Evidence That Adhesive Sites on the Tips of Oviduct Cilia Membranes Are Required for Ovum Pickup in situ1

Norwood, J. T.; Anderson, Richard G. W.

doi:10.1095/biolreprod23.4.788

Cited by 33 publications

(11 citation statements)

References 12 publications

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“…The factor(s) involved in irreversible inhibition of OPR has not yet been identified but appears to decrease adhesion between the OCC and cilia [13,26]. In the absence of adhesion, even cilia that are beating normally are not able to pick up an OCC [26,[29][30][31]. In the current study, we did not observe any effect of KCN on adhesion of the OCC to the cilia.…”

Section: Discussionmentioning

confidence: 48%

Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

Talbot¹,

DiCarlantonio

Knöll

et al. 1998

Biology of Reproduction

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Previous studies have shown that solutions of mainstream and sidestream cigarette smoke decrease both ciliary beat frequency and oocyte cumulus complex pick-up rate of hamster oviducts in vitro. The purpose of this study was to identify the component(s) in smoke that produces these effects. Chemicals reported to be ciliotoxic in other systems were tested on hamster infundibula at various concentrations to determine whether a dose-response inhibition of ciliary beat frequency occurred. In addition, the concentration of each test component was measured in mainstream and sidestream smoke solutions (whole, particulate, and gas phase). All test components (acrolein, formaldehyde, phenol, acetaldehyde, and potassium cyanide [KCN]) inhibited ciliary beat frequency in a dose-dependent manner. Inhibition of ciliary beat frequency was at least partially reversible for all test compounds except acrolein. The concentrations of acrolein, formaldehyde, and phenol that were required to inhibit beat frequency were at least 3-50 times higher than their corresponding concentrations in smoke solutions. In contrast, cyanide was present in all smoke solutions at concentrations sufficient to inhibit ciliary beat frequency. Cilia on the outer surface of the infundibulum function in picking up the oocyte cumulus complex. Oocyte cumulus complex pick-up rate was measured in vitro at KCN concentrations shown to inhibit ciliary beat frequency. Pick-up rate was likewise inhibited by KCN and remained significantly depressed after washout of KCN. These data show that cyanide is a potent inhibitor of both ciliary beat frequency and oocyte cumulus complex pick-up rate and that its concentration in smoke solutions is sufficiently high to explain the previously reported inhibition of these oviductal processes.

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Section: Discussionmentioning

confidence: 48%

Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

Talbot¹,

DiCarlantonio

Knöll

et al. 1998

Biology of Reproduction

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“…OCC pick-up requires adhesion between the tips of the cilia and the cumulus matrix, and factors that increase or decrease adhesion inhibit the rate of pick-up (Lam et al, 2000;Mahi-Brown & Yanagimachi, 1983;Norwood & Anderson, 1980). Inhalation of either MS or SS smoke prior to and during pregnancy increased the number of blebs on the oviductal epithelium of hamsters indicating smoke targets these cells (Magers et al, 1995).…”

Section: Ovulation Through Fertilizationmentioning

confidence: 99%

The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells

Talbot

Lin

2011

Biol. Res.

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Numerous studies have repeatedly shown that women who smoke experience problems establishing and maintaining pregnancies, and recent work has further demonstrated that the in utero effects of smoke may not be manifested until months or even years after birth. The purpose of this review is to examine the recent literature dealing with the effects of cigarette smoke on the earliest stages of human prenatal development. Studies in this area have included the use of animal models, patients undergoing in vitro fertilization, and embryonic stem cell models. Events leading to fertilization, such as cumulus expansion, hyperactivation of sperm motility, and oocyte pick-up by the oviduct are all impaired by smoke exposure in animal models. Steps crucial to fertilization such as the acrosome reaction and sperm binding to the zona pellucida are likewise inhibited by cigarette smoke. Preimplantation embryos and stem cells that model embryos show a number of adverse responses to smoke exposure, including poor adhesion to extracellular matrices, diminished survival and proliferation, and increased apoptosis. The current literature demonstrates that the earliest stages of prenatal development are sensitive to smoke exposure and indicates that pregnant women should be advised not to smoke during this time.

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“…The wall of the oviduct contains smooth muscle that is particularly abundant in the isthmus. Pick-up of the OCC by the oviduct depends on adhesion of the cumulus matrix to the tips of the cilia and beating of the cilia toward the ostium (opening of the infundibulum) (Norwood and Anderson, 1980;Mahi-Brown and Yanagimachi, 1983;Talbot et al, 2000). The oocyte enters the oviduct rapidly after ovulation then slows its movement in the ampulla where fertilization occurs.…”

Section: Oviductsmentioning

confidence: 99%

In vitro assessment of reproductive toxicity of tobacco smoke and its constituents

Talbot

2008

Birth Defects Research Pt C

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Epidemiological studies have repeatedly shown that reproductive processes in pregnant women are adversely affected by exposure to cigarette smoke. The potential reproductive targets of smoke during pregnancy include the ovaries, oviducts, uterus, placenta, umbilical cord, and embryo/fetus. In vitro methods for studying the effects of smoke and its individual components have been developed and applied to each of these reproductive targets. In vitro assays have been useful in determining the biological processes that are affected in the reproductive organs and in identifying the cellular and molecular targets of smoke in each organ. In vitro methods have also been used to study the mechanism of action of smoke constituents, such as nicotine, on specific processes in reproductive organs and to screen smoke solutions to identify the molecules that affect reproduction. In general, data collected in vitro have confirmed, extended, and helped explain what has been learned from epidemiological studies. This review summarizes some of the in vitro assays that have been used to study cigarette smoke's effect on the nonpregnant and pregnant female reproductive tract and spotlights examples of their applications.

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Evidence That Adhesive Sites on the Tips of Oviduct Cilia Membranes Are Required for Ovum Pickup in situ1

Cited by 33 publications

References 12 publications

Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

Identification of Cigarette Smoke Components That Alter Functioning of Hamster (Mesocricetus auratus) Oviducts In Vitro1

The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells

In vitro assessment of reproductive toxicity of tobacco smoke and its constituents

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