Evidence for phosphonate usage in the coral holobiont

Thomas, Simon; Burdett, Heidi L.; Temperton, Ben; Wick, Ryan R.; Snelling, D; McGrath, John W.; Quinn, John P.; Munn, Colin B.; Gilbert, Jack A.

doi:10.1038/ismej.2009.129

Cited by 31 publications

(25 citation statements)

References 11 publications

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“…have the ability to grow on Phns (Dyhrman et al, 2007). Moreover, the widespread distribution of the phnA gene, which encodes a phosphonoacetate hydrolase, underscores the potential importance of phosphonates in marine ecosystems, including corals (Gilbert et al, 2009;Martinez et al, 2009;Thomas et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Alternative pathways for phosphonate metabolism in thermophilic cyanobacteria from microbial mats

et al. 2010

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Synechococcus sp. represents an ecologically diverse group of cyanobacteria found in numerous environments, including hot-spring microbial mats, where they are spatially distributed along thermal, light and oxygen gradients. These thermophiles engage in photosynthesis and aerobic respiration during the day, but switch to fermentative metabolism and nitrogen fixation at night. The genome of Synechococcus OS-B 0 , isolated from Octopus Spring (Yellowstone National Park) contains a phn gene cluster encoding a phosphonate (Phn) transporter and a C-P lyase. A closely related isolate, Synechococcus OS-A, lacks this cluster, but contains genes encoding putative phosphonatases (Phnases) that appear to be active only in the presence of the Phn substrate. Both isolates grow well on several different Phns as a sole phosphorus (P) source. Interestingly, Synechococcus OS-B 0 can use the organic carbon backbones of Phns for heterotrophic growth in the dark, whereas in the light this strain releases organic carbon from Phn as ethane or methane (depending on the specific Phn available); Synechococcus OS-A has neither of these capabilities. These differences in metabolic strategies for assimilating the P and C of Phn by two closely related Synechococcus spp. are suggestive of niche-specific constraints in the evolution of nutrient assimilation pathways and syntrophic relationships among the microbial populations of the hotspring mats. Thus, it is critical to evaluate levels of various P sources, including Phn, in thermally active habitats and the potential importance of these compounds in the biogeochemical cycling of P and C (some Phn compounds also contain N) in diverse terrestrial environments.

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Section: Discussionmentioning

confidence: 99%

Alternative pathways for phosphonate metabolism in thermophilic cyanobacteria from microbial mats

et al. 2010

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“…They aroused extensive attention because of their potential in eliminating marine oil pollution, especially in polycyclic aromatic hydrocarbons (PAHs) degradation [3], [4], [7], [12]. Recently, Thalassospira were found to closely related with the phosphorus cycling in marine environments, especially in the oligotrophic open ocean environments [13], [14]. Additionally, some strains of this genus can produce thalassospiramides [15], [16], beta-galactosidase [17] and biosurfactants [18].…”

Section: Introductionmentioning

confidence: 99%

Multilocus Sequence Analysis for Assessment of Phylogenetic Diversity and Biogeography in Thalassospira Bacteria from Diverse Marine Environments

et al. 2014

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Thalassospira bacteria are widespread and have been isolated from various marine environments. Less is known about their genetic diversity and biogeography, as well as their role in marine environments, many of them cannot be discriminated merely using the 16S rRNA gene. To address these issues, in this report, the phylogenetic analysis of 58 strains from seawater and deep sea sediments were carried out using the multilocus sequence analysis (MLSA) based on acsA, aroE, gyrB, mutL, rpoD and trpB genes, and the DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) based on genome sequences. The MLSA analysis demonstrated that the 58 strains were clearly separated into 15 lineages, corresponding to seven validly described species and eight potential novel species. The DDH and ANI values further confirmed the validity of the MLSA analysis and eight potential novel species. The MLSA interspecies gap of the genus Thalassospira was determined to be 96.16–97.12% sequence identity on the basis of the combined analyses of the DDH and MLSA, while the ANIm interspecies gap was 95.76–97.20% based on the in silico DDH analysis. Meanwhile, phylogenetic analyses showed that the Thalassospira bacteria exhibited distribution pattern to a certain degree according to geographic regions. Moreover, they clustered together according to the habitats depth. For short, the phylogenetic analyses and biogeography of the Thalassospira bacteria were systematically investigated for the first time. These results will be helpful to explore further their ecological role and adaptive evolution in marine environments.

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“…(Panas, et al, 2006), Penicillium spp. (Forlani, et al, 2006), marine bacteria, and coral holobionts (Thomas, et al, 2010), establishing the presence of PhnA-mediated PnA hydrolysis in the microbial metabolome.…”

Section: Introductionmentioning

confidence: 99%

Structural and Mechanistic Insights into C-P Bond Hydrolysis by Phosphonoacetate Hydrolase

Agarwal

Borisova

Metcalf

et al. 2011

Chemistry & Biology

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SUMMARY Bacteria have evolved pathways to metabolize phosphonates as a nutrient source for phosphorus. In Sinorhizobium meliloti 1021, 2-aminoethylphosphonate is catabolized to phosphonoacetate, which is converted to acetate and inorganic phosphate by phosphonoacetate hydrolase (PhnA). Here we present detailed biochemical and structural characterization of PhnA that provides insights into the mechanism of C-P bond cleavage. The 1.35 Å resolution crystal structure reveals a catalytic core similar to those of alkaline phosphatases and nucleotide pyrophosphatases, but with notable differences such as a longer metal-metal distance. Detailed structure-guided analysis of active site residues and four additional co-crystal structures with phosphonoacetate substrate, acetate, phosphonoformate inhibitor, and a covalently-bound transition state mimic, provide insight into active site features that may facilitate cleavage of the C-P bond. These studies expand upon the array of reactions that can be catalyzed by enzymes of the alkaline phosphatase superfamily.

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Evidence for phosphonate usage in the coral holobiont

Cited by 31 publications

References 11 publications

Alternative pathways for phosphonate metabolism in thermophilic cyanobacteria from microbial mats

Alternative pathways for phosphonate metabolism in thermophilic cyanobacteria from microbial mats

Multilocus Sequence Analysis for Assessment of Phylogenetic Diversity and Biogeography in Thalassospira Bacteria from Diverse Marine Environments

Structural and Mechanistic Insights into C-P Bond Hydrolysis by Phosphonoacetate Hydrolase

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