Evidence for the in vivo existence and mobilisation of myeloid angiogenic cells and pericyte‐like cells in wound patients after skin grafting

Uusitalo‐Kylmälä, Liina; Joensuu, Katriina; Hietanen, Kristiina; Paloneva, Juha; Heino, Terhi J.

doi:10.1111/wrr.13047

Cited by 2 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pericytes (PCs), also known as mural or Rouget cells, are essential for wound angiogenesis [1,2]. These cells are found on the outer surface of the vasculature.…”

Section: Introductionmentioning

confidence: 99%

Tube Formation Capability and Chemotaxis of Skin Pericytes

Cui,

Lin,

Zhao

et al. 2024

Discovery Medicine

View full text Add to dashboard Cite

Background: Pericytes (PCs), the critical components of vessels, are implicated in wound repair. This study aimed to explore the roles of PCs in wound healing and angiogenesis. Methods: Skin PCs and human dermal microvascular endothelial cells (HDMECs) were isolated from patients' upper eyelid skin. Immunofluorescence staining was used to characterize the morphology of PCs. Tube formation and transwell chemotaxis assays were performed to explore PC's tube-forming capability and chemotaxis. Finally, we investigated the effects of PCs and endothelial cells on wound repair using skin wound of a rat model. Results: Skin PCs exhibited a double-protrusion structure and characteristic antigen expression of neural/glial antigen 2 (NG2) + /platelet-derived growth factor receptor-β (PDGFR-β) + /alpha-smooth muscle actin (α-SMA) + /CD31 − . Skin PCs could directly form lumen-like structures in a two dimensional (2D) culture environment, and mild hypoxia and starvation promoted the lumen-like structure formation. Furthermore, skin PCs quickly formed more stable lumen-like structures than HDMECs in matrigel, and they recruited HDMECs in a three dimensional (3D) culture environment. Transwell chemotaxis assay showed that PCs and HDMECs were chemotactic to each other. PCs could develop lumen-like structures in the skin wounds of rat models. The number of PCs mounted in wounded skin was compared to normal skin. The ratio of PCs to endothelial cells gradually increased after skin injury and reached its maximum on the 3rd day. Conclusions: Skin PCs have an excellent tube-forming capability and chemotaxis to endothelial cells. PCs might promote wound repair by recruiting endothelial cells.

show abstract

“…Pericytes (PCs), also known as mural or Rouget cells, are essential for wound angiogenesis [1,2]. These cells are found on the outer surface of the vasculature.…”

Section: Introductionmentioning

confidence: 99%

Tube Formation Capability and Chemotaxis of Skin Pericytes

Cui,

Lin,

Zhao

et al. 2024

Discovery Medicine

View full text Add to dashboard Cite

show abstract

Evidence for the in vivo existence and mobilisation of myeloid angiogenic cells and pericyte‐like cells in wound patients after skin grafting

Uusitalo‐Kylmälä

Joensuu

Hietanen

et al. 2022

Wound Repair Regeneration

View full text Add to dashboard Cite

Myeloid angiogenic cells (MACs) and pericyte-like cells, derived from peripheral blood mononuclear cells (MNCs) by in vitro culturing, are suggested as relevant cell types for angiogenesis and tissue repair. However, the in vivo existence and relevance of these cells has so far remained unknown. Our aim was thus to study, if MACs and pericyte-like cells exist in circulation during the wound healing of skin graft patients, and to evaluate the cellular features of wound repair. MNCs were isolated from blood samples of healthy controls (n = 4) and patients with a traumatic full thickness skin defect (n = 4) before skin grafting and on postoperative days 1 and 6. The numbers of circulating CD14 +-CD45 + CD31 + CD34 À MACs and CD14 + CD45 + NG2 + pericyte-like cells were assessed by flow cytometry, and gene expression of various pro-angiogenic factors was analysed by qPCR. Wound bed biopsies were taken on postoperative days 6 and 14, and MAC (CD31, CD14 and CD45) and pericyte-related markers (NG2 and PDGFRβ) were histologically studied. MACs and pericyte-like cells were detected in both healthy controls and in patients. Before reconstruction, on average 18% of all circulating MNCs represented MACs and 2% pericyte-like cells in wound patients. Number of MACs significantly increased 1.1À1.7-fold in all patients 1 day after skin grafting (p < 0.01). In addition, histological analysis demonstrated effective vascularization of skin grafts, as well as presence of pericytes, and CD14 and CD45 expressing myeloid cells during wound healing. In conclusion, our data shows, for the first time, the presence and mobilisation of MACs and pericyte-like cells in human circulation.

show abstract

Evidence for the in vivo existence and mobilisation of myeloid angiogenic cells and pericyte‐like cells in wound patients after skin grafting

Cited by 2 publications

References 36 publications

Tube Formation Capability and Chemotaxis of Skin Pericytes

Tube Formation Capability and Chemotaxis of Skin Pericytes

Evidence for the in vivo existence and mobilisation of myeloid angiogenic cells and pericyte‐like cells in wound patients after skin grafting

Contact Info

Product

Resources

About