Evidence for the expression of estrogen receptors in osteogenic cells isolated from hen medullary bone

Hiyama, Shinji; Sugiyama, Toshie; Kusuhara, Seiji; Uchida, Takashi

doi:10.1016/j.acthis.2008.06.003

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…Using in situ hybridization, Imamura et al [8] demonstrated that osteoblasts express ER-α mRNA but not ER-β mRNA. Furthermore, a previous study we performed indicated that osteogenic cells derived from medullary bone express ER-α mRNA, but not ER-β mRNA, during bone formation [6]. These results suggest that estrogen, acting through ER-α but not ER-β, might influence medullary bone formation by osteogenic cells.…”

Section: Discussionmentioning

confidence: 66%

“…ERs have been found in osteoblasts, bone-lining cells, and bone marrow cells in these experimental animal models [15,16]. As found in a previous study, ER-α mRNA, but not ER-β mRNA, is expressed at constant levels throughout the differentiation of osteogenic cells isolated from medullary bone [6]. Although these experiments were performed during the bone formation period, expression of ER-α and/or ER-β during bone resorption has not been assessed.…”

Section: Introductionmentioning

confidence: 71%

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Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica)

et al. 2012

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The temporal expression of estrogen receptor (ER)-α and ER-β mRNA was examined in male Japanese quails. Femurs of quails receiving 17β-estradiol underwent RTPCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ER-α was already observed on day 0 and increased slightly during bone formation whereas ER-β was hardly detected throughout this process. After osteoclasts appeared on the medullary bone surface, this type of bone disappeared from the bone marrow cavity (days 7~15). ER-α expression simultaneously decreased slightly and ER-β levels remained very low. These results suggest that estrogen activity mediated by ER-α not only affects medullary bone formation but also bone resorption.

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 71%

Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica)

et al. 2012

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“…The high concentration of estrogen in combination with testosterone changes the function of osteoblasts to produce the medullary bone that provides a labile source of calcium for eggshell formation [7]. In this respect, it has been demonstrated that osteogenic cells on the surface of medullary bone express estrogen alpha receptors [8–10].…”

Section: Introductionmentioning

confidence: 99%

Integrative analysis of transcriptomic data related to the liver of laying hens: from physiological basics to newly identified functions

et al. 2019

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BackgroundAt sexual maturity, the liver of laying hens undergoes many metabolic changes to support vitellogenesis. In published transcriptomic approaches, hundreds of genes were reported to be overexpressed in laying hens and functional gene annotation using gene ontology tools have essentially revealed an enrichment in lipid and protein metabolisms. We reanalyzed some data from a previously published article comparing 38-week old versus 10-week old hens to give a more integrative view of the functions stimulated in the liver at sexual maturity and to move beyond current physiological knowledge. Functions were defined based on information available in Uniprot database and published literature.ResultsOf the 516 genes previously shown to be overexpressed in the liver of laying hens, 475 were intracellular (1.23–50.72 fold changes), while only 36 were predicted to be secreted (1.35–66.93 fold changes) and 5 had no related information on their cellular location. Besides lipogenesis and protein metabolism, we demonstrated that the liver of laying hens overexpresses several clock genes (which supports the circadian control of liver metabolic functions) and was likely to be involved in a liver/brain/liver circuit (neurotransmitter transport), in thyroid and steroid hormones metabolisms. Many genes were associated with anatomical structure development, organ homeostasis but also regulation of blood pressure. As expected, several secreted proteins are incorporated in yolky follicles but we also evidenced that some proteins are likely participating in fertilization (ZP1, MFGE8, LINC00954, OVOCH1) and in thyroid hormone maturation (CPQ). We also proposed that secreted proteins (PHOSPHO1, FGF23, BMP7 but also vitamin-binding proteins) may contribute to the development of peripheral organs including the formation of medullar bones to provide labile calcium for eggshell formation. Thirteen genes are uniquely found in chicken/bird but not in human species, which strengthens that some of these genes may be specifically related to avian reproduction.ConclusionsThis study gives additional hypotheses on some molecular actors and mechanisms that are involved in basic physiological function of the liver at sexual maturity of hen. It also revealed some additional functions that accompany reproductive capacities of laying hens, and that are usually underestimated when using classical gene ontology approaches.

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The Role of Hormones in the Regulation of Bone Turnover and Eggshell Calcification

Dacke

Sugiyama

2015

Sturkie's Avian Physiology

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Evidence for the expression of estrogen receptors in osteogenic cells isolated from hen medullary bone

Cited by 4 publications

References 53 publications

Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica)

Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica)

Integrative analysis of transcriptomic data related to the liver of laying hens: from physiological basics to newly identified functions

The Role of Hormones in the Regulation of Bone Turnover and Eggshell Calcification

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