Evolutionary biology of prolactins and somatotropins II. Electrophoretic comparison of tetrapod somatotropins

Nicoll, Charles S.; Licht, Paul

doi:10.1016/0016-6480(71)90184-5

Cited by 75 publications

(19 citation statements)

References 35 publications

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“…The running gel was made with acrylamide (75 g/1), pH 8-9, and a tris-glycine buffer system, pH 8-6, was used. The fastest migrating band was determined as PRL (Nicoll and Nichols, 1971;Yamamoto et al, 1977). The stained PRL band was estimated by densitometric measurement using a recording and integrating densitometer (OZMOR R-802) with 4-mm slit and 570 ^m filter.…”

Section: Methodsmentioning

confidence: 99%

Effects of ageing and gonadal steroid hormones on prolagtin concentration in the anterior pituitary of the chicken

Kono

Ichinoe

Nakajo

1980

British Poultry Science

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1. Effects of ageing and gonadal steroid hormones on pituitary prolactin (PRL) concentration in the chicken were investigated. 2. No difference was found in the qualitative electrophoretic patterns of the anterior pituitary homogenates at different ages and between the sexes. 3. Pituitary PRL concentrations in males were generally higher than in females. 4. Pituitary PRL concentrations in castrated immature cockerels were higher than in intact birds, while PRL concentration was depressed by the injection of testosterone propionate or oestradiol benzoate. 5. Prolactin concentration in intact immature cockerels was decreased by daily injection of testosterone or oestradiol for 3 weeks but not by injection of the hormones for 1 week.

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Section: Methodsmentioning

confidence: 99%

Effects of ageing and gonadal steroid hormones on prolagtin concentration in the anterior pituitary of the chicken

Kono

Ichinoe

Nakajo

1980

British Poultry Science

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“…Our data show that under different electrophor etic conditions, the PRL band of the hamster standard, as well as those of the samples from intact and blinded ham sters, run identically. While it is possible that a structurally related pituitary protein such as a growth hormone (GH) could contaminate the PRL band, this seems rather unlikely since GH in both rat and hamster pituitary homogenates runs far behind PRL during polyacrylamide disc gel elec trophoresis [20,21]. Furthermore, the results of the present study confirm the work of others showing that both rat and mouse PRLs run behind hamster PRL during electrophore sis [10,20,21]; thus, this technique is capable of separating very closely related molecules.…”

Section: Discussionmentioning

confidence: 99%

Neuroendocrine Effects of Light Deprivation and Pinealectomy in vivo on the Time Course of Changes in Prolactin Cell Activity in vitro

Km¹,

De²

1987

Neuroendocrinology

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The purpose of this investigation was to elucidate the differences in the nature and time course of changes in in vitro prolactin (PRL) cell activity between blinded and blind/pinealectomized female hamsters. Adult female golden hamsters were either left intact, blinded and sham-pinealectomized (Blind/Sham) or blinded and pinealectomized (Blind/ Pinx). Twelve weeks after surgeries, animals were killed by decapitation and randomized hempituitaries were incubated for a total of either 30, 60, 90, 120, 180 or 240 min. PRL release in vivo, as estimated by monitoring serum titers of immunoreactive PRL (IR-PRL), was markedly reduced in Blind/Sham animals; pinealectomy completely prevented this depression. PRL storage was assessed by measuring total levels (i.e., medium + pituitaries) of IR-PRL in vitro. Total, pituitary and media values of IR-PRL were all significantly depressed in Blind/Sham females. Pinealectomy of blinded animals almost completely prevented these reductions. PRL synthesis, as evaluated by measuring the amount of ³H-leucine incorporated into PRL in vitro, was profoundly reduced in Blind/Sham females. Surprisingly, pinealectomy failed to prevent the blinding-induced decrease in PRL production. From these data, we conclude that in the light-deprived female hamster the pineal gland inhibits PRL storage and release, while the depression in PRL synthesis may be independent of a pineal influence.

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“…It has been applied to the separation of hormones from pituitary homogenates from rats (Jones et al 1965;Kragt and Meites 1966;Hodges and McShan 1970), mice , eels (Knight et al 1970), and flounders (Chadwick 1970). Recently, electrophoresis has been used to demonstrate the existence of prolactin and growth hormone as separate entities in a variety of vertebrate species from fishes to mammals (Nicoll and Nichols 1971;. The quantity of prolactin in pituitaries or in culture media has been determined by measuring the optical density of Wesent address: Hormone Research Laboratory, University of California, San Francisco, California 94143. the stained bands after electrophoretic separation Nicoll et al 1969;Ball and Ingleton 1973).…”

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confidence: 97%

Disc-electrophoretic identification of prolactin in the cichlid teleosts Tilapia and Cichlasoma and densitometric measurement of its concentration in Tilapia pituitaries during salinity transfer experiments

Clarke¹

1973

Can. J. Zool.

