Evidence of latent <scp>HPV</scp> infection in older Danish women with a previous history of cervical dysplasia

Hammer, Anne; Blaakær, Jan; Koning, Maurits N.C. de; Steiniche, Torben; Mejlgaard, Else; Svanholm, Hans; Roensbo, Mette T; Fuglsang, Katrine; Doorbar, John; Andersen, Rikke H.; Quint, Wim; Gravitt, Patti E.

doi:10.1111/aogs.14362

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…In contrast with other studies in Mexico and worldwide, 4 , 28 , 33 , 34 the first peak occurs at ages in the range 25–29 years, with a decline in the number of cases in middle-aged women (30–49 years); the second peak, observed after menopause, could be explained by the reactivation of a latent HR-HPV infection linked with a decline in the immune response. 35 , 36 Infection rates are strongly dependent on the number of sexual partners and a patient’s sexual behavior. Higher transmission rates result from the increase in the number of cases in young women, aged 20–25 years.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Risk Factors for High-Risk Human Papillomavirus Infection and Cervical Disorders: Baseline Findings From an Human Papillomavirus Cohort Study

Saldaña-Rodríguez,

Bahena-Román,

Delgado-Romero

et al. 2023

Cancer Control

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Background A persistent infection by high-risk human papillomavirus (HR-HPV) is a prerequisite for the development of cervical neoplasms; however, most studies have focused on risk factors associated with HPV-16 and HPV-18 only. Objectives We assessed the association of risk factors with the prevalence of HPV-16, HPV-18, and non-16/18 HR-HPV infection and with the occurrence of cervical lesions in the baseline of a cohort study of HPV persistence in a Mexican population. Methods Cross-sectional study within the baseline of a 5-year dynamic cohort study of HR-HPV persistence in women with an abnormal cytology study result from 2015 to 2021. HPV DNA was detected using the Anyplex II HPV 28 kit. Data on lifestyle, sociodemographic, and reproductive factors were assessed using bivariate and multivariate analyses to determine the association of risk factors with HR-HPV infection status and histopathologic diagnosis. Results A total of 373 women were included in the study. The overall prevalence of HR-HPV infection was 69.97%. The most prevalent HR-HPV genotypes, including single and multiple infections, were HPV-53 (13.4%), HPV-16 (11.8%), HPV-58 (10.9%), HPV-31 (10.9%), and HPV-66 (10.7%). We found 90 multiple HR-HPV infection patterns, all of them with α-6 and -9 species. Significant associations of multiple HPV-16 and non-16/18 HR-HPV infections were found with marital status, number of lifetime sexual partners, and smoking history. The most prevalent genotype in CIN1 and CIN2 patients was HPV-16. No association was found between biological plausibility risk factors and cervical lesions. Conclusions The risk factors for non-16/18 HR-HPV multiple infections are no different than those linked to HPV-16 multiple infections.

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Section: Discussionmentioning

confidence: 99%

Prevalence and Risk Factors for High-Risk Human Papillomavirus Infection and Cervical Disorders: Baseline Findings From an Human Papillomavirus Cohort Study

Saldaña-Rodríguez,

Bahena-Román,

Delgado-Romero

et al. 2023

Cancer Control

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“…Interestingly, our own work using RNAscope approaches has revealed evidence of basal and suprabasal HPV gene expression in biopsies that were negative following HPV typing on the cervical smear but positive in the whole tissue section. It is interesting that other studies using targeted research-based approaches and/or more sensitive tissue-based HPV typing methodologies have suggested that subclinical, immune controlled or latent infections may be more common than at first anticipated, particularly in women with a past history of HPV-associated cervical neoplasia [ 49 , 50 ].…”

Section: Defining Latency and Immune Control As Components Of The Pap...mentioning

confidence: 99%

The human Papillomavirus twilight zone – Latency, immune control and subclinical infection

Doorbar¹

2023

Tumour Virus Research

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“…Similarly, Molden and associates argue that the prognostic value of DNA testing for identifying severe dysplasia is limited, whereas HPV E6/E7 mRNA detection in combination with cytology is a more reliable prognostic indicator [ 8 , 9 , 10 ]. Additional studies highlight the occurrence of HPV DNA in cervical tissues without signs of active infection, pointing to a possible latent infection under immunologic control [ 11 ]. These insights support a nuanced screening approach that integrates DNA and mRNA testing to enhance our understanding of cervical cancer progression.…”

Section: Introductionmentioning

confidence: 99%

Quantification of HPV16 E7 Oncoproteins in Urine Specimens from Women with Cervical Intraepithelial Neoplasia

Makioka,

Inada,

Awano

et al. 2024

Microorganisms

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We present the validity of using an ultrasensitive enzyme-linked immunosorbent assay (ELISA) for quantifying high-risk human papillomavirus (HPV) 16 E7 oncoproteins in urine specimens as a noninvasive method of analyzing the oncogenic activity of HPV. Some reports claim that the oncogenic activity of HPV is a more relevant clinical indicator than the presence of HPV DNA for estimating malignant potential. In the present study, urine containing HPV16 and related types were selected by uniplex E6/E7 polymerase chain reaction and classified according to the pathologic diagnosis of cervical intraepithelial neoplasia (CIN) in cervical biopsy specimens. Our ultrasensitive ELISA was able to detect attomole levels of HPV16 E7 oncoproteins, and it detected HPV16-positive SiHa cells at >500 cells/mL without detecting HPV18-positive cells. Our ELISA results showed E7 oncoproteins in 80% (4/5) of urine specimens from women with HPV16-positive CIN1, 71% (5/7) of urine specimens from CIN2 patients, and 38% (3/8) of urine specimens from CIN3 patients. Some urine specimens with undetectable E7 oncoproteins were thought to be negative for live HPV 16-positive cells or in an inactivated state of infection. These results provide the basis for assessing oncogenic activity by quantifying E7 oncoproteins in patient urine.

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Evidence of latent HPV infection in older Danish women with a previous history of cervical dysplasia

Cited by 7 publications

References 31 publications

Prevalence and Risk Factors for High-Risk Human Papillomavirus Infection and Cervical Disorders: Baseline Findings From an Human Papillomavirus Cohort Study

Prevalence and Risk Factors for High-Risk Human Papillomavirus Infection and Cervical Disorders: Baseline Findings From an Human Papillomavirus Cohort Study

The human Papillomavirus twilight zone – Latency, immune control and subclinical infection

Quantification of HPV16 E7 Oncoproteins in Urine Specimens from Women with Cervical Intraepithelial Neoplasia

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