Evidence of early subchondral bone changes in the meniscectomized guinea pig. A densitometric study using dual-energy X-ray absorptiometry subregional analysis

Pastoureau, Philippe; Chomel, Agnès; Bonnet, Jacqueline

doi:10.1053/joca.1999.0241

Cited by 75 publications

(57 citation statements)

References 24 publications

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“…These changes were localized to the OAaffected region. With regard to the BMD of the Hartley strain guinea pig, Pastoureau et al [24] reported decrease of BMD one month after menisectomy followed by increase at 3 months postoperatively at the operated side of the femur. The early subchondral bone changes in their study correlate with our results.…”

Section: Discussionmentioning

confidence: 99%

Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy

Sato

Wada

Miyoshi

et al. 2004

Acta Histochem. Cytochem

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The aim of the present study was to investigate the quantity and quality of hydroxyapatite (HA) in the zone of calcified cartilage of articular cartilage and underlying subchondral bone by Fourier transform infrared microscopy (FTIRM). We hypothesized that the amount of HA deposited in osteoarthritis (OA) might differ from normal cartilage, and that such differences might be significant in the pathology of OA. The tibial epiphysis of the male Hartley strain for the spontaneous OA model and the control Strain 13 guinea pigs (12-month old) were examined. The mineral:matrix ratio was determined by integrating areas of u1, u3 bands at 900-1200 cm -1 to Amid 1 band at 1585-1725 cm -1 . The CO 3 :PO 4 ratio was determined by integrating areas of u2 carbonate band at 850-900 cm -1 to u1, u3 bands. In Hartley strain, the mean mineral:matrix ratio of the medial side was significantly lower than the lateral side at the zone of the calcified cartilage and subchondral bone. The mean CO 3 :PO 4 ratio was significantly higher in the medial side than the lateral one at the zone of the calcified cartilage and subchondral bone. In contrast, the results obtained from Strain 13 showed no significant differences between the two sides. Our results showed diminished deposition and increased maturation of HA in Hartley strain. These findings may correlate with the pathological process of knee OA, and indicate that FTIRM is a useful tool for determining the variability of HA in OA.

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Section: Discussionmentioning

confidence: 99%

Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy

Sato

Wada

Miyoshi

et al. 2004

Acta Histochem. Cytochem

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“…In a guinea pig model, bone densitometry evaluation following meniscectomy revealed typical variations in bone metabolism with early resorption of subchondral bone followed by increased bone density [18]. This concurs with findings from other animal models, such as the ACL dog and rat OA models, in which were observed, at an early stage of the disease process, increased subchondral bone resorption with trabecular thickness reduction and an increased number of osteoclasts, as well as increased production of catabolic factors including cathepsin K and matrix metalloproteinase (MMP)-13 [6][7][8][9].…”

Section: Correlation Between In Vivo Findings In Animals and Human Oamentioning

confidence: 99%

Targeting subchondral bone for treating osteoarthritis: what is the evidence?

Tat

Lajeunesse

Pelletier

et al. 2010

Best Practice & Research Clinical Rheumatology

157

121

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Over the past few decades, significant progress has been made with respect to new concepts about the pathogenesis of osteoarthritis (OA). This article summarises some of the knowledge we have today on the involvement of the subchondral bone in OA. It provides substantial evidence that changes in the metabolism of the subchondral bone are an integral part of the OA disease process and that these alterations are not merely secondary manifestations, but are part of a more active component of the disease. Thus, a strong rationale exists for therapeutic approaches that target subchondral bone resorption and/or formation, and data evaluating the drugs targeting bone remodelling raise the hope that new treatment options for OA may become available.

