suggest that long-term chronic administration of fipronil induces hyperactivity in animals under moderate stress conditions like in the open-field test (as compared to the MWM inducing high stress in animals due to adverse stimuli [42,43]). Compared to the control We averaged the mean speed displayed by the animals throughout the training phase and probe trial for each session of MWM testing in order to reveal evolution of mean speed throughout the testing session or between treatment groups ( Figure 7B).…”

Section: Fipronil Treatment Modifies General Activity In Micementioning

confidence: 75%

“…The results of this study suggest that long-term chronic administration of fipronil induces hyperactivity in animals under moderate stress conditions like in the open-field test (as compared to the MWM inducing high stress in animals due to adverse stimuli [42,43]). Compared to the control group, the fipronil-treated animals displayed significantly higher values for total distance travelled and relative time spent mobile in the OFT after 43 weeks of treatment.…”

Section: Fipronil Treatment Modifies General Activity In Micementioning

confidence: 75%

Long-Term Fipronil Treatment Induces Hyperactivity in Female Mice

Koslowski

Latapy

Auvray

et al. 2020

IJERPH

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Fipronil is an insecticide widely used for veterinary and agricultural purposes. While its insecticidal properties mostly rely on its high affinity antagonistic activity on insect γ aminobutyric acid (GABA) receptors, fipronil and its main metabolite fipronil sulfone nevertheless display non-negligible affinity for mammalian GABA A receptor. As several environmental toxicants have been shown to raise the risk of developing various neurodegenerative disorders, the aim of this study was to evaluate whether long-term low dose administration of fipronil could lead to cognitive deficiencies. Our results indicate that long-term fipronil treatment leads to behavioral perturbations in mice, indicating an accumulative effect of sustained exposure to low dose of fipronil. Although no memory impairment was observed during the course of our study, we noticed a significant hyperlocomotion behavior after 43 weeks of weekly fipronil administration, which is consistent with its direct effect on the GABAergic system.

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Evidence-based severity assessment: Impact of repeated versus single open-field testing on welfare in C57BL/6J mice

Cited by 26 publications

References 43 publications

Repeatability analysis improves the reliability of behavioral data

Repeatability analysis improves the reliability of behavioral data

The Effects of Different Feeding Routines on Welfare in Laboratory Mice

Long-Term Fipronil Treatment Induces Hyperactivity in Female Mice

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