Evidence that endogenous gamma interferon is produced early in Listeria monocytogenes infection

Abstract

It has been presumed that gamma interferon (IFN-gamma), which plays an essential role in antilisterial resistance, is produced late in Listeria monocytogenes infection. In the present study, however, IFN-gamma was detected in the bloodstreams and spleens of mice from days 1 to 4 of L. monocytogenes infection by both a double-sandwich enzyme-linked immunosorbent assay and an immunohistochemical technique, suggesting that endogenous IFN-gamma is produced early but not late in L. monocytogenes infection.

Endogenous gamma interferon produced in central nervous system by systemic infection infection with Theiler's virus in mice

“…There are only a few reports on the kinetics of endogenous IFN-7 production during infections. In listerial infection, endogenous IFN-y production coincided with bacterial growth (Nakane et al, 1990). Our present data also indicate that endogenous IFN-y production coincides with the viral replication (Fig.…”

“…IFN-y assay of the tissue homogenate was carried out by a double-sandwich enzyme-linked immunosorbent assay (ELISA) as previously reported (Nakane et al, 1990). Rat anti-mouse IFN-y mAb (R4-6A2) purified by DEAE Affi-Gel Blue column chromatography (Nakane et al, 1989) was used as a capture antibody.…”

Investigation of the role of macrophages and endogenous interferon-γ in natural resistance of mice against Legionella pneumophila infection

“…It is known that IFN-7, by activating macrophages, plays an important role in host defense against many bacteria [reviewed by Murray, 19]. IFN-7 was produced in the early phase of listerial infection in mice [20]. Blanchard et al reported that in vitro production of IFN-7 from mouse splenocytes could be detected as early as 6 h after stimulation with the L. pneumophila antigen, continued to increase for up to 24 h after stimulation and thereafter almost completely ceased [13].…”

Theiler's virus is eliminated by a gamma-interferon-independent mechanism in the brain

“…IFN-3, assay in the tissue homogenate was carried out by a double-sandwich enzyme-linked immunosor-bent assay (ELISA) as previously reported (Nakane et al, 1990). Rat anti-mouse IFN-~/ mAb (R46A2) (Spintalny and Havell, 1984) purified by DEAE Affi-Gel Blue Column chromatography was used as a capture antibody.…”

“…Rat anti-mouse IFN-~/ mAb (R46A2) (Spintalny and Havell, 1984) purified by DEAE Affi-Gel Blue Column chromatography was used as a capture antibody. Rabbit anti-mouse IFN-y serum was obtained from a rabbit hyperimmunized with purified recombinant mouse IFN-3, as that described previously (Nakane et al, 1990). All ELISAs were run with recombinant mouse IFN-7 that was produced and purified by Genentech, Inc. (San Francisco, CA).…”