Evidence for the nonparticipation of DNA in viral RNA synthesis

Reproduction of Paramyxoviruses

“…This was established by the ability of these viruses to replicate in the presence of actinomycin D (Kingsbury, 1962;Barry et al, 1962;Rott and Scholtissek, 1964;Choppin, 1965) and in the presence of inhibitors of DNA synthesis (Simon, 1961;Choppin, 1965). Paramyxoviruses were also found to be capable of replication in UV -irradiated cells (Barry, 1964;Rosenbergovit and Rosenberg, 1964).…”

DNA Multiplication as a Requirement for Expression of Floral Stimulus in Pharbitis nil

“…Seedlings of this strain respond to one single dark period of 16 hours by the subsequent initiation of flower buds (8). The original seeds were generously supplied by Prof. S. Imamura, Kyoto University, Kyoto, Japan.…”

“…This was further investigated by applying two other inhibitors to Pharbitis plumules: A, aminopterin (4-amino pteroylglutamic acid) which, like 5-FDU, is known to block the synthesis of thymiiidylic acid and hence DNA synthesis (e.g. 16) and B, colchicine which permits DNA multiplicatiOnl, but blocks cell (livision (table V) Gibberellic acid, a known stimulator of cell division in the subapical region (13) has been found in the course of the present experiments to be fully ineffective as an antidote for 5-FU inhibition. This is as would be expected if thymidylic acid is the limiting factor.…”

Viruses, Polymerases, and Cancer

“…Before I began to study how picornavirus RNA was made, it was already known from the work of Simon that picornavirus RNA synthesis was independent of DNA synthesis (5). Furthermore, studies with actinomycin D had shown that neither synthesis nor expression of cellular DNA was involved in viral RNA synthesis (6).…”