Evidence of antidiabetic activity of Spirulina fusiformis against streptozotocin-induced diabetic Wistar albino rats

Simon, Jerine Peter; Baskaran, Udhaya Lavinya; Shallauddin, Kadar Basha; Ramalingam, Giridharan; Sabina, Evan Prince

doi:10.1007/s13205-018-1156-8

Cited by 21 publications

(21 citation statements)

References 39 publications

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“…The presence of methoxy group in aromatic ring of compound (5) was supported by the appearance of sharp singlet at δ 3.81 (ppm) in the 1 H-NMR spectrum. 13 C-NMR spectrum confirmed the addition of carbon at δ (ppm) 60.8 that showed addition of methoxy group in compound (5). Furthermore, the molecular weight of compound (5) was found to be appropriate with the target structures.…”

Section: Compound (3)mentioning

confidence: 73%

“…1 H NMR (proton NMR) was identified using JEOL JNM-ECP (500 Hz) in CDCl 3 as solvent. 13 C NMR (carbon NMR) was identified using JEOL JNM-ECP (125 Hz) in CDCl 3 as solvent. The measurement results are known from the difference of the chemical shifts which expressed in ppm and coupling constants are measured in Hertz (Hz).…”

Section: Synthesis Reactionmentioning

confidence: 99%

“…The molecular docking process predicts ligand confirmation and orientation on an active site that is the target of the ligand and has an important role in drug design. 13 According Lipinski, the absorption and permeability of compounds with molecular weight above 500 possibilities is worse. While log P is not more than 5, where log P expresses solubility of the compound in octanol/water, for the amount of H acceptor no more than 10 and H donor no more than 5.…”

Section: In Silico Studymentioning

confidence: 99%

“…Then we had to hydrolyse the previous product, using aqueous lithium hydroxide, and give monomethoxy gallic acid as aproduct. The structures of product synthesized were identified by 1 H NMR, 13 C NMR, and mass spectrometry. The spectroscopic data of title compounds (3-5) are presented as follows:…”

Section: Synthesis Of the Desired Compounds (4-5)mentioning

confidence: 99%

“…The chemical structures of these compounds were identified by 1 H NMR, 13 C NMR, and mass spectrometry.…”

Section: Compound (3)mentioning

confidence: 99%

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In Silico Study, Synthesis, and Cytotoxic Activity of Esterification of Eugenol and Gallic Acid Against HT-29 Cell Line

Fadilah¹,

Yanuar²,

Arsianti³

et al. 2017

Orient. J. Chem

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Eugenol and gallic acid have cytotoxic effects in HT-29 colon cancer cells. In this study, we examined cytotoxic effect of esterification of eugenol and gallic acid. Before synthesized process, in silico docking was conduct to eugenol, gallic acid and its esterification products against Bcl-2 protein. After in silico docking, Eugenol and gallic acid were allowed to react by Mitsunobu Reaction to obtain compound (4-5). Proton and carbon NMR spectroscopy, also mass spectra (GCMS) were used to characterize of the structure from products. The products from esterification were tested by cytotoxic activity and showed by IC 50 with navitoclax as positive control. In silico docking result showed compound (5) had the lowest Gibb's Energy. Compound 4 and 5 were successfully synthesized and characterized by spectrometer 1 H-NMR, 13 C-NMR, and mass spectrometry. In silico docking result related to cytotoxic assay against HT-29 cell line. By replacing hydroxyl group of gallic acid with methoxy groups had increased the lipophilicity and the cytotoxicity. The greatest inhibitor against HT-29 cell line was compounds (5) with IC 50 values of 22.81 µg/ml. The synthesized esterification product, compound (5), had greater cytotoxic activity than eugenol and gallic acid against colon cancer cell line, HT-29. Thus, the esterification products should be considered as a promising candidate for cancer colon drug.

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Section: Compound (3)mentioning

confidence: 73%

Section: Synthesis Reactionmentioning

confidence: 99%

Section: In Silico Studymentioning

confidence: 99%

Section: Synthesis Of the Desired Compounds (4-5)mentioning

confidence: 99%

“…The chemical structures of these compounds were identified by 1 H NMR, 13 C NMR, and mass spectrometry.…”

Section: Compound (3)mentioning

confidence: 99%

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In Silico Study, Synthesis, and Cytotoxic Activity of Esterification of Eugenol and Gallic Acid Against HT-29 Cell Line

Fadilah¹,

Yanuar²,

Arsianti³

et al. 2017

Orient. J. Chem

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Therapeutic efficacy of spirulina against ovalbumin and cigarette smoke‐induced asthma‐specific stress biomarkers in Sprague–Dawley rats

