Evidence to Support Karyotypic Variation of the Mosquito,Anopheles peditaeniatusin Thailand

Abstract

Eight isoline colonies of Anopheles peditaeniatus Leicester (Diptera: Culicidae) were established from wild-caught females collected from buffalo-baited traps at 8 localities in Thailand. They showed 2 types of X (X2, X3) and 4 types of Y (Y2, Y3, Y4, Y5) chromosomes based on the number and amount of major block(s) of heterochromatin present in the heterochromatic arm, and were tentatively designated as Forms B (X2, X3, Y2), C (X3, Y3), D (X3, Y4) and E (X2, X3, Y5). Form B was found in Nan, Ratchaburi, and Chumphon provinces; Form C was obtained in Chon Buri province; Form D was recovered in Kamphaeng Phet province; and Form E was acquired in Chiang Mai, Udon Thani, and Ubon Ratchathani provinces. Crossing studies among the 8 isoline colonies, which were representative of 4 karyotypic forms of An. peditaeniatus, revealed genetic compatibility in providing viable progenies and synaptic salivary gland polytene chromosomes through F2-generations, thus suggesting the conspecific nature of these karyotypic forms. These results were supported by the very low intraspecific sequence variations (0.0 – 1.1%) of the nucleotide sequences in ribosomal DNA (ITS2) and mitochondrial DNA (COI and COII) of the 4 forms.

Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia

“…Obviously, the above information indicated the possibility of a cytological mechanism for the karyotypic evolution of the Oriental Anopheles by gradually adding extra heterochromatin onto the arms of sex chromosomes, which is in keeping with hypothesis of Baimai [22]. Additionally, such chromosome distinction is very useful for the cytotaxonomic study of closely related species, especially sibling species and/or subspecies of Anopheles, as exemplified in other groups of Oriental anophelines [8,11,[23][24][25][26][27][28][29][30][31][32].…”

“…Cross-mating experiments using isoline colonies of anopheline mosquitoes, which relate to results of cytology and molecular analysis to determine post-mating barriers, have proven to be an efficient technique for identifying sibling species and/or subspecies within Anopheles [8,11,[23][24][25][26][27][28][29][30][31][32]. Regarding this matter, cross-matings among the four allopatric karyotypic forms of A. crawfordi were performed intensively.…”

Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand

“…barbirostris species A1 (Saeung et al 2007), Anopheles campestrislike taxon ) and An. peditaeniatus (Choochote 2011). Our findings in this study showed no post-mating reproductive isolation between An.…”

In Vitro Propagation of Kaempferia Galanga (Zingiberaceae) and Comparison of Larvicidal Activity and Phytochemical Identities of Rhizomes of Tissue Cultured and Naturally Grown Plants

“…The effect is dose dependent, long lasting and statistically significant [15]. Hexane extract of the rhizome has been used to test the mosquito repellent activity [16]. The results of this preliminary study could be used in developing a commercial herbal powder to use as a mosquito repellent for mosquito species namely-A.aegypti, A. albopictus, Culex sp.…”

“…Thus, comparison of phytochemicals present in rhizomes of natural plants and tissue cultured plants are necessary if the tissue cultured plants are using as alternative to natural plants. 16 hours photoperiod. Results were analyzed using Minitab statistical package.…”