Evolution of some variable-number tandem repeat loci among a group of Beijing strains of Mycobacterium tuberculosis

Billamas, Pamaree; Smittipat, Nat; Juthayothin, Tada; Thong-On, Arunee; Yamada, Norio; Yanai, Hideki; Palittapongarnpim, Prasit

doi:10.1016/j.tube.2007.08.005

Cited by 3 publications

(2 citation statements)

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“…There is an increasing amount of evidence regarding the importance of subtle regional differences in Beijing genotypes due to evolutionary aspects, for example, Billamas et al reported that in Thailand, the variations of one or more MIRU-VNTR loci in Beijing isolates of highly similar IS 6110 -RFLP patterns could imply the occurrence of evolutionary MIRU-VNTR loci among genetically homogeneous M. tuberculosis clinical isolates [26]. Previous studies demonstrated that M. tuberculosis population structure varied geographically in China, mainly from north to south, with southern China showing a relatively smaller proportion of Beijing isolates with a greater distribution frequency of non-Beijing types [27, 28].…”

Section: Introductionmentioning

confidence: 99%

Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-ResistantMycobacterium tuberculosisClinical Isolates from Sichuan in China

Zheng

Zhao

Zhu

et al. 2014

BioMed Research International

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Genotypes of Mycobacterium tuberculosis complex (MTBC) vary with the geographic origin of the patients and can affect tuberculosis (TB) transmission. This study was aimed to further differentiate spoligotype-defined clusters of drug-resistant MTBC clinical isolates split in Beijing (n = 190) versus non-Beijing isolates (n = 84) from Sichuan region, the second high-burden province in China, by IS6110-restriction fragment length polymorphism (RFLP) and 24-locus MIRU-VNTRs. Among 274 spoligotyped isolates, the clustering ratio of Beijing family was 5.3% by 24-locus MIRU-VNTRs versus 2.1% by IS6110-RFLP, while none of the non-Beijing isolates were clustered by 24-locus MIRU-VNTRs versus 9.5% by IS6110-RFLP. Hence, neither the 24-locus MIRU-VNTR was sufficient enough to fully discriminate the Beijing family, nor the IS6110-RFLP for the non-Beijing isolates. A region adjusted scheme combining 12 highly discriminatory VNTR loci with IS6110-RFLP was a better alternative for typing Beijing strains in Sichuan than 24-locus MIRU-VNTRs alone. IS6110-RFLP was for the first time introduced to systematically genotype MTBC in Sichuan and we conclude that the region-adjusted scheme of 12 highly discriminative VNTRs might be a suitable alternative to 24-locus MIRU-VNTR scheme for non-Beijing strains, while the clusters of the Beijing isolates should be further subtyped using IS6110-RFLP for optimal discrimination.

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Section: Introductionmentioning

confidence: 99%

Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-ResistantMycobacterium tuberculosisClinical Isolates from Sichuan in China

Zheng

Zhao

Zhu

et al. 2014

BioMed Research International

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show abstract

“…Tandem repeats have also been found in many bacterial species, including Escherichia coli , Salmonella , Shigella , Vibrio cholerae , Leptospira , and non-tuberculous mycobacteria. The usefulness of such repeats for epidemiological studies is being evaluated [50].…”

Section: Bioinformatics Development and Data Utilizationmentioning

confidence: 99%

Outlook on Thailand's Genomics and Computational Biology Research and Development

Tongsima

Palittapongarnpim

2008

PLoS Comput Biol

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Application of next-generation sequencing for genetic and phenotypic studies of bacteria

Palittapongarnpim

2024

Phylogenomics

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Evolution of some variable-number tandem repeat loci among a group of Beijing strains of Mycobacterium tuberculosis

Cited by 3 publications

References 13 publications

Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-ResistantMycobacterium tuberculosisClinical Isolates from Sichuan in China

Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-ResistantMycobacterium tuberculosisClinical Isolates from Sichuan in China

Outlook on Thailand's Genomics and Computational Biology Research and Development

Application of next-generation sequencing for genetic and phenotypic studies of bacteria

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