Evidence for the Activation of Myeloperoxidase by f-Meth-Leu-Phe Prior to Its Release from Neutrophil Granulocytes

Zipfel, Matthias; Carmine, T.C.; Gerber, Claudia E.; Niethammer, D.; Bruchelt, Gernot

doi:10.1006/bbrc.1997.6257

Cited by 22 publications

(13 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, a loss of intracellular MPO characterized peripheral blood monocytes during this time. According to previous studies demonstrating that phagocyte activation leads to MPO exocytosis (19,20), we can assume that all these features likely reflect a shedding process and release reaction due to a massive cellular activation after blood contact with artificial surfaces of the extracorporeal circulation apparatus. Quantitative immune enzymatic assays of soluble forms of the above-mentioned molecules will confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 81%

Monitoring of monocyte functional state after extracorporeal circulation: A flow cytometry study

Sbrana

Parri

Filippis

et al. 2004

Cytometry Part B Clinical

View full text Add to dashboard Cite

Results: Cardiac surgery with CPB induced down-modulation of surface molecules expression on peripheral monocytes, especially at 24 h after CPB, for CD18, CD11a, and CD11b (P < 0.003) and for the CD15 adhesive cluster (P ‫؍‬ 0.0028) and HLA-DR (P < 0.001). At 4 h after CPB, downregulation was observed for CD14 (P ‫؍‬ 0.004), CD45 (P ‫؍‬ 0.014), and CD15 (P ‫؍‬ 0.0056). A loss of MPO was detected in venous peripheral (at 24 h after CPB, P ‫؍‬ 0.01) or coronary (at reperfusion, P < 0.02) blood. The CD15 cluster complex exhibited a down-modulation in coronary blood (at reperfusion, P ‫؍‬ 0.0003). Spontaneous intracellular production of IL-1␤, IL-6, and IL-8 decreased at 24 h after CPB (P < 0.05).Conclusions: The down-modulation of integrins and adhesive receptor expression and the loss of MPO suggest a strong activation and shedding reaction of circulating monocyte after CPB, further exacerbated by contact with coronary ischemic vessels. The changes of differentiation antigens may reflect the appearance of a partially immature population immediately after CPB. The reduced proinflammatory cytokine production, observed at 24 h after CPB, suggests a functional polarization of circulating monocytes.

show abstract

Section: Discussionmentioning

confidence: 81%

Monitoring of monocyte functional state after extracorporeal circulation: A flow cytometry study

Sbrana

Parri

Filippis

et al. 2004

Cytometry Part B Clinical

View full text Add to dashboard Cite

show abstract

“…As demonstrated by in test tube results, innate peroxidases, such as MPO in neutrophils and EPO in eosinophils, play a pivotal role in oxidative degradation of CNTs. Under natural inflammatory response, neutrophils are attracted and activated by chemoattractants at the inflammatory sites, and MPO is released thus being able to act both intra-and extra-cellularly [57]. H 2 O 2 , which originated from NADPH oxidase, forms inside the phagosome, activates MPO, and leads to the production of reactive enzymatic intermediates and oxidants ( e.g .…”

Section: In Vitro Degradation Of Cnts In Cellsmentioning

confidence: 99%

Peroxidase-mediated biodegradation of carbon nanotubes in vitro and in vivo

Kotchey

Zhao

Kagan

et al. 2013

Advanced Drug Delivery Reviews

162

147

View full text Add to dashboard Cite

As a result of their unique electronic, optical, and mechanical properties, carbon nanotubes (CNTs) have been implemented in therapeutic and imaging applications. In an idealized situation, CNTs would be disposed of after they transport their theranostic payloads. Biodegradation represents an attractive pathway for the eliminating of CNT carriers post-delivery and may be integral in catalyzing the release of the cargo from the delivery vehicle. Accordingly, recent research efforts have focused on peroxidase-driven biodegradation of CNTs. In this review, we not only summarize recent efforts to biodegrade CNTs in the test tube, in vitro, and in vivo, but also attempt to explore the fundamental parameters underlying degradation. Encouraged by the in vivo results obtained to date, we envision a future, where carbon-based nano-containers, which are specifically designed to target organs/cells, deliver their cargo, and biodegrade via peroxidase-driven mechanism, will represent an attractive therapeutic delivery option in nanomedicine.

show abstract

“…It has been shown that the MPXI is raised in patients with unstable angina and acute myocardial infarction,15following treatment with granulocyte colony stimulating factor (G-CSF),16 and in lymphoma transplant patients who develop bacteraemia 17. In addition, the MPXI has been used to study in vitro neutrophil activation18 and to detect the presence of myeloperoxidase deficiency 19…”

mentioning

confidence: 99%

Automated quantitation of circulating neutrophil and eosinophil activation in asthmatic patients

Leckie

2000

Thorax

View full text Add to dashboard Cite

Background-Asthma has been associated with eosinophil activation, measured in serum, sputum, bronchoalveolar lavage (BAL) fluid, and urine. A whole blood automated method was developed to assess eosinophil and neutrophil activity in terms of peroxidase content and cell morphology using the Bayer haematology analyser. The method was applied to an in vitro stimulation model when fMLP was added to whole blood and the samples were then analysed for changes in granularity and shape. In addition, cells stimulated with interleukin (IL)-8 were examined by electron microscopy. Methods-A cross sectional analysis was performed on venous blood from nonatopic, non-asthmatic normal subjects (n = 37), mild (n = 46) and symptomatic (n = 22) asthmatic patients on inhaled 2 agonist only, and more severe asthmatic patients (n = 17) on inhaled and oral corticosteroid therapy. Samples were analysed by the haematology analyser and peroxidase leucograms gated using the WinMDI software program. Results-There were significant diVerences in the amount of light scatter by the neutrophil populations in the symptomatic (p = 0.007) and severe asthmatic (p = 0.0001) groups compared with the control group. However, abnormalities in eosinophil populations were not observed. In vitro activation of whole blood with fMLP caused similar changes in neutrophil light scatter, suggesting that neutrophil activation is present in peripheral blood of symptomatic asthmatic patients. IL-8 caused a change in shape of the neutrophils seen using transmission electron microscopy. Conclusions-Evidence of neutrophil activation can be seen in whole blood from patients with asthma using a novel automated method. This may potentially be applied to other inflammatory diseases. (Thorax 2000;55:471-477)

show abstract

Evidence for the Activation of Myeloperoxidase by f-Meth-Leu-Phe Prior to Its Release from Neutrophil Granulocytes

Cited by 22 publications

References 12 publications

Monitoring of monocyte functional state after extracorporeal circulation: A flow cytometry study

Monitoring of monocyte functional state after extracorporeal circulation: A flow cytometry study

Peroxidase-mediated biodegradation of carbon nanotubes in vitro and in vivo

Automated quantitation of circulating neutrophil and eosinophil activation in asthmatic patients

Contact Info

Product

Resources

About