Evidence for uncoupled synthesis of viral RNA and viral capsids

On the Morphology and Development of Insect Cytoplasmic-Polyhedrosis Virus Particles

“…6) must remain empty. Support for such a conclusion is obtained from recent studies by Halperen et al (9), who found that synthesis of empty capsids continued in ECHO-12 virus-infected cells despite the arrest of synthesis of complete virus particles.…”

Chemotherapy of Viral Infections

“…First, it is to be assumed to be due to the limited cross-resistance between HBB and guanidine, whereby resistant mutants do have a reduced probability of replication. Both are inhibitors of picornavirus replication, both inhibit the synthesis of viral RNA and functional viral RNA polymerase, and both apparently do not directly affect viral protein syntheses (HALPEREN et al 1964). This is clearly shown in Table 1.…”

“…This straightforward interpretation, how- HALPEREN et al 1964) ever, raises difficulties. With inhibition of synthesis of the RNA polymerase the replication of viral RNA also comes to a halt which, in turn, finally leads to inhibition of synthesis of viral capsid polypeptides.…”

“…It could be shown that the increase in virus hemagglutinin for a period of approximately 1-1.5 h after addition of HBB (Fig. (EGGERS and TAMM 1962a) shown that the empty capsids produced after addition of HBB were comprised of polypeptides, synthesized after addition of the compound (HALPEREN et al 1964). 7;EGGERS and TAMM 1962a;HALPEREN et al 1964), a result in complete agreement with the rapid stop of infectious virus after addition of HBB.…”

Bovine Enterovirus CPE at Different Multiplicities of Infection in the Absence of Viral RNA Synthesis

“…Inhibiting viral pro tein synthesis would not be satisfactory be cause viral proteins are needed to catalyze the synthesis of DS RNA. HBB [2-(a-hydroxybenzyl)-benzimidazole] completely inhibits vi ral RNA synthesis in cells infected with certain picornaviruses while only partially inhibiting the synthesis of viral protein [16,17]. To de termine whether BEV was sensitive to HBB, we infected 25-ml samples containing 1 x 106 HEp-2 cells/ml with BEV at an MOI of 20 PFU/cell either with or without 100 p.g/ml HBB.…”