Evidence for the Insulin-Directed Specificity of Rabbit Anti-Insulin Serum 1

Arquilla, Edward R.; Stavitsky, Abram B.

doi:10.1172/jci103298

Cited by 45 publications

(19 citation statements)

References 11 publications

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“…The insulin antibodies were found in the gamma globulin fraction of the rabbit serum.5 An anamnestic response to a single injection of insulin was elicited in rabbits which were previously immunized. In the next paper (16) data are presented concerning the serological cross-reactivity of insulins from various species, the neutralization of the physiological effects of insulin, and the specificity of the insulin antibodies produced in the rabbit.…”

Section: Discussionmentioning

confidence: 99%

“…Serum from insulin-resistant patients contained greatly increased amounts of these substances compared to normal, and non-resistant diabetics. We have evidence (16) The biological methods do not measure insulin itself, but only its effects. Therefore, these assays are useful in measuring insulin in samples which do not contain any substances that affect glucose metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…In the following paper (16) evidence is presented for the specificity of the rabbit antibodies for insulin itself, and for the immunological similarity of insulins from various species. Therefore, the hemolytic method employing rabbit antiserum to beef insulin is probably applicable to the measurement of insulin from various species.…”

Section: Discussionmentioning

confidence: 99%

“…No corrections for this non-specific hemolysis were made in this study. However, it was subsequently learned that the non-specific hemolysis could be reduced considerably if the guinea pig serum was previously absorbed with sheep erythrocytes, as described in the following paper (16). t R-10 was tested both 8 and 19 days after the last injection-on both of these occasions the serum titre was 0.…”

Section: Materials 2 and Methodsmentioning

confidence: 99%

“…Methods are also presented for the assay of microgram quantities of insulin in pure solutions. Evidence that rabbit anti-insulin serum is specific for insulin, and that there is a high degree of serological cross-reactivity among insulins from various species is presented in the next paper (16).…”

mentioning

confidence: 93%

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The Production and Identification of Antibodies to Insulin and Their Use in Assaying Insulin 1

Arquilla¹,

Stavitsky²

1956

J. Clin. Invest.

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124

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A specific method for the detection of small quantities of insulin in body tissues and fluids might help to resolve some of the problems connected with the mechanism of action and hormonal interrelationships of insulin. The biological assay methods for small quantities of insulin (2-4) are based upon the effect of insulin on glucose metabolism. Although very sensitive, these methods have several disadvantages. They are technically difficult, are dependent upon the physiological effect of insulin, therefore, subject to considerable biological variation, and consequently may not be specific for insulin per se. A method specific for insulin and independent of biological variation would, therefore, be highly desirable.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Materials 2 and Methodsmentioning

confidence: 99%

mentioning

confidence: 93%

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