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CLARKE, W. C. 1973. Disc-electrophoretic identification of prolactin in the cichlid teleosts T i l a p i a .~d Cichlasoma and densitometric measurement of its concentration in Tilapia pituitaries dunng salvllty transfer experiments. Can. J. Zool. 51: 687-695. The prolactin band was identified after disc electrophoresis of pituitaries from Tilapia mossambica and Cichlasoma labiaturn. Electrophoresis coupled with a densitometric procedure was validated as a means of measuring the prolactin content of Tilapia pituitaries during s a h t y transfer experiments. Transfer of T. mossambica from seawater or 33% seawater to fresh water was followed by a sharp decline in both p s m a sodium and pituitary prolactin levels. One week after transfer, there was a recovery of sodium eve!s to normal, although pituitary prolactin concentrations had not yet reached the level found in fully acclimated freshwater fish. Pituitary prolactin levels in T. grahami were reduced by more than 50% on the day after transfer from Lake Magadi (alkaline-saline) water to fresh water. No such depleuon of pituitary prolactin followed a transfer from 100% seawater or from fresh water to 33% seawater, m&-catmg that thls response 1s specifically linked to the secretion of prolactin for osmoregulafion m fresh water. The molecular weight of Tilapia prolactin was estimated at 17 000 by means of electrophoresis in sodium dodecyl sulfate. CLARKE, W. C. 1973. Disc-electrophoretic identification of prolactin in the cichlid te!eosts Tilapia.apd Cichlasoma and densitometric measurement of its concentration in Tilapia pitutanes d w g s-ty transfer experiments. Can. J. Zool. 51: 687-695. Les hypophyses de Tilapia mossambica et de Cichlusoma Inbiafum ont ftC soumises k une 4Iectrophorhe verticale ( b c electro horesis) et on a rCussi B identifier le disque & prdactine. L'elecboph& associk B une technique de Jnsitomktrie constitue une mkthade ualable pour m s u n r h motenu de prolactine des hypophyses de Tila ia, au cours d'ex riences dc bansfert 3 dzerentes salinit6s. ix passage de T. mossambica de l'eau i e mer ou d'une so P' ution d'r~u de mer 33:5 h I'eau douce cause une diminutim marquee du sodium dans le plasma et des concentrations de prolactine dans l'hypophyse. Une semme pius rard, Ia concentration de sodium est redevenut normale alors que la concentratron de prolact~ne n'a pas encore atteint le niveau normal d'un pisson entikrernent acclimatd B I'eau douce.Chez des T.~ro/romi eansferes de I'cau ( a l c a h~a l i n e )du lac Magadi b de L'enu douce, on eurcgkistre, le lendemain, une &-duction de plus de 50% de Ia concentration dc prolactine de I'hypophpe. Par contrc, cette d~rninu~~on ne se produit pas aprts le transfert d'eau dc mes 100';; ou d'eau douce: a de I'eau de mer 33%; ceci indique ue la rkact~on est reli.6~ spkifi uement ti la sicrdtion de prolacme pour lin d'osrnorCguIaiion en eau !Duct. Le poids moibulaire de ?a proih~tioe da Tiloplo est estimC h 17 003 par ~lcctrophoikse dam le suIfate de sodium et de dodkylc.rraduit par le journal]

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Evolutionary biology of prolactins and somatotropins II. Electrophoretic comparison of tetrapod somatotropins

Cited by 75 publications

References 35 publications

Effects of ageing and gonadal steroid hormones on prolagtin concentration in the anterior pituitary of the chicken

Effects of ageing and gonadal steroid hormones on prolagtin concentration in the anterior pituitary of the chicken

Neuroendocrine Effects of Light Deprivation and Pinealectomy in vivo on the Time Course of Changes in Prolactin Cell Activity in vitro

Disc-electrophoretic identification of prolactin in the cichlid teleosts Tilapia and Cichlasoma and densitometric measurement of its concentration in Tilapia pituitaries during salinity transfer experiments

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