Evidence for the presence of NK1 and NK3 receptors on cholinergic neurones in the guinea-pig ileum

Legat, Franz J.; Althuber, Petra; Maier, Robert; Griesbacher, Thomas; Lembeck, Fred

doi:10.1016/0304-3940(96)12503-9

Cited by 11 publications

(7 citation statements)

References 9 publications

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“…The presence of the NK1r on excitatory circular muscle motor neurons is in accord with pharmacological observations that excitation of the circular muscle is mediated in part through stimulation of neuronal NK1 receptors (Maggi et al 1990). However, the longitudinal muscle is also contracted via NK1 agonists acting on neurons (Legat et al 1996), but excitatory longitudinal muscle motor neurons, which are calretinin-IR, did not have NK1r-IR. It is possible that the longitudinal muscle motor neurons have a receptor that is not revealed by the antiserum (as is suggested for neurons in the submucous plexus below), that activation of these neurons by tachykinins is indirect, or that acetylcholine overflows from another source to cause longitudinal muscle contraction.…”

Section: Myenteric Nerve Cellsmentioning

confidence: 98%

“…It is particularly pertinent to determine whether cholinergic neurons have the receptor because pharmacological studies in the small intestine suggest that NK1 receptors are located on excitatory motor neurons (Maggi et al 1990;Legat et al 1996). An effective antiserum that locates ChAT in enteric neurons of the guinea-pig has now been characterised (Mawe et al 1996;); this provides the opportunity to determine the chemical coding, and hence the functional identity, of all the populations of NK1r-IR neurons in the guinea-pig ileum.…”

Section: Introductionmentioning

confidence: 99%

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Identification of the populations of enteric neurons that have NK1 tachykinin receptors in the guinea-pig small intestine

Lomax

Bertrand

Furness

1998

Cell and Tissue Research

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Simultaneous immunofluorescence labelling was used to investigate the patterns of colocalisation of the NK1 tachykinin receptor with other neuronal markers, and hence determine the functional classes of neuron that bear the NK1 receptor in the guinea-pig ileum. In the myenteric plexus, 85% of NK1 receptor-immunoreactive (NK1r-IR) nerve cells had nitric oxide synthase (NOS) immunoreactivity and the remaining 15% were immunoreactive for choline acetyltransferase (ChAT). Of the latter group, about 50% were immunoreactive for both neuropeptide Y (NPY) and somatostatin (SOM), and had the morphologies of secretomotor neurons. Many of the remaining ChAT neurons were immunoreactive for calbindin or tachykinins (TK), but not both. These calbindin immunoreactive neurons had Dogiel type II morphology. No NK1r-IR nerve cells in the myenteric plexus had serotonin or calretinin immunoreactivity. In the submucosal ganglia, 84% of NK1r-IR nerve cells had neuropeptide Y immunoreactivity and 16% were immunoreactive for TK. It is concluded that NK1r-IR occurs in five classes of neuron; namely, in the majority of NOS-immunoreactive inhibitory motor neurons, in ChAT/TK-immunoreactive excitatory neurons to the circular muscle, in all ChAT/NPY/SOM-immunoreactive secretomotor neurons, in a small proportion of ChAT/calbindin myenteric neurons, and in about 50% of ChAT/TK submucosal neurons.

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Section: Myenteric Nerve Cellsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Identification of the populations of enteric neurons that have NK1 tachykinin receptors in the guinea-pig small intestine

Lomax

Bertrand

Furness

1998

Cell and Tissue Research

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“…Three types of tachykinin receptor have been described so far: the NK 1 and NK 2 subtypes are located on smooth muscle cells (where they mediate contraction) and neurones, 15–17 whereas NK 3 receptors are restricted to enteric neurones in several species 15 . In the guinea‐pig at least, both NK 1 and NK 3 receptors are found on ascending and descending neuronal pathways, where they mediate release of excitatory or inhibitory transmitters 15 , 18–21 . Several studies support the notion that tachykinin release is responsible for the atropine‐resistant neurogenic contraction induced by activation of 5‐HT 4 receptors 22 , 23 …”

Section: Introductionmentioning

confidence: 96%

Intestinal motor stimulation by the 5‐HT₄ receptor agonist ML10302: differential involvement of tachykininergic pathways in the canine small bowel and colon

