Evolution of ketosynthase domains of polyketide synthase genes in the Cladonia chlorophaea species complex (Cladoniaceae)

Timsina, Brinda A.; Hausner, Georg; Piercey-Normore,

doi:10.1016/j.funbio.2014.08.001

Cited by 15 publications

(3 citation statements)

References 60 publications

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“…To detect possible polyketide synthase genes encoded within the C. uncialis genome, four pairs of degenerate PCR primers were employed: LC3/LC5c | PKS1F/PKS2R | PKSDA13F/PKSDA13R | PKS1F1/JSAT2R (Table S1 in the Supporting Information). The original use of the first three primer pairs is described elsewhere and is designed to target a segment of the ketosynthase domain. The fourth primer pair targets a longer stretch of DNA covering both the KS and AT domains and was designed based on conserved DNA regions found in lichen PKS genes deposited in GenBank (this study).…”

Section: Methodsmentioning

confidence: 99%

Identification of 6-Hydroxymellein Synthase and Accessory Genes in the Lichen Cladonia uncialis

et al. 2016

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A transcribed polyketide synthase (PKS) gene has been identified in the lichen Cladonia uncialis. The complete nucleotide sequence of this PKS was determined from the amplified cDNA, and an assignment of individual domains was accomplished by homology searching using AntiSMASH. A scan of the complete genome sequence of C. uncialis revealed the accessory genes associated with this PKS gene. A homology search has identified that several genes in this cluster are similar to genes responsible for the biosynthesis of terrein in Aspergillus terreus. This permitted assignment of putative function to each of the genes in this new C. uncialis cluster. It is proposed that this gene cluster is responsible for the biosynthesis of a halogenated iscoumarin. This is the first report linking a gene cluster to a halogenated metabolite in lichen.

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Section: Methodsmentioning

confidence: 99%

Identification of 6-Hydroxymellein Synthase and Accessory Genes in the Lichen Cladonia uncialis

et al. 2016

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“…Compared to the previous annotation [16], the newly annotated genome contained 10,818 protein-coding genes, among which 39 genes encode iterative type I PKS. The PKS genes included PKS1, PKS2, PKS3, PKS5, PKS7, PKS10, PKS11, PKS13, PKS15, and PKS16, which were originally described in an earlier study on the Cladonia chlorophaea species complex [52]. Among these previously annotated PKSs, PKS1 was predicted to be involved in depside-and depsidoneclass metabolites derived from 3-methylorsellinic acid [12,53].…”

Section: Gene Expression Profiling Of Pks Genes In Cladonia Metacoral...mentioning

confidence: 99%

Identification of a biosynthetic gene cluster for a red pigment cristazarin produced by a lichen-forming fungus Cladonia metacorallifera

et al. 2023

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Lichens are known to produce many novel bioactive metabolites. To date, approximately 1,000 secondary metabolites have been discovered, which are predominantly produced by the lichen mycobionts. However, despite the extensive studies on production of lichen secondary metabolites, little is known about the responsible biosynthetic gene clusters (BGCs). Here, we identified a putative BGC that is implicated in production of a red pigment, cristazarin (a naphthazarin derivative), in Cladonia metacorallifera. Previously, cristazarin was shown to be specifically induced in growth media containing fructose as a sole carbon source. Thus, we performed transcriptome analysis of C. metacorallifera growing on different carbon sources including fructose to identify the BGC for cristazarin. Among 39 polyketide synthase (PKS) genes found in the genome of C. metacorallifera, a non-reducing PKS (coined crz7) was highly expressed in growth media containing either fructose or glucose. The borders of a cristazarin gene cluster were delimited by co-expression patterns of neighboring genes of the crz7. BGCs highly conserved to the cristazarin BGC were also found in C. borealis and C. macilenta, indicating that these related species also have metabolic potentials to produce cristazarin. Phylogenetic analysis revealed that the Crz7 is sister to fungal PKSs that biosynthesize an acetylated tetrahydoxynaphthalene as a precursor of melanin pigment. Based on the phylogenetic placement of the Crz7 and putative functions of its neighboring genes, we proposed a plausible biosynthetic route for cristazarin. In this study, we identified a lichen-specific BGC that is likely involved in the biosynthesis of a naphthazarin derivative, cristazarin, and confirmed that transcriptome profiling under inducing and non-inducing conditions is an effective strategy for linking metabolites of interest to biosynthetic genes.

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“…2016). In spite of the extended studies dealing with this genus, including also recent molecular phylogenetic studies (DePriest 1994, 1995; Timsina et al . 2014; Stenroos et al .…”

Section: Introductionmentioning

confidence: 99%

Analysis of Lichen Secondary Metabolites and Morphometrics in the Cladonia chlorophaea Species Group (Cladoniaceae, Lichenized Ascomycota) in Hungary

Farkas

Lőkös

Veres

2023

Cryptogamie, Mycologie

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Evolution of ketosynthase domains of polyketide synthase genes in the Cladonia chlorophaea species complex (Cladoniaceae)

Cited by 15 publications

References 60 publications

Identification of 6-Hydroxymellein Synthase and Accessory Genes in the Lichen Cladonia uncialis

Identification of 6-Hydroxymellein Synthase and Accessory Genes in the Lichen Cladonia uncialis

Identification of a biosynthetic gene cluster for a red pigment cristazarin produced by a lichen-forming fungus Cladonia metacorallifera

Analysis of Lichen Secondary Metabolites and Morphometrics in the Cladonia chlorophaea Species Group (Cladoniaceae, Lichenized Ascomycota) in Hungary

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