Evidence for the existence of CD34<sup>+</sup> angiogenic stem cells in human first‐trimester decidua and their therapeutic for ischaemic heart disease

Bai, Long; Sun, Lu; Chen, Wei; Liu, Kaiyu; Zhang, Chunfeng; Wang, Fei; Zhang, Gui-Huan; Huang, Ye; Li, Jing-Xuan; Gao, Ying; Sun, Xin; Liu, Wei; Du, Guoqing; Li, Ren‐Ke; Huang, Ming Jun; Tian, Hao

doi:10.1111/jcmm.15800

Cited by 11 publications

(10 citation statements)

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“…The incidence rate of heart failure after MI was more than 50% in 5 years. Previous studies have shown that BMSCs ( 17 ), embryonic stem cells ( 18 ), iPSCs ( 19 ), CD34 + angiogenic stem cells ( 10 ) and other MSCs promote blood flow reperfusion of infarcted myocardial tissue, induce angiogenesis in infarcted myocardium, and significantly improve cardiac function. Although MSC transplantation is considered as a promising therapeutic strategy for cardiac remodeling in ischemic heart disease, the effect is not satisfactory in clinical translational research.…”

Section: Discussionmentioning

confidence: 99%

“…After being separated by centrifugation (1,000 rpm/5 min), cells were resuspended in 4 ml DMEM/F12 medium containing 10% FBS, and cultured in an incubator of 37°C, 5% CO2. On the second day, DMSCs were observed in the culture bottle ( 10 ). Similarly, BMSCs were also obtained from female patients (20–30 years old) who needed median thoracotomy in cardiac surgery, stored into a bottle pre-filled with heparin later, quickly transferred to the laboratory for treatment.…”

Section: Methodsmentioning

confidence: 99%

“…BMSCs, the mononuclear cells layer, were obtained and washed twice. DMSCs and BMSCs were seeded in DMEM/F12 (HyClone) supplemented with 10% FBS (ScienCell) and cultured at 37°C in an atmosphere containing 5% CO 2 ( 10 ). Third-generation DMSCs/BMSCs were used for the following experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The infarct size of MI rats was measured as is previously reported ( 10 ). The ratio of the scar length and the circumference were measured by Masson's trichrome staining.…”

Section: Methodsmentioning

confidence: 99%

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Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism

Chen

Bai

et al. 2022

Front. Cardiovasc. Med.

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BackgroundCompared with bone marrow mesenchymal stem cells (BMSCs), decidual mesenchymal stem cells (DMSCs) are easy to obtain and exhibit excellent angiogenic effects, but their role in cell transplantation after myocardial infarction (MI) remains unclear.MethodsBMSCs and DMSCs were harvested from healthy donors. The effects of both cell types on angiogenesis were observed in vitro. Metabonomics analysis was performed to compare different metabolites and screen critical metabolic pathways. A murine model of acute myocardial infarction (AMI) was established, which was randomized into five groups (control, BMSC, DMSC, DMSC + ODCshRNA and BMSC + ODC consisting of 50 animals, equally divided into each group). The therapeutic effect of DMSCs on MI in rats was assessed based on neovascularization and cardiac remodeling.ResultsDMSCs exhibited a better angiogenic effect on human umbilical vein endothelial cells (HUVECs) than BMSCs in vitro. In addition, ornithine metabolism, which is associated with vascularization, was significantly increased in DMSCs. The transplantation of DMSCs in the rat MI model significantly enhanced angiogenesis of the infarct border area and improved cardiac remodeling and dysfunction postinfarction compared with BMSCs. Furthermore, inhibition of ornithine metabolism by silencing ornithine decarboxylase (ODC) in DMSCs partly abolished the benefits of DMSC transplantation.ConclusionCompared with BMSCs, DMSCs exhibited better efficacy in improving revascularization and heart remodeling post-MI via the activation of ODC-associated ornithine metabolism.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The infarct size of MI rats was measured as is previously reported ( 10 ). The ratio of the scar length and the circumference were measured by Masson's trichrome staining.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism

Chen

Bai

et al. 2022

Front. Cardiovasc. Med.