Ponti

Crema

Moro

et al. 2001

Neurogastroenterology Motil

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5-Hydroxytryptamine (5-HT)4 receptor agonists stimulate gut motility through cholinergic pathways, although there are data suggesting that noncholinergic (tachykininergic) excitatory pathways may also be involved. Differences may exist between the small bowel and colon. Our aims were: (i) to compare the prokinetic effect exerted by the 5-HT4 receptor agonist ML10302 in the canine small bowel and colon in vivo; and (ii) to investigate the role of tachykininergic pathways in mediating this response. In fasting, conscious dogs, chronically fitted with electrodes and strain-gauge force transducers along the small bowel and colon, intravenous injection of ML10302 (35 microg kg-1) immediately stimulated spike activity and significantly increased propagated myoelectrical events at both intestinal levels. In the small bowel, the effects of ML10302 were unchanged by previous administration of the selective NK1 tachykinin receptor antagonist SR140333, the NK2 tachykinin receptor antagonist SR48968, or the NK3 tachykinin receptor antagonist SR142801. In the colon, all tachykinin receptor antagonists significantly inhibited stimulation of spike and mechanical activity by ML10302, without affecting ML10302-induced propagated myoelectrical events. Atropine (100 microg kg-1 i.v.) suppressed the stimulatory effect of ML10302 at both intestinal levels. In conclusion, the 5-HT4 receptor agonist ML10302 induced significant prokinesia both in the small bowel and colon through activation of cholinergic pathways. Tachykininergic pathways are not involved in the ML10302-induced prokinesia in the small bowel, but they play an important role in mediating the colonic motor response to ML10302.

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“…However, we have no explanation for this effect, as NK3 receptors have never been reported to be present on immune cells. Until now NK3 receptors in the digestive tract have been identified only on myenteric and submucosal neurons in the rat gastrointestinal tract,12 on cholinergic neurons of the myenteric plexus in the guinea pig ileum,47 and on intrinsic sensory neurons projecting into the mucosa in the rat ileum 13…”

Section: Discussionmentioning

confidence: 99%

Involvement of tachykinin receptors in sensitisation to cow’s milk proteins in guinea pigs

Fioramonti

Garcia‐Villar

Emonds‐Alt

et al. 1999

Gut

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Background-There is growing evidence for a pivotal role for tachykinins in gut neuroimmune interactions. Aims-To determine whether NK1, NK2, and NK3 tachykinin receptors are involved in milk protein induced allergic sensitisation. Methods-Eight groups of 12 DunkinHartley guinea pigs (250-300 g) were used. Four groups were sensitised to milk proteins for three weeks. During this period, these animals were injected intraperitoneally each day with NK1 (SR 140333; 0.3 mg/kg), NK2 (SR 48968; 5 mg/kg), or NK3 (SR 142801; 5 mg/kg) receptor antagonist or vehicle. The fifth group had water available instead of milk and was used as a non-sensitised control. The three other groups received the NK receptor antagonists for three weeks but were not sensitised to milk proteins. Results-Sensitised animals treated with NK1 and NK3 receptor antagonists had both lower IgE and IgG serum titres, evaluated by passive cutaneous anaphylaxis, and lower specific IgG serum titres, determined by enzyme linked immunosorbent assay (ELISA), than vehicle treated animals. Sensitisation induced an increase in intestinal mast cell number which was abolished by treatment with the NK1 receptor antagonist. Antigenic challenge-induced jejunal hypersecretion was also blocked by treatment with the NK1 receptor antagonist. Conclusion-In guinea pigs, NK1 and NK3 but not NK2 receptors are involved in sensitisation to cow's milk. However, NK1 but not NK3 receptor antagonists abolish both the hypermastocytosis induced by food allergy and the hypersecretion induced by antigenic challenge, suggesting diVerent roles for NK1 and NK3 receptors in the mechanisms of sensitisation to -lactoglobulin. (Gut 1999;44:497-503)

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Evidence for the presence of NK1 and NK3 receptors on cholinergic neurones in the guinea-pig ileum

Cited by 11 publications

References 9 publications

Identification of the populations of enteric neurons that have NK1 tachykinin receptors in the guinea-pig small intestine

Identification of the populations of enteric neurons that have NK1 tachykinin receptors in the guinea-pig small intestine

Intestinal motor stimulation by the 5‐HT₄ receptor agonist ML10302: differential involvement of tachykininergic pathways in the canine small bowel and colon

Involvement of tachykinin receptors in sensitisation to cow’s milk proteins in guinea pigs

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Evidence for the presence of NK1 and NK3 receptors on cholinergic neurones in the guinea-pig ileum

Cited by 11 publications

References 9 publications

Identification of the populations of enteric neurons that have NK1 tachykinin receptors in the guinea-pig small intestine

Identification of the populations of enteric neurons that have NK1 tachykinin receptors in the guinea-pig small intestine

Intestinal motor stimulation by the 5‐HT4 receptor agonist ML10302: differential involvement of tachykininergic pathways in the canine small bowel and colon

Involvement of tachykinin receptors in sensitisation to cow’s milk proteins in guinea pigs

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Intestinal motor stimulation by the 5‐HT₄ receptor agonist ML10302: differential involvement of tachykininergic pathways in the canine small bowel and colon