Evolution of P2A and P5A ATPases: ancient gene duplications and the red algal connection to green plants revisited

Palmgren, Michael G.; Sørensen, Danny Mollerup; Hallström, Björn M.; Säll, Torbjörn; Broberg, Karin

doi:10.1111/ppl.13008

Cited by 11 publications

(13 citation statements)

References 66 publications

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“…As is presented in Figure 2 , the homologs of ECA3s and CA8s have existed in all tested clades of the land plant lineage with high similarity (Bryophyta, Lycophyta, Ferns, Gymnosperm, and angiosperm), as well as the algae (Glaucophyta, Rhodophyta, Chlorophyta, and Streptophytina). This is consisting with the previous study that the P2A ATPases and P2B ATPases are common in both prokaryotes and eukaryotes ( Palmgren et al, 2020 ; Figure 2 ).…”

Section: Resultssupporting

confidence: 92%

Evolutionary and Regulatory Pattern Analysis of Soybean Ca2+ ATPases for Abiotic Stress Tolerance

Wang

Zhang

et al. 2022

Front. Plant Sci.

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P2-type Ca2+ ATPases are responsible for cellular Ca2+ transport, which plays an important role in plant development and tolerance to biotic and abiotic stresses. However, the role of P2-type Ca2+ ATPases in stress response and stomatal regulation is still elusive in soybean. In this study, a total of 12 P2-type Ca2+ ATPases genes (GmACAs and GmECAs) were identified from the genome of Glycine max. We analyzed the evolutionary relationship, conserved motif, functional domain, gene structure and location, and promoter elements of the family. Chlorophyll fluorescence imaging analysis showed that vegetable soybean leaves are damaged to different extents under salt, drought, cold, and shade stresses. Real-time quantitative PCR (RT-qPCR) analysis demonstrated that most of the GmACAs and GmECAs are up-regulated after drought, cold, and NaCl treatment, but are down-regulated after shading stress. Microscopic observation showed that different stresses caused significant stomatal closure. Spatial location and temporal expression analysis suggested that GmACA8, GmACA9, GmACA10, GmACA12, GmACA13, and GmACA11 might promote stomatal closure under drought, cold, and salt stress. GmECA1 might regulate stomatal closure in shading stress. GmACA1 and GmECA3 might have a negative function on cold stress. The results laid an important foundation for further study on the function of P2-type Ca2+ ATPase genes GmACAs and GmECAs for breeding abiotic stress-tolerant vegetable soybean.

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Section: Resultssupporting

confidence: 92%

Evolutionary and Regulatory Pattern Analysis of Soybean Ca2+ ATPases for Abiotic Stress Tolerance

Wang

Zhang

et al. 2022

Front. Plant Sci.

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“…Not considered in detail here is active Ca 2+ efflux at the plasmalemma that maintains the very low free Ca 2+ concentration in the cytosol (100–200 μmol m −3 ) used in preventing damage to proteins and in signalling (Roberts et al ., 1994; Thompson et al ., 2007; Wheeler et al ., 2019). Ca 2+ -H + antiporters and Ca 2+ P-ATPases that are involved in Ca 2+ efflux from the cytosol are known, on genomic evidence, to be very widespread among algae (Emery et al ., 2012; Palmgren et al ., 2020).…”

Section: Possible Original Functions Of Primary Active H+ Na+ and CLmentioning

confidence: 99%

“…Almost all eukaryotic photolithotrophs have a photosynthetic apparatus most of whose core genes were derived from endosymbiosis of a freshwater β-cyanobacterium with a non-photosynthetic unicellular eukaryote to comprise the Archaeplastida (Burki et al ., 2020; Palmgren et al ., 2020). ‘Green’ Archaeplastida with chlorophyll b produced, by endosymbiosis in an Excavate cell, the secondarily photosynthetic Euglenophyta and, by endosymbiosis in a cell of the Rhizaria in the TSAR (Telonemia, Stramenopila, Alveolata, Rhizaria) supergroup, the secondarily photosynthetic Chlorarachniophyta.…”

Section: Organisms With Chlorophyllsmentioning

confidence: 99%

“…It is these ions that are the focus of the subsequent discussion in this paper. Other important ions subject to primary active transport are Ca 2+ and Mg 2+ , transported out of cells by P-type ATPases, that also pump, in various organisms, Na + out:K + in, K + in, Na + out, H + out and H + out:K + in (Kuhlbrandt, 2004; Bublitz et al ., 2011; Chan et al ., 2011, 2012; Palmgren & Nissen, 2011; Søndergaard & Pedersen, 2015; Palmgren et al ., 2020). As well as variants pumping H + , Na + , K + , Mg 2+ and Ca 2+ (topological type II: Thever & Saier, 2009, in prokaryotes and eukaryotes), there are also P-type ATPases (topological type I, Thever & Saier, 2009, not considered further here) that pump out metal cations such as Cu(I), Ag(I), Zn(II), Cd(II) and Pb(II) (Kuhlbrandt, 2004; Thever & Saier, 2009; Bublitz et al ., 2011; Palmgren & Nissen, 2011; Søndergaard & Pedersen, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Energizing the plasmalemma of marine photosynthetic organisms: the role of primary active transport