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“…They secrete soluble factors with paracrine effects. The therapeutic potential of hPMSCs has been showed in tissue damage repair after ischemic diseases including stroke, and myocardial and cerebral infarctions (7)(8)(9)(10)(11). These studies suggest that hPMSCs-based therapies might be developed for future clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Exosomal circ-RBM23 sponging miR-139-5p to promote liver regeneration through RRM2/AKT/mTOR pathway

Guo

et al. 2021

Preprint

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BackgroundExosomes are small nano-size membrane vesicles and are involved in intercellular interaction. Here, we examined if exosomes obtained from human placental stem cells promote liver regeneration after partial hepatectomy. MethodsExosomes generated from primary human placental stem cells were isolated and characterized. Cell co-culture model was used to clarify whether exosomes can induce hepatocytes proliferation in vitro . Partial hepatectomy mouse model was used to evaluate whether exosomes can promote hepatocytes proliferation in vivo . ResultsIt is found that human placental-derived stem cells exosomes (hPDSCs-exo) can induce hepatocyte proliferation in vitro and in vivo . Mechanistically, exosomal circ-RBM23 served as a ceRNA for miR-139-5p, regulated RRM2 and accelerated proliferation through AKT/mTOR pathways. Ablation of exosomal circ-RBM23 suppressed the proliferative effect of exosomes. ConclusionsThe hPMSCs exosomal circ-RBM23 stimulated cell proliferation and liver regeneration after 70% partial hepatectomy by regulated RRM2. Our findings highlight a potential novel therapeutic avenue for liver regeneration after hepatectomy.

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A Novel Conductive Polypyrrole‐Chitosan Hydrogel Containing Human Endometrial Mesenchymal Stem Cell‐Derived Exosomes Facilitated Sustained Release for Cardiac Repair

Yan,

Wang,

Wang

et al. 2024

Adv Healthcare Materials

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Myocardial infarction (MI) results in cardiomyocyte necrosis and conductive system damage, leading to sudden cardiac death and heart failure. Studies have shown that conductive biomaterials can restore cardiac conduction, but cannot facilitate tissue regeneration. This study aims to add regenerative capabilities to the conductive biomaterial by incorporating human endometrial mesenchymal stem cell (hEMSC)‐derived exosomes (hEMSC‐Exo) into poly‐pyrrole‐chitosan (PPY‐CHI), to yield an injectable hydrogel that could effectively treat MI. In vitro, PPY‐CHI/hEMSC‐Exo, compared to untreated controls, PPY‐CHI, or hEMSC‐Exo alone, alleviates H2O2‐induced apoptosis and promotes tubule formation, while in vivo, PPY‐CHI/hEMSC‐Exo improves post‐MI cardiac functioning, along with counteracting against ventricular remodeling and fibrosis. All these activities are facilitated via increased EGF/PI3K/AKT signaling. Furthermore, the conductive properties of PPY‐CHI/hEMSC‐Exo are able to re‐synchronize cardiac electrical transmission to alleviate arrythmia. Overall, PPY‐CHI/hEMSC‐Exo synergistically combines the cardiac regenerative capabilities of hEMSC‐Exo with the conductive properties of PPY‐CHI to improve cardiac functioning, via promoting angiogenesis and inhibiting apoptosis, as well as re‐synchronizing electrical conduction, to ultimately enable more effective MI treatment. Therefore, incorporating exosomes into a conductive hydrogel provides dual benefits in terms of maintaining conductivity, along with facilitating long‐term exosome release and sustained application of their beneficial effects.This article is protected by copyright. All rights reserved

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Evidence for the existence of CD34⁺ angiogenic stem cells in human first‐trimester decidua and their therapeutic for ischaemic heart disease

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 11 publications

References 35 publications

Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism

Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism

Exosomal circ-RBM23 sponging miR-139-5p to promote liver regeneration through RRM2/AKT/mTOR pathway

A Novel Conductive Polypyrrole‐Chitosan Hydrogel Containing Human Endometrial Mesenchymal Stem Cell‐Derived Exosomes Facilitated Sustained Release for Cardiac Repair

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Evidence for the existence of CD34+ angiogenic stem cells in human first‐trimester decidua and their therapeutic for ischaemic heart disease

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 11 publications

References 35 publications

Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism

Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism

Exosomal circ-RBM23 sponging miR-139-5p to promote liver regeneration through RRM2/AKT/mTOR pathway

A Novel Conductive Polypyrrole‐Chitosan Hydrogel Containing Human Endometrial Mesenchymal Stem Cell‐Derived Exosomes Facilitated Sustained Release for Cardiac Repair

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Evidence for the existence of CD34⁺ angiogenic stem cells in human first‐trimester decidua and their therapeutic for ischaemic heart disease