EVIDENCE FOR EXTENDED MAINTENANCE OF THE CORPUS LUTEUM BY UTERINE INFUSION OF a RECOMBINANT TROPHOBLAST Α-Interferon (TROPHOBLASTIN) IN SHEEP

Martal, J.; Degryse, Eric; Charpigny, Gilles; Assal, Nasser-Eddine; Reinaud, Pierrette; Charlier, Madia; Gaye, Pierre; Lecocq, Jean‐Pierre

doi:10.1677/joe.0.127r005

Cited by 63 publications

(26 citation statements)

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“…Twice-daily infusions or injections of IFNT (Vallet et al 1988) or recombinant ovine (ro) IFNT (Martal et al 1990) into the uterus of sheep for 2-8 days extends luteal life span to 22-27 days post oestrus. Similarly, uterine vein infusion of roIFNT extends luteal life span in ewes (Bott et al 2010).…”

Section: N Forde and P Lonergan F36mentioning

confidence: 99%

Interferon-tau and fertility in ruminants

Forde¹,

Lonergan²

2017

Reproduction

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Establishment of pregnancy in domestic ruminants includes pregnancy recognition signalling by the conceptus, implantation and placentation. Despite the high fertilisation success rate in ruminants, a significant amount of embryo loss occurs, primarily during early gestation. Interferon-tau (IFNT), a type I interferon that is exclusively secreted by the cells of the trophectoderm of the ruminant conceptus, has been recognised as the primary agent for maternal recognition of pregnancy in ruminants. It produces its antiluteolytic effect on the corpus luteum by inhibiting the expression of oxytocin receptors in the uterine epithelial cells, which prevents pulsatile, luteolytic secretion of prostaglandin F2α by the uterine endometrium. While the importance of IFNT in maternal recognition of pregnancy and prevention of luteolysis in ruminants is unequivocal, important questions, for example, relating to the threshold level of IFNT required for pregnancy maintenance, remain unanswered. This paper reviews data linking IFNT with measures of fertility in ruminants. Reproduction (2017) 154 F33-F43

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Section: N Forde and P Lonergan F36mentioning

confidence: 99%

Interferon-tau and fertility in ruminants

Forde¹,

Lonergan²

2017

Reproduction

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“…Glandular epithelial cells and fibroblast cells prepared from each cow were treated for 30 min, 2 h, or 24 h with 100 ng/ml or 1 g/ml of ovine recombinant IFNT (64). Control cells were left untreated and washed with cold PBS, recovered by scrapping, and then centrifuged at 4°C for 2 min at 10,000 rpm to eliminate supernatant.…”

Section: Primary Cultures Of Bovine Endometrial Cells and Ifnt Treatmentmentioning

