Evidence for apical Na+/H+ exchanger in bovine main pancreatic duct

Marteau, C.; Silviani, V.; Ducroc, Robert; Crotté, C.; Gérolami, A

doi:10.1007/bf02063234

Cited by 22 publications

(12 citation statements)

References 19 publications

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“…Because the pancreatic duct secretes HCO 3 -and Na + , NHEs are not expected to be present in the LM. However, using perfused ducts, we and others have reported NHE activity in the LM of pancreatic ducts of different species (4,5). The proteins mediating this activity, their regulation, and their physiological significance are unknown.…”

Section: Introductionmentioning

confidence: 85%

Na+-dependent transporters mediate HCO3– salvage across the luminal membrane of the main pancreatic duct

et al. 2000

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Section: Introductionmentioning

confidence: 85%

Na+-dependent transporters mediate HCO3– salvage across the luminal membrane of the main pancreatic duct

et al. 2000

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“…Pancreatic juice at resting or low flow rates is acidic and contains high level of CO 2 , indicating that an active H + secreting mechanism is functional at this state (118, 263). NHE2 and NHE3 are localized at the luminal membrane of the large-sized mouse pancreatic and salivary glands ducts (33, 234, 250, 264, 326). The role of NHE2 is not clear since deletion of NHE2 in mice had no obvious disease phenotype or altered pH homeostasis and HCO 3 − metabolism in several organs expressing NHE2 (384), including the pancreatic (234) and salivary glands ducts (98, 250).…”

Section: Ductal Fluid and Hco3− Secretionmentioning

confidence: 99%

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Lee

Ohana

Park

et al. 2012

Physiological Reviews

349

455

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Fluid and HCO3−secretion is a vital function of all epithelia and is required for the survival of the tissue. Aberrant fluid and HCO3−secretion is associated with many epithelial diseases, such as cystic fibrosis, pancreatitis, Sjögren's syndrome, and other epithelial inflammatory and autoimmune diseases. Significant progress has been made over the last 20 years in our understanding of epithelial fluid and HCO3−secretion, in particular by secretory glands. Fluid and HCO3−secretion by secretory glands is a two-step process. Acinar cells secrete isotonic fluid in which the major salt is NaCl. Subsequently, the duct modifies the volume and electrolyte composition of the fluid to absorb the Cl−and secrete HCO3−. The relative volume secreted by acinar and duct cells and modification of electrolyte composition of the secreted fluids varies among secretory glands to meet their physiological functions. In the pancreas, acinar cells secrete a small amount of NaCl-rich fluid, while the duct absorbs the Cl−and secretes HCO3−and the bulk of the fluid in the pancreatic juice. Fluid secretion appears to be driven by active HCO3−secretion. In the salivary glands, acinar cells secrete the bulk of the fluid in the saliva that is driven by active Cl−secretion and contains high concentrations of Na+and Cl−. The salivary glands duct absorbs both the Na+and Cl−and secretes K+and HCO3−. In this review, we focus on the molecular mechanism of fluid and HCO3−secretion by the pancreas and salivary glands, to highlight the similarities of the fundamental mechanisms of acinar and duct cell functions, and to point out the differences to meet gland-specific secretions.

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“…Apical Na + /H + exchange has been implicated in this phenomenon in a murine (341) and bovine pancreas (188), and initially attributed to the apically expressed NHE2 and NHE3 (169). However, the luminal Na + -dependent H + efflux in Nhe2 −/− ducts is normal, suggesting NHE2 plays no role in HCO 3 − salvage in the ducts.…”

Section: Na+/h+ Exchange In the Exocrine And Endocrine Pancreasmentioning

confidence: 99%

SLC9 Gene Family: Function, Expression, and Regulation

Kiela

2018

Comprehensive Physiology

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The Slc9 family of Na /H exchangers (NHEs) plays a critical role in electroneutral exchange of Na and H in the mammalian intestine as well as other absorptive and secretory epithelia of digestive organs. These transport proteins contribute to the transepithelial Na and water absorption, intracellular pH and cellular volume regulation as well as the electrolyte, acid-base, and fluid volume homeostasis at the systemic level. They also influence the function of other membrane transport mechanisms, affect cellular proliferation and apoptosis as well as cell migration, adherence to the extracellular matrix, and tissue repair. Additionally, they modulate the extracellular milieu to facilitate other nutrient absorption and to regulate the intestinal microbial microenvironment. Na /H exchange is inhibited in selected gastrointestinal diseases, either by intrinsic factors (e.g., bile acids, inflammatory mediators) or infectious agents and associated bacterial toxins. Disrupted NHE activity may contribute not only to local and systemic electrolyte imbalance but also to the disease severity via multiple mechanisms. In this review, we describe the cation proton antiporter superfamily of Na /H exchangers with a particular emphasis on the eight SLC9A isoforms found in the digestive tract, followed by a more integrative description in their roles in each of the digestive organs. We discuss regulatory mechanisms that determine the function of Na /H exchangers as pertinent to the digestive tract, their regulation in pathological states of the digestive organs, and reciprocally, the contribution of dysregulated Na /H exchange to the disease pathogenesis and progression. © 2018 American Physiological Society. Compr Physiol 8:555-583, 2018.

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Evidence for apical Na+/H+ exchanger in bovine main pancreatic duct

Cited by 22 publications

References 19 publications

Na+-dependent transporters mediate HCO3– salvage across the luminal membrane of the main pancreatic duct

Na+-dependent transporters mediate HCO3– salvage across the luminal membrane of the main pancreatic duct

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

SLC9 Gene Family: Function, Expression, and Regulation

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Evidence for apical Na+/H+ exchanger in bovine main pancreatic duct

Cited by 22 publications

References 19 publications

Na+-dependent transporters mediate HCO3– salvage across the luminal membrane of the main pancreatic duct

Na+-dependent transporters mediate HCO3– salvage across the luminal membrane of the main pancreatic duct

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO3−Secretion

SLC9 Gene Family: Function, Expression, and Regulation

Contact Info

Product

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Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion