Evidence for Two Independent Lineages of Shiitake of the Americas (Lentinula boryana) Based on rDNA and β-Tubulin Gene Sequences

Pleurotus eryngii species complex: Sequence analysis and phylogeny based on partial EF1α and RPB2 genes

“…and Grifola spp. is driven by isolation of populations by allopatry (geographic separation) (Vilgalys 1991;Thon and Royse 1999;Isikhuemhen et al 2000;James et al 2001;Shen et al 2002;Zervakis et al 2004). In the case of P. tuberregium, for example, it was estimated that divergence of the African and Australasian-Pacific populations occurred as consequence of the separation of the land 150 million years ago (Isikhuemhen et al 2000).…”

Molecular evolution of Agaricus species based on ITS and LSU rDNA sequences

“…Use of ribosomal DNA (rDNA) sequences to infer phylogenetic relationships among agaric fungi now is widely exploited (for example, see BRUNS, WHITE & TAYLOR 1991, BUNYARD, NICHOLSON & ROYSE 1996, HIBBETT et al 1995, HIBBETT, GRIMALDI & DONOGHUE 1997b, HOPPLE & VILGA-LYS 1999, HILLIS & DIXON 1991, JOHNSON & VILGALYS 1999, LUTZONI & VILGALYS 1995, MONCALVO, WANG & HSEU 1995, MONCALVO, DREHMEL & VILGALYS 2000a, MONCALVO et al 2000b, NICHOLSON 1995, PINE, HIBBETT & DONOGHUE 1999, THON & ROYSE 1999. Use of ribosomal DNA (rDNA) sequences to infer phylogenetic relationships among agaric fungi now is widely exploited (for example, see BRUNS, WHITE & TAYLOR 1991, BUNYARD, NICHOLSON & ROYSE 1996, HIBBETT et al 1995, HIBBETT, GRIMALDI & DONOGHUE 1997b, HOPPLE & VILGA-LYS 1999, HILLIS & DIXON 1991, JOHNSON & VILGALYS 1999, LUTZONI & VILGALYS 1995, MONCALVO, WANG & HSEU 1995, MONCALVO, DREHMEL & VILGALYS 2000a, MONCALVO et al 2000b, NICHOLSON 1995, PINE, HIBBETT & DONOGHUE 1999, THON & ROYSE 1999.…”

DNA Sequencing Analysis of ITS and 28S rRNA of Poria cocos

“…Other fungal crude drugs that belong to the Polyporaceae, Ganoderma lucidum, and Polyporus umbellatus, were reported to have various substitutions in ITS 1 and 2. [9][10][11][12] Although the nucleotide lengths of ITS 1 and 2 of Poria cocos samples were remarkably longer than Ganoderma and Polyporus, they preserved almost identical sequences in these regions. Some of the Poria cocos samples possessed combined sequences, their sequences were combinations of the common sequences and those with insertions of 1-3 bps at single sites.…”

“…Altogether, the nucleotide lengths of ITS 1 and 2 of these Poria cocos samples were substantially longer in comparison with other fungus crude drugs, such as Ganoderma lucidum and Polyporu umbellatus; the lengths of their ITS 1 and 2 sequences were reported within the ranges of 190-220 bps and 170-200 bps, respectively. [9][10][11][12] When the PCR products amplified from the ITS 1 region using primer set a and d of the Poria cocos samples and those of Ganoderma lucidum and Polyporus umbellatus were compared, they could be clearly distinguished from each other by agarose gel electrophoresis (Fig. 1).…”