confidence: 99%

Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation

Mansouri-Attia

Aubert

Reinaud

et al. 2009

Physiological Genomics

153

148

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At implantation the endometrium undergoes modifications necessary for its physical interactions with the trophoblast as well as the development of the conceptus. We aim to identify endometrial factors and pathways essential for a successful implantation in the caruncular (C) and the intercaruncular (IC) areas in cattle. Using a 13,257-element bovine oligonucleotide array, we established expression profiles at day 20 of the estrous cycle or pregnancy (implantation), revealing 446 and 1,295 differentially expressed genes (DEG) in C and IC areas, respectively (false discovery rate ϭ 0.08). The impact of the conceptus was higher on the immune response function in C but more prominent on the regulation of metabolism function in IC. The C vs. IC direct comparison revealed 1,177 and 453 DEG in cyclic and pregnant animals respectively (false discovery rate ϭ 0.05), with a major impact of the conceptus on metabolism and cell adhesion. We selected 15 genes including C11ORF34, CXCL12, CXCR4, PLAC8, SCARA5, and NPY and confirmed their differential expression by quantitative RT-PCR. The cellular localization was analyzed by in situ hybridization and, upon pregnancy, showed gene-specific patterns of cell distribution, including a high level of expression in the luminal epithelium for C11ORF34 and MX1. Using primary cultures of bovine endometrial cells, we identified PTN, PLAC8, and CXCL12 as interferon-(IFNT) target genes and MSX1 and CXCR7 as IFNT-regulated genes, whereas C11ORF34 was not an IFNT-regulated gene. Our transcriptomic data provide novel molecular insights accounting for the biological functions related to the C or IC endometrial areas and may contribute to the identification of potential biomarkers for normal and perturbed early pregnancy.transcriptome; interferon-tau; pregnancy; cattle IN MAMMALS, the establishment and maintenance of pregnancy require a subtle and tightly regulated communication between the conceptus (embryo and embryonic annexes) and the maternal environment (85). The success of implantation relies on several essential steps including the adjustment of the uterine environment to support the development of the conceptus and the profound remodeling of the endometrium structure necessary for the apposition, adhesion, and invasion phases (36). In contrast to human and rodents, the invasion of the maternal tissue by the fetal tissue is very limited in ruminants (71) and leads to a synepitheliochorial placentation (86). Since the trophoblast appears to be intrinsically invasive in mammals (11), apposition, adhesion, and invasion processes are thought to be controlled by the endometrium (83). In mammalian species presenting an invasive implantation, decidua restrains the invasion of the embryo in a spatiotemporal manner (20). The expression and the regulation of some factors involved in the apposition, adhesion, and invasion aspects of implantation have been reported in ruminants (62, 79), but, overall, the comparative cascade of molecular mechanisms remains largely unknown.The sequence of events occ...

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“…IFN-~, previously named trophoblastin or trophoblast protein type 1 is a new class of type I IFN that has been described in sheep, goats and cows [8,9]. In these species, IFN-~is physiologically secreted by trophoblast as an antiluteolytic protein responsible for maternal recognition of pregnancy [10]. IFN-~exhibits antiviral activity, is weakly cytotoxic and has immunosuppressor effects towards human CD4-positive lymphocytes [11].…”

Section: Induction Of Type I Ifn Gene Expressionmentioning

confidence: 99%

Type I interferons: expression and signalization

Doly

Civas

Navarro

et al. 1998

CMLS, Cell. Mol. Life Sci.

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Type I interferon (IFN-A and IFN-B) genes encode a large family of multifunctional secreted proteins involved in antiviral defence, cell growth regulation and immune activation. These cytokines, as a consequence of their biological activities, have been established as effective therapeutic molecules for malignant and viral diseases. Virus infection is the main inducer leading to transient expression of type I IFN (A and B) and the antiviral response appears to proceed through a two-step pathway requiring, first, induction of type I IFN gene expression and, second, transcriptional activation by the synthesized IFN proteins, binding to their specific cell surface receptors, of a large number of genes. The proteins they encode are responsible, in part, for the pleiotropic multiple biological activities of the IFN. In this two-step pathway, the virus-induced IFN genes and the IFN-stimulated gene (ISG) expression seem to share common factors. Even if IFN-A genes are structurally related and very often coordinately induced in virus-infected cells, differences in the expression of the individual IFN-A messenger RNAs of the multigenic IFN-A gene family are observed in human as well as in murine cells, reflecting, in a particular cell type, the transcriptional activity of the corresponding promoter regions. Important studies on interferon regulatory factors and ISG factors have been made in the last decade. However, some factors involved in IFN-A gene regulation remain to be identified. Our goal has been to review the factors involved in the control of the type I IFN gene expression to understand the mechanisms of induction and repression of their transcription and to explain the properties of these cytokines through their signal transduction pathway.

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EVIDENCE FOR EXTENDED MAINTENANCE OF THE CORPUS LUTEUM BY UTERINE INFUSION OF a RECOMBINANT TROPHOBLAST Α-Interferon (TROPHOBLASTIN) IN SHEEP

Cited by 63 publications

References 0 publications

Interferon-tau and fertility in ruminants

Interferon-tau and fertility in ruminants

Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation

Type I interferons: expression and signalization

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