Evidence of porcine circovirus-like virus P1 in piglets with an unusual congenital tremor

Li, Wen; Mao, Aihua; Jiao, Fangfang; Zhang, D.; Xie, Jianping; He, Kongwang

doi:10.1111/tbed.12772

Cited by 15 publications

(15 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PMWS, Aujeszky’s disease, porcine reproductive and respiratory syndrome (PRRS), aflatoxicosis, classical and African swine fever. Since Sweden has active surveillance programs and are deemed free from for Aujeszky’s disease, porcine reproductive and respiratory syndrome (PRRS) as well as classical and African swine fever, and PMWS (PCV-2) not are supposed to cause congenital tremor [ 30 – 33 ] we speculate that APPV is a causative agent of congenital tremor in Swedish piglets as well as in other countries [ 34 – 36 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

2020

View full text Add to dashboard Cite

Background: Congenital tremor (CT) type A-II is a neurological disorder characterized by tremor of the head and body of newborn piglets. The suggested causative agent of the disease is the recently found atypical porcine pestivirus (APPV). The virus has been detected in piglets suffering from congenital tremor in central Europe, South and North America and in China but no studies has so far been performed in the Nordic countries. The overarching goal of this study was to investigate if APPV is present in the brain tissue of Swedish piglets suffering from congenital tremor. From June 2017-June 2018, 15 piglets from four Swedish farms with ongoing outbreaks of congenital tremor and 13 piglets with splay leg originating from four different farms, were investigated for presence of APPV RNA in brain tissue. Matched healthy control piglets (n = 8) were also investigated. Two APPV-specific RT-qPCR methods targeting the NS3 and NS5B region, respectively, were used. A retrospective study was performed on material from Swedish piglets with congenital tremor sampled in 2004 (n = 11) and 2011/2012 (n = 3) using the described APPVspecific RT-qPCR methods. The total number of piglets with signs of CT in this study was 29. Results: Atypical porcine pestivirus-RNA was detected in 93% (27/29) of the piglets suffering from congenital tremor. All piglets with congenital tremor from 2004 (n = 11) and 2012 (n = 3) were PCR-positive with respect to APPV, whereas, all of the healthy controls (n = 11) were negative. The piglets with congenital tremor sampled 2017-2018 had an odds ratio of 91.8 (95% CI 3.9128 to 2153.7842, z = 2.807, P = 0.0050) to test positive for APPV by qRT-PCR compared to the healthy piglets (Fishers exact test p < 0.0001). These findings make it interesting to continue investigating APPV in the Swedish pig-population. Conclusion: This is the first description of atypical porcine pestivirus in piglets suffering from congenital tremor type A-II in Sweden and the Nordic countries. The virus has been present in the Swedish pig population since at least 2004.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

2020

View full text Add to dashboard Cite

show abstract

“…Porcine circovirus‐like virus P1 has been detected in piglet herds in China and may cause clinical diseases, including PMWS and CT (Wen, He, Xiao et al., ; Wen, Mao, Jiao et al., ). In this study, 140 of 500 (28.0%) serum samples from 25 PMWS‐affected farms in seven cities of Hebei Province tested positive for P1 with PCR.…”

Section: Resultsmentioning

confidence: 99%

“…The P1 virus can infect several animals, including pigs, cattle, goats and rabbits, and is considered the aetiological agent of PMWS and congenital tremor (CT) (Wen, He, Xiao et al., ; Wen, He, Yu et al., ; Wen, Mao, Fan et al., ; Wen, Mao, Jiao et al. ).…”

Section: Introductionmentioning

confidence: 99%

Detection of porcine circovirus-like virus P1 in Hebei, China

Mao

Jiao

et al. 2018

Transbound Emerg Dis

Self Cite

View full text Add to dashboard Cite

Porcine circovirus-like virus P1 is a novel unclassified circovirus that was first detected in China and may be associated with post-weaning multisystemic wasting syndrome (PMWS) and congenital tremor. In this study, we detected P1 infection in pigs in Hebei Province, China, in 2017. One hundred and forty of 500 (28.0%) serum samples from 25 pig farms with different PMWS status in seven cities were P1 positive on PCR. Twelve P1 strains were sequenced, and the complete genomes of 11 P1 strains were 648 nucleotides (nt) in length, whereas that of strain ZJK02 was 647 nt, with a G deletion at position of 183 in its genome. The complete genomic and capsid protein sequences of the 12 P1 strains analysed in this study shared 98.8%-100.0% and 86.5%-100.0% identity, respectively. A phylogenetic analysis based on the complete genomic and capsid sequences of 26 P1 strains showed that the 12 P1 sequences from Hebei Province clustered on two small branches. Further studies of the evolution and pathogenesis of P1 are required.

show abstract

“…Since 2016, the etiology of CT A-II has been attributed to the newly described APPV, which has already been demonstrated by experimental infections [2,10]. Other viruses also were detected coinfection with APPV, such as porcine circovirus type 2 [11], astrovirus [12], porcine circovirus type 3 [13], linda virus [14], porcine circovirus-like virus P1 [15], and Teschovirus A [16], however, the role played by these three viruses as the primary cause of CT A-II is debatable.…”

Section: Congenital Tremor Syndromementioning

confidence: 93%

Pestivirus K (Atypical Porcine Pestivirus): Update on the Virus, Viral Infection, and the Association with Congenital Tremor in Newborn Piglets

Agnol

Alfieri

2020

Viruses

View full text Add to dashboard Cite

The atypical porcine pestivirus (APPV) belongs to the species Pestivirus K of the genus Pestivirus and the family Flaviviridae, and it has been associated with congenital tremor (CT) type A-II in newborn piglets. Although APPV was discovered in 2015, evidence shows that APPV has circulated in pig herds for many years, at least since 1986. Due to the frequently reported outbreaks of CT on different continents, the importance of this virus for global pig production is notable. Since 2015, several studies have been conducted to clarify the association between APPV and CT. However, some findings regarding APPV infection and the measures taken to control and prevent the spread of this virus need to be contextualized to understand the infection better. This review attempts to highlight advances in the understanding of APPV associated with type A-II CT, such as etiology, epidemiology, diagnosis, and control and prevention measures, and also describes the pathophysiology of the infection and its consequences for pig production. Further research still needs to be conducted to elucidate the host’s immune response to APPV infection, the control and prevention of this infection, and the possible development of vaccines.

show abstract

Evidence of porcine circovirus-like virus P1 in piglets with an unusual congenital tremor

Cited by 15 publications

References 11 publications

Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

Detection of porcine circovirus-like virus P1 in Hebei, China

Pestivirus K (Atypical Porcine Pestivirus): Update on the Virus, Viral Infection, and the Association with Congenital Tremor in Newborn Piglets

Contact Info

Product

Resources

About