The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

Galanty, Agnieszka; Michalik, Marta; Sędek, Łukasz; Podolak, Irma

doi:10.2478/s11658-008-0013-x

Cited by 21 publications

(14 citation statements)

References 17 publications

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“…Figure demonstrates the composite trajectories of HSFs migrating on the glass surface noncovered (control) and covered with the various polyelectrolytes. The trajectories are shown as circular diagrams with the initial point of each trajectory placed at the origin of the plot as previously described . The average motility parameters calculated from the cell tracks are presented in Table .…”

Section: Resultsmentioning

confidence: 99%

“…The trajectories are shown as circular diagrams with the initial point of each trajectory placed at the origin of the plot as previously described. 22,32 The average motility parameters calculated from the cell tracks are presented in Table III. The final cell displacement shows the migration efficiency of cells into a wound area.…”

Section: Antibacterial Activities Of the Polyelectrolytes In Solutionmentioning

confidence: 99%

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Synthesis of strong polycations with improved biological properties

Wytrwał-Sarna

Koczurkiewicz

Wójcik

et al. 2013

J Biomedical Materials Res

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Poly(allylamine hydrochloride) (PAH) has found many applications both in biotechnology and biomedical fields. However, its high toxicity toward various mammalian cells significantly limits its effective usage. This study focuses on improving the biological properties of PAH by its modification to strong polyelectrolytes. The strong polycations were prepared by the direct quaternization of PAH amino groups or by the attachment of glycidyltrimethylammonium chloride to these groups. The biological properties, such as cytotoxicity toward human skin fibroblasts (HSFs), proliferation and migration of the cells on a polymeric surface, and antibacterial activities against two pathogenic bacteria, Staphylococcus aureus and Escherichia coli, were determined. All the modified polyelectrolytes are considerably less toxic to HSFs as compared to PAH. Moreover, the directly quaternized polycations are stronger biocides against S. aureus than the parent polymer. Contrary to PAH, thin films of the modified polyelectrolytes improve or do not affect HSFs proliferation and can stimulate cell migration into the wound, as was demonstrated using an in vitro model. The relationship between the structure of the modified polymers (amount and localization of the quaternary ammonium groups) and the biological activity is discussed. Due to the improved biological properties, the obtained polycations may be potentially useful for a variety of biotechnological and biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Antibacterial Activities Of the Polyelectrolytes In Solutionmentioning

confidence: 99%

Synthesis of strong polycations with improved biological properties

Wytrwał-Sarna

Koczurkiewicz

Wójcik

et al. 2013

J Biomedical Materials Res

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“…The trajectories of individual cells were evaluated from the changes in cell centroid location, as described previously [ 18 ]. For each cell, the following variables were determined [ 19 ]: (i) average speed of cell movement, i.e. the total length of cell trajectory/time of recording; (ii) the total length of cell displacement (μm), i.e.…”

Section: Methodsmentioning

confidence: 99%

Proton beam irradiation inhibits the migration of melanoma cells

et al. 2017

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PurposeIn recent years experimental data have indicated that low-energy proton beam radiation might induce a difference in cellular migration in comparison to photons. We therefore set out to compare the effect of proton beam irradiation and X-rays on the survival and long-term migratory properties of two cell lines: uveal melanoma Mel270 and skin melanoma BLM.Materials and methodsCells treated with either proton beam or X-rays were analyzed for their survival using clonogenic assay and MTT test. Long-term migratory properties were assessed with time-lapse monitoring of individual cell movements, wound test and transpore migration, while the expression of the related proteins was measured with western blot.ResultsExposure to proton beam and X-rays led to similar survival but the quality of the cell colonies was markedly different. More paraclones with a low proliferative activity and fewer highly-proliferative holoclones were found after proton beam irradiation in comparison to X-rays. At 20 or 40 days post-irradiation, migratory capacity was decreased more by proton beam than by X-rays. The beta-1-integrin level was decreased in Mel270 cells after both types of radiation, while vimentin, a marker of EMT, was increased in BLM cells only.ConclusionsWe conclude that proton beam irradiation induced long-term inhibition of cellular motility, as well as changes in the level of beta-1 integrin and vimentin. If confirmed, the change in the quality, but not in the number of colonies after proton beam irradiation might favor tumor growth inhibition after fractionated proton therapy.

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“…In cancer therapy, they were applied in combination with immunotoxins to increase the selectivity of the immunotoxin against cancer cells and to inure normal cells to its cytotoxic effect (Weng et al, 2012). On the other hand, direct pro-apoptotic, antiangiogenic, cytostatic and anti-invasive activity of saponosides has been demonstrated (Galanty et al, 2008;Kim et al, 2012;Podolak et al, 2013). They can enhance the effect of commonly used chemotherapeutic agents, suggesting possibilites for lowering their therapeutic doses and reducing the possible side-effects of chemotherapy The observations of therapeutic efficacy of the combinations of some natural products from Traditional Chinese Medicine with standard chemotherapeutic agents (Efferth et al, 2002;Hofman et al, 2012) warrant intensive research on the anti-cancer activity of saponosides and on the mechanisms of their interference with chemotherapeutics.…”

Section: Introductionmentioning

confidence: 99%

Lclet 4 enhances pro-apoptotic and anti-invasive effects of mitoxantrone on human prostate cancer cells - in vitro study.

Koczurkiewicz

Podolak²,

Wójcik³

et al. 2013

Acta Biochim Pol

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Triterpene saponosides are widely distributed plant secondary metabolites characterized by relatively low systemic cytotoxicity and a range of biological activities. These include anti-inflammatory, antimicrobial, vasoprotective and antitumor properties. In particular, the ability of saponins to enhance the cytotoxicity of chemotherapeutic drugs opened perspectives for their application in combined cancer chemotherapy. Here, we used human prostate cancer DU-145 cells as an in vitro model to elucidate the synergy of the interactions between biological activities of an oleanane type 13β,28-epoxy triterpene saponoside (Lclet 4) and mitoxantrone, which is a cytostatic drug commonly used in prostate cancer therapy. No cytotoxic or pro-apoptotic effect of Lclet 4 and mitoxantrone administered at the concentrations between 0.05 and 0.1 µg/ml could be seen. In contrast, cocktails of these agents exerted synergistic pro-apoptotic effects, accompanied by the activation of the caspase 3/7 system. This effect was paralleled by attenuating effects of Lclet 4/mitoxantrone cocktails on the invasive potential, metalloproteinase expression and motility of DU-145 cells. Multifaceted and additive effects of Lclet 4 and mitoxantrone on basic cellular traits crucial for prostate cancer progression indicate that the combined application of both agents at systemically neutral concentrations may provide the basis for new promising strategies of prostate cancer chemotherapy.

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The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

Cited by 21 publications

References 17 publications

Synthesis of strong polycations with improved biological properties

Synthesis of strong polycations with improved biological properties

Proton beam irradiation inhibits the migration of melanoma cells

Lclet 4 enhances pro-apoptotic and anti-invasive effects of mitoxantrone on human prostate cancer cells - in vitro study.

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