Evidence of tricellulin expression by immune cells, particularly microglia

Mariano, Cibelle; Silva, Sandra Leitão; Pereira, Pedro; Fernandes, Adelaide; Brites, Dora; Brito, Maria Alexandra

doi:10.1016/j.bbrc.2011.05.093

Cited by 17 publications

(8 citation statements)

References 29 publications

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“…Since the C-terminus cytoplasmic sequence of tricellulin is highly similar to that of occludin, tricellulin might also bind to various kinases. Tricellulin is also thought to be present along the lateral membrane at tricellular tight junctions (Raleigh et al 2010; Mariano et al 2011). In the present study, at 4 h after Ca 2+ repletion, tricellulin induced in the Triton-X-100-insoluble fraction and phosphorylated on threonine residues was localized not only at tricellular contacts but also in cytoplasm and at bicellular borders as shown by immunofluorescence microscopy and immuno-TEM analysis (Fig.…”

Section: Discussionmentioning

confidence: 99%

Behavior of tricellulin during destruction and formation of tight junctions under various extracellular calcium conditions

et al. 2012

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Tricellulin is an important component of tricellular tight junctions (TJs) and is involved in the formation of tricellular contacts. However, little is known about its regulation during the assembly and disassembly of tricellular TJs. By using the well-differentiated pancreatic cancer cell line HPAC, which highly expresses tricellulin at tricellular contacts, we have investigated changes in the localization, expression and phosphorylation of tricellulin and in its TJ functions as a barrier and fence during the destruction and formation of TJs induced by changes in the extracellular calcium concentration. During both extracellular Ca2+ depletion caused by EGTA treatment and Ca2+ repletion after Ca2+ starvation, the expression of tricellulin increased in whole lysates and in Triton-X-100-insoluble fractions without any change in its mRNA. The increases in immunoreactivity revealed by Western blotting were prevented by alkaline phosphatase treatment. Immunoprecipitation assays showed that tricellulin was phosphorylated on threonine residues when it increased after Ca2+ depletion and repletion. In the early stage after Ca2+ repletion, tricellulin was expressed not only at tricellular contacts but also in the cytoplasm and at bicellular borders. In confocal laser microscopy, tricellulin was observed at the apical-most regions and basolateral membranes of tricellular contacts after Ca2+ repletion. Knockdown of tricellulin delayed the recovery of the barrier and fence functions after Ca2+ repletion. Thus, the dynamic behavior of tricellulin during the destruction and formation of TJs under various extracellular calcium conditions seems to be closely associated with the barrier and fence functions of TJs.Electronic supplementary materialThe online version of this article (doi:10.1007/s00441-012-1512-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Behavior of tricellulin during destruction and formation of tight junctions under various extracellular calcium conditions

et al. 2012

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“…Tricellulin is widely expressed, including in the inner ear, skin, stomach, and nasal epithelial cells (112), but its expression in the lung has not been well studied. Tricellulin is also expressed by nonepithelial myeloid cells (e.g., brain microglia [113]), but whether myeloid cells express this molecule in the lung requires further study. The expression and function of the remaining MARVEL family member MARVELD3 in the lung has not been previously reported to our knowledge.…”

Section: Other Moleculesmentioning

confidence: 99%

Breaking Barriers. New Insights into Airway Epithelial Barrier Function in Health and Disease

Rezaee

Georas

2014

Am J Respir Cell Mol Biol

132

124

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Epithelial permeability is a hallmark of mucosal inflammation, but the molecular mechanisms involved remain poorly understood. A key component of the epithelial barrier is the apical junctional complex that forms between neighboring cells. Apical junctional complexes are made of tight junctions and adherens junctions and link to the cellular cytoskeleton via numerous adaptor proteins. Although the existence of tight and adherens junctions between epithelial cells has long been recognized, in recent years there have been significant advances in our understanding of the molecular regulation of junctional complex assembly and disassembly. Here we review the current thinking about the structure and function of the apical junctional complex in airway epithelial cells, emphasizing the translational aspects of relevance to cystic fibrosis and asthma. Most work to date has been conducted using cell culture models, but technical advancements in imaging techniques suggest that we are on the verge of important new breakthroughs in this area in physiological models of airway diseases.

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“…These observations suggest that tTJs can form between different cell types, namely epithelial cells and immune cells. Tricellulin is also expressed in other non-epithelial cells, including glial cells in the brain 30 and immune cells of the monocyte/macrophage lineage and microglia 33 . In mouse myelinating Schwann cells, tricellulin is concentrated at autotypic TJs of myelin 34 …”

Section: Expression Of Tricellulin and Angulins In Non-epithelial Cellsmentioning

confidence: 99%

Molecular organization of tricellular tight junctions

et al. 2014

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When the apicolateral border of epithelial cells is compared with a polygon, its sides correspond to the apical junctional complex, where cell adhesion molecules assemble from the plasma membranes of two adjacent cells. On the other hand, its vertices correspond to tricellular contacts, where the corners of three cells meet. Vertebrate tricellular contacts have specialized structures of tight junctions, termed tricellular tight junctions (tTJs). tTJs were identified by electron microscopic observations more than 40 years ago, but have been largely forgotten in epithelial cell biology since then. The identification of tricellulin and angulin family proteins as tTJ-associated membrane proteins has enabled us to study tTJs in terms of not only the paracellular barrier function but also unknown characteristics of epithelial cell corners via molecular biological approaches.

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Evidence of tricellulin expression by immune cells, particularly microglia

Cited by 17 publications

References 29 publications

Behavior of tricellulin during destruction and formation of tight junctions under various extracellular calcium conditions

Behavior of tricellulin during destruction and formation of tight junctions under various extracellular calcium conditions

Breaking Barriers. New Insights into Airway Epithelial Barrier Function in Health and Disease

Molecular organization of tricellular tight junctions

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