Evidence of reduced vertical transmission of Neospora caninum associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition

Pereyra, Rodrigo; Mansilla, Florencia Celeste; Petersen, Marcos Iván; Suárez, Víctor Humberto; Capozzo, Alejandra Victoria

doi:10.1016/j.vetimm.2019.01.001

Cited by 8 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A balanced Th1/Th2 response has been considered beneficial in the course of neosporosis in pregnant mouse models by conferring protection against the parasite and avoiding fetal rejection by limiting deleterious excessive inflammation [25]. Moreover, an antibody profile with higher IgG1 than IgG2 antibodies combined with IFN-γ was previously associated with the reduced vertical transmission of N. caninum in cattle [60], which further highlights the potential of considering the Carbigen TM adjuvant in future studies of immunization against neosporosis in cattle. Although the murine model has been rightly considered a valuable experimental tool in preclinical studies in neosporosis [61], species-specific immune mechanisms should be accounted when evaluating immunization approaches to this parasitic disease.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect against Neosporosis Induced by Intranasal Immunization with Neospora caninum Membrane Antigens Plus Carbomer-Based Adjuvant

et al. 2022

View full text Add to dashboard Cite

Neospora caninum is an obligate intracellular protozoan responsible for abortion and stillbirths in cattle. We previously developed a mucosal vaccination approach using N. caninum membrane proteins and CpG adjuvant that conferred long-term protection against neosporosis in mice. Here, we have extended this approach by alternatively using the carbomer-based adjuvant Carbigen™ in the immunizing preparation. Immunized mice presented higher proportions and numbers of memory CD4+ and CD8+ T cells. Stimulation of spleen, lungs and liver leukocytes with parasite antigens induced a marked production of IFN-γ and IL-17A and, less markedly, IL-4. This balanced response was also evident in that both parasite-specific IgG1 and IgG2c were raised by immunization, together with specific intestinal IgA. Upon intraperitoneal infection with N. caninum, immunized mice presented lower parasitic burdens than sham-immunized controls. In the infected immunized mice, memory CD4+ T cells predominantly expressed T-bet and RORγt, and CD8+ T cells expressing T-bet were found increased. While spleen, lungs and liver leukocytes of both immunized and sham-immunized infected animals produced high amounts of IFN-γ, only the cells from immunized mice responded with high IL-17A production. Since in cattle both IFN-γ and IL-17A have been associated with protective mechanisms against N. caninum infection, the elicited cytokine profile obtained using CarbigenTM as adjuvant indicates that it could be worth exploring for bovine neosporosis vaccination.

show abstract

Section: Discussionmentioning

confidence: 99%

Protective Effect against Neosporosis Induced by Intranasal Immunization with Neospora caninum Membrane Antigens Plus Carbomer-Based Adjuvant

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Classically, N. caninum is associated with reproductive disorders in cattle, with mid-to late-term abortions as its main clinical feature (Thilsted and Dubey, 1989). An increasing number of research groups have published promising results on in vivo and ex vivo bovine immune responses against N. caninum during gestation (Andrianarivo et al, 2001;Rosbottom et al, 2008;Rojo-Montejo et al, 2009;Bartley et al, 2013;Hecker et al, 2015;Pereyra et al, 2019). Also, some groups adopt the gestational mouse model in order to draw their experimental conclusions on this topic (Long and Baszler, 2000;Arranz-Solís et al, 2015;Aguado-Martínez et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Interplay Between Reactive Oxygen Species and the Inflammasome Are Crucial for Restriction of Neospora caninum Replication

Mota

Lima-Junior

França

et al. 2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Neospora caninum poses as a considerable threat to animal health and generates significant economic impact in livestock production worldwide. Here, we have investigated the mechanism that underlies the participation of the inflammasome complex and Reactive Oxygen Species (ROS) in the regulation of immune responses during N. caninum infection. For that purpose, we used in vitro (bone marrow derived macrophages) and in vivo mouse models of infection. Our results show that NLRP3 and NLRC4 receptors, alongside with ASC and Caspase-1, are required for proper activation of the inflammasome during N. caninum infection. As expected, the engagement of these pathways is crucial for IL-1α, IL-1β, and IL-18 production, as well as the induction of pyroptosis. Our results also show that N. caninum induces ROS production dependent of the inflammasome assembly, which in its turn also depends on MyD88/NF-κB-induced ROS to maintain its activation and, ultimately, lead to restriction of parasite replication.

show abstract

“…High levels of IgG1 were also detected in the serum of mice in the NEV-and Nc14-3-3-immunized groups compared to those in the control groups, and the level of IgG1 antibodies was signi cantly higher than that of IgG2a, indicating that NEVs and Nc14-3-3 induced a mixed Th1/Th2 immune response. Type 1 immune responses are known to play an important role in protection against intracellular pathogens, a Th1-type immune response associated with high levels of IFN-γ and IL-12 [33].…”

Section: Discussionmentioning

confidence: 99%

Protective Immunity Against Neospora Caninum Infection Induced by 14-3-3 Protein in Mice

Zhang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundNeospora caninum causes infections in a wide range of intermediate hosts and remains a threatening disease worldwide because of the lack of effective drugs and vaccines. Our previous studies demonstrated that N. caninum 14-3-3 protein (Nc14-3-3), which is included in N. caninum extracellular vesicles (NEVs), can induce effective immune responses and stimulate cytokine expression in mouse peritoneal macrophages. However, whether Nc14-3-3 has a protective effect and its mechanisms are poorly understood.MethodsHere, we evaluated immune responses and protective effects of Nc14-3-3 against 2×107 Nc-1 tachyzoites. Antibody (IgG, IgGl and IgG2a) levels and Th1-type (IFN-γ and IL-12) and Th2-type (IL-4 and IL-10) cytokines in mouse serum; survival rates; survival time; and parasite burdens were detected.ResultsIn the present study, the immunostimulatory effect of Nc14-3-3 was confirmed, as it triggered Th1-type cytokine (IFN-γ and IL-12) production in mouse serum two weeks after the final immunization. Moreover, the immunization of C57BL/6 mice with Nc14-3-3 induced high IgG antibody levels and significant increases in CD8+ T lymphocytes in the spleens of mice, indicating that a significant cellular immune response was induced. Mouse survival rates and survival times were significantly prolonged after immunization survival rates were 40% for Nc14-3-3 immunization and 60% for NEV immunization, while mice that received GST, PBS, or blank control all died at 13, 9, and 8 days after intraperitoneal N. caninum challenge. In addition, qPCR analysis indicated that there was a lower parasite burden and milder pathological changes in the mice immunized with Nc14-3-3.ConclusionsOur data demonstrate the vaccination of mice with Nc14-3-3 elicits both cellular and humoural immune responses and provides partial protection against acute neosporosis. Thus, Nc14-3-3 could be an effective antigen candidate for vaccine development for neosporosis.

show abstract

Evidence of reduced vertical transmission of Neospora caninum associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition

Cited by 8 publications

References 27 publications

Protective Effect against Neosporosis Induced by Intranasal Immunization with Neospora caninum Membrane Antigens Plus Carbomer-Based Adjuvant

Protective Effect against Neosporosis Induced by Intranasal Immunization with Neospora caninum Membrane Antigens Plus Carbomer-Based Adjuvant

Interplay Between Reactive Oxygen Species and the Inflammasome Are Crucial for Restriction of Neospora caninum Replication

Protective Immunity Against Neospora Caninum Infection Induced by 14-3-3 Protein in Mice

Contact Info

Product

Resources

About