Evidence for the Involvement of Annexin 6 in the Trafficking between the Endocytic Compartment and Lysosomes

Pons, Mónica; Grewal, Thomas; Rius, E.; Schnitgerhans, Tino; Jäckle, Stefan; Enrich, Carlos

doi:10.1006/excr.2001.5268

Cited by 47 publications

(62 citation statements)

References 60 publications

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“…Therefore we initially compared the localization of SNAP23 and syntaxin-4 in CHO wild-type (CHOwt) and the well-characterized CHO-A6 cell line (Grewal et al , 2000, 2005; Pons et al , 2001; Rentero et al , 2006; Cubells et al , 2007, 2008). CHOwt cells express low amounts of AnxA6, whereas AnxA6 levels in CHO-A6 cells are similar to AnxA6 expression levels observed in other commonly used cell lines, such as HeLa and NRK (Cubells et al , 2007).…”

Section: Resultsmentioning

confidence: 99%

“…The generation of the stable AnxA6-overexpressing CHO cell line (CHO-A6) and A431 cell line (A431-A6) has been described in detail (Grewal et al , 2000, 2005; Pons et al , 2001; Cubells et al , 2007; Vila de Muga et al , 2009). To generate AnxA6-overexpressing HuH7, we transfected HuH7 cells with pcDNAanx6 (Grewal et al , 2000) and selected in the presence of 1 mg/ml G418.…”

Section: Methodsmentioning

confidence: 99%

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Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function

Reverter¹,

Rentero²,

Muga³

et al. 2011

Molecular Biology of the Cell

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This study shows that impaired cholesterol egress from late endosomes in cells with high annexin A6 levels is associated with altered soluble N-ethylmaleimide–sensitive fusion protein 23 (SNAP23) and syntaxin-4 cellular distribution and assembly and accumulation in Golgi membranes. This correlates with reduced secretion of cargo along the constitutive and SNAP23/syntaxin-4–dependent secretory pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function

Reverter¹,

Rentero²,

Muga³

et al. 2011

Molecular Biology of the Cell

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“…Annexin VI has been implicated as an important regulatory component of clathrin-mediated endocytosis (15)(16)(17)28), although the primary significance of the protein in this process remains unclear (29). Lin et al (28) used an in vitro system to demonstrate that annexin VI is required for the Ca 2ϩ -and ATP-dependent budding of clathrin-coated pits from membranes.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent studies in intact fibroblasts indicated that annexin VI functions by directing the remodeling of membranebound spectrin during vesicle budding (15). In addition to modulating endocytosis at the plasma membrane, annexin VI was shown to be present in a late endocytic compartment of epithelial cells (30 -34), where it is thought to direct the delivery of endosomal vesicles to lysosomes (16,17). Interestingly, in smooth muscle, annexin VI plays a dynamic role in regulating reversible interactions between actin-cytoskeletal components and the caveolar fraction of the sarcolemma during muscle contraction (35,36).…”

Section: Discussionmentioning

confidence: 99%

“…The present study describes the purification of the 70-kDa CRHSP-28-binding protein as annexin VI and further demonstrates a colocalization of these molecules in the apical cytoplasm of acinar cells. Based on recent studies establishing a role for annexin VI in endocytic trafficking (15)(16)(17), it is proposed that CRHSP-28 may function at multiple steps in acinar cell membrane trafficking involving digestive enzyme secretion and zymogen granule membrane retrieval from the apical plasma membrane.…”

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confidence: 99%

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Identification of Annexin VI as a Ca2+-sensitive CRHSP-28-binding Protein in Pancreatic Acinar Cells

Thomas

Kaspar

Taft

et al. 2002

Journal of Biological Chemistry

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, indicating these molecules likely interact under physiological conditions. Immunofluorescence microscopy confirmed a dual localization of CRHSP-28 and annexin VI, which appeared in a punctate pattern in the supranuclear and apical cytoplasm of acini. Stimulation of cells for 5 min with the secretagogue cholecystokinin enhanced the colocalization of CRHSP-28 and annexin VI within regions of acini immediately below the apical plasma membrane. Tissue fractionation revealed that CRHSP-28 is a peripheral membrane protein that is highly enriched in smooth microsomal fractions of pancreas. Further, the content of CRHSP-28 in microsomes was significantly reduced in pancreatic tissue obtained from rats that had been infused with a secretory dose of cholecystokinin for 40 min, demonstrating that secretagogue stimulation transiently alters the association of CRHSP-28 with membranes in cells. Collectively, the Ca 2؉ -dependent binding of CRHSP-28 and annexin VI, together with their colocalization in the apical cytoplasm, is consistent with a role for these molecules in acinar cell membrane trafficking events that are essential for digestive enzyme secretion.

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Molecular mechanisms involved in Ras inactivation: the annexin A6–p120GAP complex

Grewal

Enrich

2006

BioEssays

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In mammalian cells, a complex network of signaling pathways tightly regulates a variety of cellular processes, such as proliferation and differentiation. New insights from one of the most-important signaling cascades involved in oncogenesis, the Ras-Raf-MAPK pathway, suggest that the subcellular localisation and assembly of signaling modules of this pathway is crucial to control the biological response. This commonly requires membrane targeting events that are mediated by adaptor/scaffold proteins. Of particular interest is the translocation and complex formation of GTPase-activating proteins (GAPs), such as p120GAP, at the plasma membrane to inactivate Ras. Recent studies indicate that one member of the annexin family, annexin A6 acts as a targeting protein for p120GAP. This review discusses how annexin A6 modulates the involvement of negative regulators of the Ras-Raf-MAPK pathway contributing to Ras inactivation.

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Evidence for the Involvement of Annexin 6 in the Trafficking between the Endocytic Compartment and Lysosomes

Cited by 47 publications

References 60 publications

Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function

Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function

Identification of Annexin VI as a Ca2+-sensitive CRHSP-28-binding Protein in Pancreatic Acinar Cells

Molecular mechanisms involved in Ras inactivation: the annexin A6–p120GAP complex

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