Raven

Beardall

2020

J. Mar. Biol. Ass.

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Generation of ion electrochemical potential differences by primary active transport can involve energy inputs from light, from exergonic redox reactions and from exergonic ATP hydrolysis. These electrochemical potential differences are important for homoeostasis, for signalling, and for energizing nutrient influx. The three main ions involved are H+, Na+ (efflux) and Cl− (influx). In prokaryotes, fluxes of all three of these ions are energized by ion-pumping rhodopsins, with one archaeal rhodopsin pumping H+into the cells; among eukaryotes there is also an H+ influx rhodopsin in Acetabularia and (probably) H+ efflux in diatoms. Bacteriochlorophyll-based photoreactions export H+ from the cytosol in some anoxygenic photosynthetic bacteria, but chlorophyll-based photoreactions in marine cyanobacteria do not lead to export of H+. Exergonic redox reactions export H+ and Na+ in photosynthetic bacteria, and possibly H+ in eukaryotic algae. P-type H+- and/or Na+-ATPases occur in almost all of the photosynthetic marine organisms examined. P-type H+-efflux ATPases occur in charophycean marine algae and flowering plants whereas P-type Na+-ATPases predominate in other marine green algae and non-green algae, possibly with H+-ATPases in some cases. An F-type Cl−-ATPase is known to occur in Acetabularia. Some assignments, on the basis of genomic evidence, of P-type ATPases to H+ or Na+ as the pumped ion are inconclusive.

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“…Phylogenetic Analysis. Detailed phylogenetic analysis is described in Palmgren et al 23,25 Sequences with significant similarity (expect value of <e −50 ) to the well-characterized Homo sapiens AS3MT were identified in the NCBI protein database using the Basic Local Alignment Search Tool (BLAST) program (http://blast.ncbi. nlm.nih.gov/).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Predicted AS3MT Proteins Methylate Arsenic and Support Two Major Phylogenetic AS3MT Groups

Pedersen

Loma

Levi

et al. 2020

Chem. Res. Toxicol.

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Inorganic arsenic is one of the most toxic and carcinogenic substances in the environment, but many organisms, including humans, methylate inorganic arsenic to mono-, di-, and trimethylated arsenic metabolites, which the organism can excrete. In humans and other eukaryotic organisms, the arsenite methyltransferase (AS3MT) protein methylates arsenite. AS3MT sequences from eukaryotic organisms group phylogenetically with predicted eubacterial AS3MT sequences, which has led to the suggestion that AS3MT was acquired from eubacteria by multiple events of horizontal gene transfer. In this study, we evaluated whether 55 (out of which 47 were predicted based on protein sequence similarity) sequences encoding putative AS3MT orthologues in 47 species from different kingdoms can indeed methylate arsenic. Fifty-three of the proteins showed arsenic methylating capacity. For example, the predicted AS3MT of the human gut bacterium Faecalibacterium prausnitzii methylated arsenic efficiently. We performed a kinetic analysis of 14 AS3MT proteins representing two phylogenetically distinct clades (Group 1 and 2) that each contain both eubacterial and eukaryotic sequences. We found that animal and bacterial AS3MTs in Group 1 rarely produce trimethylated arsenic, whereas Hydra vulgaris and the bacterium Rhodopseudomonas palustris in Group 2 produce trimethylated arsenic metabolites. These findings suggest that animals during evolution have acquired different arsenic methylating phenotypes from different bacteria. Further, it shows that humans carry two bacterial systems for arsenic methylation: one bacterium-derived AS3MT from Group 1 incorporated in the human genome and one from Group 2 in F. prausnitzii present in the gut microbiome.

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Evolution of P2A and P5A ATPases: ancient gene duplications and the red algal connection to green plants revisited

Cited by 11 publications

References 66 publications

Evolutionary and Regulatory Pattern Analysis of Soybean Ca2+ ATPases for Abiotic Stress Tolerance

Evolutionary and Regulatory Pattern Analysis of Soybean Ca2+ ATPases for Abiotic Stress Tolerance

Energizing the plasmalemma of marine photosynthetic organisms: the role of primary active transport

Predicted AS3MT Proteins Methylate Arsenic and Support Two Major Phylogenetic AS3MT Groups

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