Evolution of the Portulaca oleracea L. aggregate in Egypt on molecular and phenotypic levels revealed by morphology, inter-simple sequence repeat (ISSR) and 18S rDNA gene sequence markers

El-Bakatoushi, Ranya; Alframawy, Asmaa Mohamed; Samer, Mona; El-Sadek, Laila; Botros, William A.

doi:10.1016/j.flora.2013.07.008

Cited by 21 publications

(5 citation statements)

References 31 publications

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“…There was no significant relation between morphological characters and the molecular marker, which agreed with the reports of Shao et al (2010) and El-Bakatoushi et al (2013). Probably because different sites between them were taken, many morphological characteristics were affected by the physiological status of individual plants and surrounding environment, and there was no link between marker and the gene controlling the morphological character (Shao et al 2010;Solouki et al 2008).…”

Section: Discussionsupporting

confidence: 82%

Evaluation of genetic diversity within asparagus germplasm based on morphological traits and ISSR markers

Chen

Guo

Jian

et al. 2020

Physiol Mol Biol Plants

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Asparagus officinalis L. is a dioecious perennial plant globally known for its fine flavor and high nutritional value. An evaluation of genetic diversity in 46 asparagus accessions was carried out based on morphological and inter-simple sequence repeat (ISSR) markers. The result show that the coefficient of variation for 20 morphological characteristics is between 12.45 and 62.22%. Factor analysis revealed that nine factors explained 83.37% of the total variance. At Euclidean distance of 135.7, 46 accessions were divided into two clusters. Genetic similarity coefficient (GSC) based on ISSR data ranged from 0.60 to 0.97, suggesting a relatively abundant genetic base. Furthermore, the 46 asparagus accessions could also be grouped into three major clusters at a GSC of 0.74. And there is no significant relation between the two marker systems using the Mantel test. Clustering based on morphological traits compared with that based on ISSR data was not consistent, however, some common groupings were observed between two dendrograms. Therefore the results elucidated asparagus germplasm genetic background and determined hybrid parents, which will facilitate optimal application of asparagus germplasm resources and provide additional data for genetic improvement.

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Section: Discussionsupporting

confidence: 82%

Evaluation of genetic diversity within asparagus germplasm based on morphological traits and ISSR markers

Chen

Guo

Jian

et al. 2020

Physiol Mol Biol Plants

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“…In the present study, ISSR, ITS-RFLP, ITS-SSCP and SSR markers were used to assess the genetic diversity in P. harmala . Inter Simple Sequence Repeats (ISSR) markers have proved their effectiveness for population genetic studies and for studying taxonomic relationships at or below the species level [7], [14], [16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity in wild populations of Peganum harmala L., a medicinal plant

El-Bakatoushi

Ahmed

2018

Journal of Genetic Engineering and Biotechnology

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Peganum harmala L. is a perennial herbaceous plant and can be a future drug due to its wide medicinal purposes. Despite its economic importance, the molecular genetics of P. harmal have not yet been studied in detail. Genetic diversity of 12 P. harmala genotypes were investigated by using Inter-Simple Sequence Repeats (ISSR), PCR-RFLP of rDNA-ITS, PCR-SSCP of rDNA-ITS and Simple Sequence Repeat (SSR) markers. The level of polymorphism revealed by ITS-SSCP is the lowest, followed by ITS-RFLP then ISSR and the highest polymorphism level was reported for SSR marker. The AMOVA analysis implied that most of the variation occurred within the Populations. A value of inbreeding coefficient Fis estimated by the three co-dominant markers was nearly equal and offer an indication of the partial out-crossing reproductive system of P. harmala. Principal Coordinate Analysis (PCOA) plot revealed a clear pattern of clustering based on the locations of collected plants which coincide with the isolation by distance. The study revealed that ITS-SSCP and ISSR markers respectively were more informative than the other used markers in the assessment of genetic diversity of P. harmala. The results reflect the great diversity of P. harmala and data obtained from this study can be used for future collecting missions.

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“…A similar and even more rigorous argument was made by Legrand (), who stated that seed surface characters in the P. oleracea complex were not dependable for taxonomic classification. This concept is backed by a recent DNA‐based population genetic study, which attributed merely 6.41% of the total genetic variation to differences among species (four species studied) as opposed to 31.71% variation among and 61.88% within populations, respectively (El‐Bakatoushi et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Cross pollination by insect visitation is rare (Matthews et al ., ) and wind pollination seems not to take part due to sticky pollen. However, the relative frequency of autogamy and allogamy was studied exemplarily only for the P. oleracea complex (Miyanishi & Cavers, ; see also El‐Bakatoushi et al ., ) and the evolutionary significance of the breeding system is still poorly understood in P. oleracea .…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric, chromosomal and morphometric analyses challenge current taxonomic concepts in thePortulaca oleraceacomplex (Portulacaeae, Caryophyllales)

2015

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Portulaca oleracea is a noxious annual weed of worldwide distribution in temperate to tropical climates. Its taxonomy has been treated in contradictory ways in the past. Various microspecies have been described, lumped into a single species by other authors. We re-examined the importance of seed size and ploidy variation, previously applied as the most important taxonomic characters, for systematic classification based on accessions from Europe, Asia, Africa and South America using flow cytometry, chromosome counting and morphometry. Sixteen microspecies and six transitional forms, covering the ploidy and seed character variation, proposed for the complex, were studied from 178 populations. Portulaca grandiflora was included as a reference species from outside the complex. DNA hyper-pentaploidy or hexaploidy were inferred for the majority of accessions which exhibited the full range of seed size. It is recommended that the only species of lower ploidy (either diploid based on x = 12 or tetraploid based on x = 12) encountered, P. nicaraguensis, should be separated from the P. oleracea complex as it deviates in base chromosome number and monoploid genome size. The frequency distribution of seed size was continuous and unimodal within the wild taxa of the complex and in pairs of taxa defined by testa sculpture. Seed size of DNA hexaploids was slightly negatively correlated with sample/standard fluorescence intensities. Our results conflict with the current microspecies concept. Possible reasons underlying the discrepancy are discussed and strategies for future systematic research are suggested.

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Evolution of the Portulaca oleracea L. aggregate in Egypt on molecular and phenotypic levels revealed by morphology, inter-simple sequence repeat (ISSR) and 18S rDNA gene sequence markers

Cited by 21 publications

References 31 publications

Evaluation of genetic diversity within asparagus germplasm based on morphological traits and ISSR markers

Evaluation of genetic diversity within asparagus germplasm based on morphological traits and ISSR markers

Evaluation of genetic diversity in wild populations of Peganum harmala L., a medicinal plant

Flow cytometric, chromosomal and morphometric analyses challenge current taxonomic concepts in thePortulaca oleraceacomplex (Portulacaeae, Caryophyllales)

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