Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes’ IgG

Fratrić, Natalija; Gvozdić, Dragan; Savić, Olivera; Buač, Marijana; Ilić, Vesna

doi:10.1016/j.vetimm.2012.09.009

Cited by 5 publications

(8 citation statements)

References 48 publications

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“…Cultures of bronchoalveolar lavage fl uids are considered to be the most reliable for the identifi cation of causative microbial agents but DeRosa et al [25] showed that calve's nasal swab cultures are representative of isolates present in the lungs and Taylor et al [26] showed that nasal sampling, when combined with clinical diagnosis, may provide greater diagnostic accuracy and improve treatments. In this study, as well as in our previous studies [8,11] conducted during 2012-2016 period on the same farm, a gram negative bacterium, P. multocida, was abundantly found in nasal swabs of all diseased calves while other pathogenic bacteria or fungi were not isolated. These results indicate the most important role of this bacterium for the development of bronchopneumonia in the analyzed farm.…”

Section: Discussionsupporting

confidence: 75%

“…Electrophoresis in 1% agarose gel buffered with 92 mM sodium-barbital buffer, pH 8.6 was used for analysis of serum proteins [8,11]. The relative content of serum protein fractions (γ, δ, and α globulins and albumin) was quantifi ed by densitometry using ImageMaster TotalLab TL 120 software (GE HealthCare LifeScience, NJ, USA).…”

Section: Protein Agarose Gel Electrophoresismentioning

confidence: 99%

“…Non-reducing SDS-PAGE of calves sera (5 μg proteins per well, 10-well comb) in 8%, 0.8 mm gels was carried out in Perfect Blue™ Doppler Gel system Twin S (Peqlab Ltd., United Kingdom) [8]. PageRulerTM Plus molecular size markers size (250, 130, 100, 70, 55, 35, 25, 15 and 10 kDa) (Thermo Scientifi c, Rockford, IL, USA) were used.…”

Section: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (Smentioning

confidence: 99%

“…Even nowadays the diagnosis of calf bronchopneumonia is based mostly on clinical observation. Isolation of causative microbiological agents and hematological, biochemical, and immunological analyses have been widely applied in epidemiological surveys and basic and applied research of the disease pathogenesis [2][3][4][5][6][7][8][9][10][11]. Most of these methods are available for veterinary practice; however, they are extremely rarely, if at all, used for the diagnosis of calf bronchopneumonia.…”

Section: Introductionmentioning

confidence: 99%

“…Although acute phase proteins (haptoglobin, serum amyloid A, alpha(1)-acid glycoprotein, fi brinogen) have been considered to be reliable biomarkers of the diseases [5][6][7]12,13] a recent meta-analysis has cast doubt on it [14]. Further acute phase proteins, peripheral blood leukocyte count, hemoglobin concentration, blood CO 2 and O 2 , circulating immune complexes, serum protein fractions, alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), and low-density lipoprotein (LDL) cholesterol have also been considered as reliable disease markers [8,[11][12][13][15][16][17][18] but the results are not always consistent.…”

Section: Introductionmentioning

confidence: 99%

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Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers

et al. 2017

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Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDS-PAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and α globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total γ globulins (IgG), due to an increased concentration of anionic γ globulins (predominately IgG1), was detected. The increased concentration of anionic γ globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.

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Section: Discussionsupporting

confidence: 75%

Section: Protein Agarose Gel Electrophoresismentioning

confidence: 99%

Section: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers

et al. 2017

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Immunoglobulin G glycosylation in aging and diseases

Gudelj

Lauc

Pezer

2018

Cellular Immunology

343

345

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The Immunoglobulin G (IgG) glycome is well known for its heterogeneity and shows a significant degree of variation within populations. IgG glycome composition is influenced both by genes and by environment, making it an excellent biomarker of a person's general health state, i.e. biological age. IgG glycosylation appears to be highly regulated, both during homeostasis and in cases of its disturbance. Changes in IgG glycosylation patterns have been observed in aging and in various diseases. Differential IgG glycosylation is known to modulate IgG effector functions and is involved in disease development and progression, representing both a predisposition and a functional mechanism involved in disease pathology. This makes IgG glycosylation analysis a promising add-on to improve existing disease biomarkers.

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Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation

Buač

Mojsilović

Mišić

et al. 2016

Research in Veterinary Science

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Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes’ IgG

Cited by 5 publications

References 48 publications

Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers

Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers

Immunoglobulin G glycosylation in aging and diseases

Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation

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