Riaz

Butt

Sharif

et al. 2022

Food Science & Nutrition

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Due to the high prevalence of allergies and asthma, awareness about allergens and therapeutic use of functional foods and nutraceuticals have gained immense attention. Spirulina powder is being used as health‐boosting and antioxidant agent against several ailments owing to its unique nutritional profile. Considering its antioxidant role, the current study was focused on exploring therapeutic role of spirulina against stress biomarkers in asthmatic model. To assess the therapeutic efficacy of spirulina against allergic asthma‐specific oxidative stress biomarkers, a model feed trial was conducted and rats were divided into four groups ( n = 10). G 0–I (negative control), G 0–II (positive control), whereas G I (spirulina) and G 2 (salbutamol) served as treatment groups. Salbutamol is a chemical compound which is used in several antiallergic medicines because it works as bronchodilator. G 2 group was given salbutamol for comparison of results. For asthma induction, rats were given intraperitoneal injection of ovalbumin on 7th, 14th, and 21st day. Treatment groups were given spirulina powder (500 mg/kg body weight) and salbutamol (1 mg/kg), respectively, after the induction of asthma. All three asthmatic groups were also exposed to cigarette smoke daily along with respective treatment for 4 weeks. Asthma induction caused an increase in total cell count in bronchioalveolar fluid (BALF), while spirulina treatment reduced total cells in BALF by 33.50% and salbutamol by 41.7%. Level of interleukins (IL) like IL‐4 decreased by 33.32% & 48.56% in G 1 and G 2 . Similarly, IL‐5 and IL‐13 levels reduced by 40.9% & 49.9% and 18.62% & 38.02%, respectively, in G 1 and G 2 . Serum levels of Immunoglobin‐E (Ig‐E) declined by 29.70% and 52.82%, while histamine levels were 26.23% & 45.58% less at the end of study in comparison to positive control. Moreover, histological analysis of lung tissue revealed that both spirulina and salbutamol effectively reduced ovalbumin and cigarette smoke‐induced moderate to severe necrosis, architectural changes, and congestion. It was concluded that salbutamol showed better results however, spirulina also effectively reduced mild to moderate allergic symptoms in dose‐dependent manner. Nutraceutical and functional foods are considered helpful in mitigating oxidative stress‐mediated health problems. Spirulina has its unique nutritional profile including phycobiliproteins, phytochemicals, and antioxidant vitamins which make it useful against several ailments. Considering its antioxidant role, current study was focused on exploring therapeutic efficacy of spirulina against stress biomarkers in asthmatic model. Outcomes of present research also demonstrated beneficial effect of spirulina in modulating allergic symptoms. In this regard, ancient concept of ...

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Antidiabetic activity of standardized dried tubers extract of Aconitum napellus in streptozotocin‐induced diabetic rats

et al. 2020

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India has got rich cultural inheritage in the forms of Ayurveda texts which are a rich and ample source of herbs, shrubs, trees and affluent in medicinally active phytoconstituents. Aconitum napellus is used for the cure of many ailments including rheumatoid arthritis, sciatica and gout. The present work attempts to evaluate the physicochemical and preliminary phytochemical studies on the tubers of Aconitum napellus along with its antidiabetic activity. The herbal standardization was carried out on the basis of organoleptic properties, physical characteristics and physicochemical properties. The body weight of ACON-I (1.25 mg/kg) and ACON-II (2.5 mg/kg) was recorded as 190.40 and 209.40 g, respectively, compared with 163.00 g in diabetic rats at day 28. The body weight of ACON-I and ACON-II was significantly increased compared with diabetic rats (p < 0.01). However, the body weight of ACON-I and ACON-II was decreased significantly (p < 0.01) compared with normal group (222.60 g). The blood glucose levels of diabetic rats and ACON-I group were recorded as 277.800 and 152.400 mg/dl, respectively, compared with 83.600 mg/dl in normal rats (p < 0.01). However, the HbA1c levels of diabetic rats and ACON-I group were recorded as 11.306 and 6.936% Hb, respectively, compared with 4.539% Hb in normal rats. The glucose and HbA1c levels of diabetic and ACON-I groups were significant compared with normal group (p < 0.01). The results of antidiabetic activity showed that the plant can be used as a potent source for the treatment of diabetes and its complications. The results of this work provided the referential information for the identification and standardization of Aconitum napellus along with its role as a hypoglycemic agent.

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Evidence of antidiabetic activity of Spirulina fusiformis against streptozotocin-induced diabetic Wistar albino rats

Cited by 21 publications

References 39 publications

In Silico Study, Synthesis, and Cytotoxic Activity of Esterification of Eugenol and Gallic Acid Against HT-29 Cell Line

In Silico Study, Synthesis, and Cytotoxic Activity of Esterification of Eugenol and Gallic Acid Against HT-29 Cell Line

Therapeutic efficacy of spirulina against ovalbumin and cigarette smoke‐induced asthma‐specific stress biomarkers in Sprague–Dawley rats

Antidiabetic activity of standardized dried tubers extract of Aconitum napellus in streptozotocin‐induced diabetic rats

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