Evidence for aluminum-binding erythropoietin by size-exclusion chromatography coupled to electrothermal absorption atomic spectrometry

Atomic spectrometry update. Clinical and biological materials, foods and beverages

“…Unfortunately, many EPO formulations are contaminated with Al. Viega et al 104 coupled SEC with ETAAS to study the binding of Al to protein species in EPO formulations. With optimised conditions to separate EPO from serum albumin (also present as a major protein species), the authors quantitatively determined Al in 1 mL aliquots of the column eluate and established that Al was only associated with the fractions corresponding to the EPO peak.…”

Binding study of lysozyme with Al(III) using chemiluminescence analysis

“…Significant attention has been paid to the establishment of an accurate, sensitive and effective method for investigating the binding behavior of protein with Al(III). Different methods have been reported for studying the interaction of protein with Al(III), for example, glutamate dehydrogenase with Al(III) using electrochemistry and circular dichroism , erythropoietin with Al(III) using size‐exclusion chromatography‐absorption atomic spectrometry and trypsin with Al(III) using Raman and infrared spectroscopy , showing that the binding of Al(III) to the active sites of protein induced the changes in α helices, β sheets and random coils. To date, studying the binding behavior of lysozyme with Al(III) using flow injection–chemiluminescence (FI–CL) analysis has not been reported.…”

Atomic spectrometry update. Clinical and biological materials, foods and beverages

“…An alternative sample introduction system, a dual drop-ondemand aerosol generator, and a calibration strategy based on the dosing frequency were applied to the determination of Cs, Li, Mo, Sb and Sr in urine in the concentration range of 8-122 mg L À1 42 Unfortunately, many EPO formulations are contaminated with Al. Viega et al104 coupled SEC with ETAAS to study the binding of Al to protein species in EPO formulations. With optimised conditions to separate EPO from serum albumin (also present as a major protein species), the authors quantitatively determined Al in 1 mL aliquots of the column eluate and established that Al was only associated with the fractions corresponding to the EPO peak.…”