Evidence for membrane cholesterol as the common binding site for cereolysin, streptolysin O and saponin

Shany, Shraga; Bernheimer, Alan W.; Grushoff, Phyllis; Kim, Kwang-Shin

doi:10.1007/bf01686643

Cited by 102 publications

(44 citation statements)

References 23 publications

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“…O n e possible explanation for the observed cooperative phenomenon is that SBA disturbs the enterocyte apical membrane by binding to reactive sites a n d that this in turn increases the membrane sensitivity to soyasaponins. Such a mechanism is represented in Figure 5, where we have assumed a crenator effect (12) o f soyasaponins o n the membrane bilayera n effect similar t o that shown previously for saponins in general o n cell populations (9,11,13,14). Glycinin leakage into the epithelium would occur a t the points of saponin attack o n the membrane.…”

Section: Treatment Of Jejunal Samples With Soy Fractionsmentioning

confidence: 86%

Interactions of Soybean Lectin, Soyasaponins, and Glycinin with Rabbit Jejunal Mucosa In Vitro

Alvarez

Torres-Pinedo

1982

Pediatr Res

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SummaryMucosal samples from rabbit jejunum were incubated (30 min, 25°C) with ('251)glycinin in the presence of buffer, soybean lectin (50 pg/ml) soyasaponins (1 mglml), or both lectin and saponins.The mucosal uptake of (1251)glycinin was negligible with buffer, and increased progressively with additions of soybean lectin (P < 0.05), soyasaponins (P < 0.005), and both (P < 0.0001). The stimulation of uptake by lectin and saponins together was greater than the sum of their individual effects (P < 0.0005). The effect of soybean lectin on glycinin uptake was concentration dependent, reaching a maximum at approximately 50 pg/ml for the stimulation of uptake in the presence of saponins, and was inhibited by DGalNAc. Although the mechanisms involved in mucosal uptake of glycinin cannot be described from these data, we have assumed the presence of two independent pathways for lectin-stimulated and saponin-induced uptakes. In addition, we have proposed that soybean lectin, by binding to terminal galactoside sites at the enterocyte apical membrane, enhances a crenator effect of saponins that leads to increasing leakage of glycinin into the cell.

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Section: Treatment Of Jejunal Samples With Soy Fractionsmentioning

confidence: 86%

Interactions of Soybean Lectin, Soyasaponins, and Glycinin with Rabbit Jejunal Mucosa In Vitro

Alvarez

Torres-Pinedo

1982

Pediatr Res

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“…The decrease in the viability and inhibition of proliferation induced by LTS-4 was reversible in HSF cells across a broad spectrum of concentrations, but only at concentrations below 10 µg/ml in HTB140 cells. This effect may be due to the characteristic ability of saponins to bind with sterols, specifically with the membrane cholesterol [15,16], and the resulting permeabilization of the cell membrane. Galeotti et al [17] showed that the cholesterol content in hepatoma cell microsomal and plasma membranes is much higher than in normal cells.…”

Section: Discussionmentioning

confidence: 99%

The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

Galanty¹,

Michalik²,

Sędek³

et al. 2008

Cellular and Molecular Biology Letters

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Abstract:We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.

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“…Since cholesterol is an erythrocyte membrane constituent, it is assumed that hemolysis is inThe identity ot the H m values of tigogenin maltoside in dimethyl sulfoxide and isopropyl alcohol ( duced by the formation of an insoluble complex between the saponin (or sapogenin) and this constituent (30). The finding that cholesteryl maltoside is exactly as active as smilagenyl and tigogenyl maltosides is of special relevance to this theory.…”

Section: Values Of the Synthesized Glycoeides And The Aglycones And Cmentioning

confidence: 90%

Hemolytic Properties of Synthetic Glycosides

Segal

Shud

Milo-Goldzweig

1978

Journal of Pharmaceutical Sciences

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Evidence for membrane cholesterol as the common binding site for cereolysin, streptolysin O and saponin

Cited by 102 publications

References 23 publications

Interactions of Soybean Lectin, Soyasaponins, and Glycinin with Rabbit Jejunal Mucosa In Vitro

Interactions of Soybean Lectin, Soyasaponins, and Glycinin with Rabbit Jejunal Mucosa In Vitro

The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

Hemolytic Properties of Synthetic Glycosides

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