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“…Not many studies have investigated time-course relationships from before the onset of cartilage degeneration. The Hartley guinea pig spontaneously develops degenerative changes of cartilage that are similar to those of human OA in the knee joint, and it is used as an OA model (1,7,12,20,21,(26)(27)(28)(29)(30)(31)(32). However, unlike surgical OA models using non-surgically treated animals as controls, no control has been available for the Hartley guinea pig model, which is disadvantageous (31).…”

Section: Figure 4 Examples Of 3-dimensional Reconstruction Of Microfmentioning

confidence: 99%

“…Localization of the early lesions of OA has not been clarified, but many researchers have focused on articular cartilage (1,2) and have considered that changes in subchondral bone occur subsequent to the degeneration of articular cartilage. However, a low bone mineral density (BMD), particularly in the knee joint with OA (3,4), high bone turnover (5-7), and efficacy of bone resorption inhibitors for OA (8)(9)(10)(11)(12)(13)(14) have recently been reported, suggesting that subchondral bone plays an important role in the pathogenesis of knee OA.…”

mentioning

confidence: 99%

Role of subchondral bone in osteoarthritis development: A comparative study of two strains of guinea pigs with and without spontaneously occurring osteoarthritis

Muraoka¹,

Hagino²,

Okano³

et al. 2007

Arthritis & Rheumatism

109

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Objective. To evaluate the relationships among cartilage and subchondral bone before and after the onset of cartilage degeneration in the Hartley guinea pig model of spontaneous osteoarthritis (OA) as compared with those in Weiser-Maple guinea pigs, which do not develop OA.Methods. Mice from each strain were used at ages 2, 3, 5, and 8 months (n ‫؍‬ 7 at each time point). The region observed was the medial tibial plateau. Cartilage degeneration was evaluated histologically. Subchondral bone structure was evaluated based on subchondral bone plate thickness and subchondral cancellous bone trabecular parameters calculated from the microfocal computed tomography 3-dimensional reconstruction image. The bone mineral density (BMD) of the subchondral cancellous bone as well as levels of urinary N-telopeptide of type I collagen (NTX) and serum osteocalcin (OC) were measured.Results. In Hartley guinea pigs, the number of chondrocytes in the surface layer started to decrease at 3 months. At 8 months, fibrillation expanded to the radial zone. In Weiser-Maple guinea pigs, no cartilage degeneration was noted even at 8 months. Subchondral bone plate thickness was significantly lower in Hartley guinea pigs than in Weiser-Maple guinea pigs at 2 months. The subchondral bone had a rod-like and convex structure at 2 months in Hartley guinea pigs. BMD was significantly lower in Hartley guinea pigs than in Weiser-Maple guinea pigs at 2 months. The serum OC level was significantly higher in Hartley guinea pigs than in Weiser-Maple guinea pigs at 2 months and 3 months, whereas the urinary NTX level was significantly lower in Hartley guinea pigs at 3 months.Conclusion. Subchondral bone is fragile, and bone formation may be promoted in subchondral bone before the onset of cartilage degeneration in Hartley guinea pigs. Subchondral bone may be involved in the development of OA.Osteoarthritis (OA) is the most prevalent of joint diseases, and its pathology is characterized by the degeneration of articular cartilage, sclerosis of subchondral bone, and osteophyte formation. Localization of the early lesions of OA has not been clarified, but many researchers have focused on articular cartilage (1,2) and have considered that changes in subchondral bone occur subsequent to the degeneration of articular cartilage. However, a low bone mineral density (BMD), particularly in the knee joint with OA (3,4), high bone turnover (5-7), and efficacy of bone resorption inhibitors for OA (8)(9)(10)(11)(12)(13)(14) have recently been reported, suggesting that subchondral bone plays an important role in the pathogenesis of knee OA. Burr (15) reported that microdamage to subchondral bone by mechanical overload was repaired and led to bone sclerosis, by which bone turnover was enhanced and resulted in the deterioration of subchondral bone, suggesting the importance of subchondral bone and bone turnover in the progression of OA. Most of these clinical and experimental studies of subchondral bone were related to OA progression, but there have been fewer studies con...

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Evidence of early subchondral bone changes in the meniscectomized guinea pig. A densitometric study using dual-energy X-ray absorptiometry subregional analysis

Cited by 75 publications

References 24 publications

Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy

Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy

Targeting subchondral bone for treating osteoarthritis: what is the evidence?

Role of subchondral bone in osteoarthritis development: A comparative study of two strains of guinea pigs with and without spontaneously occurring osteoarthritis

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