Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in Larimichthys polyactis spermiogenesis

Wang, Jingqian; Gao, Xinming; Zheng, Xuebin; Du, Chang; Hou, Cong-Cong; Xie, Qingping; Liu, Bao; Liu, Feng; Jin, Songqing; Zhu, Junquan

doi:10.1007/s00343-020-0304-2

Cited by 1 publication

(5 citation statements)

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“…This particular interaction reflects the uniqueness of the KIF17b mediated transport of ACT [ 27 ]. In the present study, the predicted Pe -KIF17 protein had the same structural features as previously reported KIF17 [ 17 , 18 ]. Multiple sequence comparisons with KIF17 amino acid sequences from other species showed that their motor domains were highly conserved and contained microtubule-binding sites and ATP hydrolysis sites.…”

Section: Discussionsupporting

confidence: 85%

“…This coincides with the high expression of kif17 mRNA in stage IV of L. polyactis and L. crocea spermiogenesis. The testes of both contain numerous spermatids [ 17 , 18 , 34 ], which suggests the involvement in spermiogenesis, due to the high expression of KIF17 in the testes of L. crocea and L. polyactis [ 17 , 18 ]. Corresponding findings regarding the expression of kif17 mRNA revealed the highest expression in the CF, where spermatids underwent morphological transformation into sperm throughout spermiogenesis, in contrast to other tissues, during July of the breeding season.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have found that KIF17b and Spatial also exist in the manchette region and are continuously expressed in the sperm tails [ 19 ] and that KIF17b is expressed throughout spermatogenesis persists in the sperm tail [ 20 , 38 ]. Furthermore, in L. polyactis and L. crocea , KIF17 is involved in spermatid remodeling, including spermatid nucleus reshaping and sperm tail formation, through perinuclear microtubules during spermiogenesis [ 17 , 18 ]. Based on these findings, a model involving Pe -KIF17 in spermatid remodeling can be proposed ( Figure 9 A1,A2).…”

Section: Discussionmentioning

confidence: 99%

“…KIF17 is involved in spermiogenesis via two mechanisms. In the first mechanism, KIF17 operates in a microtubule-dependent manner, similar to other kinesins [ 17 , 18 ]. In mice, KIF17 (designated KIF17b in the testes) co-localized with Spatial in the manchette structure, where the sperm nucleus is slightly elongated in shape than a sphere.…”

Section: Introductionmentioning

confidence: 99%

“…As chromatin thickens, the manchette structure moves to one side of the nucleus and continues to be located in the principal piece of the sperm tail, suggesting that KIF17b may be involved in spermatid nucleus reshaping via the manchette [ 19 ]. In Larimichthys polyactis and Larimichthys crocea , KIF17 is involved in spermatid remodeling, including spermatid nucleus reshaping and tail formation through perinuclear microtubules during spermiogenesis [ 17 , 18 ]. Studies on vertebrates have shown that KIF17 and the manchette are involved in spermiogenesis; however, related studies in mollusks are yet to be conducted.…”

Section: Introductionmentioning

confidence: 99%

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Expression Dynamics Indicate Potential Roles of KIF17 for Nuclear Reshaping and Tail Formation during Spermiogenesis in Phascolosoma esculenta

Pan,

Wang,

Gao

et al. 2023

IJMS

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Kinesin family member17 (KIF17), a homologous dimer of the kinesin-2 protein family, has important microtubule-dependent and -independent roles in spermiogenesis. Little is known about KIF17 in the mollusk, Phascolosoma esculenta, a newly developed mariculture species in China. Here, we cloned the open reading frame of Pe-kif17 and its related gene, Pe-act, and performed bioinformatics analysis on both. Pe-KIF17 and Pe-ACT are structurally conserved, indicating that they may be functionally conserved. The expression pattern of kif17/act mRNA performed during spermiogenesis revealed their expression in diverse tissues, with the highest expression level in the coelomic fluid of P. esculenta. The expressions of Pe-kif17 and Pe-act mRNA were relatively high during the breeding season (July–September), suggesting that Pe-KIF17/ACT may be involved in spermatogenesis, particularly during spermiogenesis. Further analysis of Pe-kif17 mRNA via fluorescence in situ hybridization revealed the continuous expression of this mRNA during spermiogenesis, suggesting potential functions in this process. Immunofluorescence showed that Pe-KIF17 co-localized with α-tubulin and migrated from the perinuclear cytoplasm to one side of the spermatid, forming the sperm tail. Pe-KIF17 and Pe-ACT also colocalized. KIF17 may participate in spermiogenesis of P. esculenta, particularly in nuclear reshaping and tail formation by interacting with microtubule structures similar to the manchette. Moreover, Pe-KIF17 with Pe-ACT is also involved in nuclear reshaping and tail formation in the absence of microtubules. This study provides evidence for the role of KIF17 during spermiogenesis and provides theoretical data for studies of the reproductive biology of P. esculenta. These findings are important for spermatogenesis in mollusks.

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Section: Discussionsupporting

confidence: 85%