Evidence for active acetylcholine metabolism in human amniotic epithelial cells: applicable to intracerebral allografting for neurologic disease

Sakuragawa, Norio; Misawa, Hidemi; Ohsugi, Kojune; Kakishita, Kouji; Ishii, Takashi; Thangavel, Ramasamy; Tohyama, Jun; Elwan, Mohamed A.; Yokoyama, Yasushi; Okuda, Osamu; Arai, Hajime; Ogino, Ikuko; Satô, Kiyoshi

doi:10.1016/s0304-3940(97)00570-3

Cited by 91 publications

(60 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The localization of ChAT IR in the submucosal and myenteric plexi is consistent with other reports that employed immunocytochemistry on whole mounts (Furness et al 1984(Furness et al ,1985Schemann et al 1993Schemann et al ,1995Mann et al 1995), including studies that successfully used 1.B3.9B3 (Schemann et al 1993(Schemann et al ,1995. In addition, ChAT IR and activity in endothelia of various tissues have been previously described (Parnavelas et al 1985;Gonzalez and Santos-Benito 1987;Milner et al 1989), and there are reports of ChAT in epithelial cells (Grando et al 1993;Sakuragawa et al 1997) and lymphocytes (Rinner and Schauenstein 1993).…”

Section: Discussionsupporting

confidence: 88%

“…In this context, it is interesting to note that acetylcholinesterase activity is present in some epithelia, including large bowel (Sine et al 1991), epidermis (Grando et al 1993), and amniotic epithelial cells (Sakuragawa et al 1997), and that ChAT is also capable of hydrolyzing acetylcholine (Molenaar 1990). Skin and amniotic epithelial cells have been shown both to contain ChAT and to metabolize acetylcholine (Grando et al 1993;Sakuragawa et al 1997). However, the literature does not provide an explanation for the observed pattern of stronger staining in the jejunal villi and weaker or negative staining in the jejunal crypts, ileum, and colon.…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Choline Acetyltransferase (ChAT) Immunoreactivity in Paraffin Sections of Normal and Diseased Intestines

Ratcliffe

Desa

Dixon

et al. 1998

J Histochem Cytochem.

View full text Add to dashboard Cite

SUMMARYThere is increasing interest in localizing nerves in the intestine, especially specific populations of nerves. At present, the usual histochemical marker for cholinergic nerves in tissue sections is acetylcholinesterase activity. However, such techniques are applicable only to frozen sections and have uncertain specificity. Choline acetyltransferase (ChAT) is also present in cholinergic nerves, and we therefore aimed to establish a paraffin section immunocytochemical technique using an anti-ChAT antibody. Monoclonal anti-choline acetyltransferase (1.B3.9B3) and a biotin-streptavidin detection system were used to study the distribution of ChAT immunoreactivity (ChAT IR) in paraffin-embedded normal and diseased gastrointestinal tracts from both rats and humans. Optimal staining was seen after 6-24 hr of fixation in neutral buffered formalin and overnight incubation in 1 g/ml of 1.B3.9B3, with a similar distribution to that seen in frozen sections. In the rat diaphragm (used as a positive control), axons and motor endplates were ChAT IR. Proportions of ganglion cells and nerve fibers in the intramural plexi of both human and rat gastrointestinal tracts were also ChAT IR, as well as extrinsic nerve bundles in aganglionic segments of Hirschsprung's disease. Mucosal cholinergic nerves, however, were not visualized. In addition, non-neuronal cells such as endothelium, epithelium, and inflammatory cells were ChAT IR. We were able to localize ChAT to nerves in formalin-fixed, paraffin-embedded sections. The presence of ChAT IR in non-neuronal cells indicates that this method should be used in conjunction with other antibodies. Nevertheless, it proves to be a useful technique for studying cholinergic neuronal distinction in normal tissues and pathological disorders.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Figurementioning

confidence: 99%

Choline Acetyltransferase (ChAT) Immunoreactivity in Paraffin Sections of Normal and Diseased Intestines

Ratcliffe

Desa

Dixon

et al. 1998

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…Some authors have related HAEC neurotrophic function (i.e. acetylcholine and catecholamine synthesis and release) to their ability to sustain the early phases of neuroepithelium formation and neural development of the embryo [17]. In addition these cells can survive after implantation into a rat model of Parkinson's disease [18] or after spinal cord injury [19] and differentiate into neuron-like cells after transplantation [9].…”

Section: Discussionmentioning

confidence: 99%

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

et al. 2006

View full text Add to dashboard Cite

“…The observed therapeutic effects are probably mediated by secretion of diffusible factors, including neurotransmitters (Elwan & Sakuragawa 1997;Sakuragawa et al 1997Sakuragawa et al , 2001) and neurotrophic and growth factors (Koizumi et al 2000;Uchida et al 2000).…”

Section: Brainmentioning

confidence: 99%

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Abdulrazzak

Moschidou

Jones

et al. 2010

J. R. Soc. Interface.

130

118

View full text Add to dashboard Cite

Foetal stem cells (FSCs) can be isolated during gestation from many different tissues such as blood, liver and bone marrow as well as from a variety of extraembryonic tissues such as amniotic fluid and placenta. Strong evidence suggests that these cells differ on many biological aspects such as growth kinetics, morphology, immunophenotype, differentiation potential and engraftment capacity in vivo. Despite these differences, FSCs appear to be more primitive and have greater multi-potentiality than their adult counterparts. For example, foetal blood haemopoietic stem cells proliferate more rapidly than those found in cord blood or adult bone marrow. These features have led to FSCs being investigated for pre-and post-natal cell therapy and regenerative medicine applications. The cells have been used in pre-clinical studies to treat a wide range of diseases such as skeletal dysplasia, diaphragmatic hernia and respiratory failure, white matter damage, renal pathologies as well as cancers. Their intermediate state between adult and embryonic stem cells also makes them an ideal candidate for reprogramming to the pluripotent status.

show abstract

Evidence for active acetylcholine metabolism in human amniotic epithelial cells: applicable to intracerebral allografting for neurologic disease

Cited by 91 publications

References 14 publications

Choline Acetyltransferase (ChAT) Immunoreactivity in Paraffin Sections of Normal and Diseased Intestines

Choline Acetyltransferase (ChAT) Immunoreactivity in Paraffin Sections of Normal and Diseased Intestines

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Contact Info

Product

Resources

About