Evolutionary conservation and divergence of Vasa , Dazl and Nanos1 during embryogenesis and gametogenesis in dark sleeper ( Odontobutis potamophila )

Zhu, Wenxu; Wang, Tao; Zhao, Cheng; Wang, Dan; Zhang, Xinyu; Zhang, Hongyan; Chi, Meili; Yin, Shaowu; Jia, Yongyi

doi:10.1016/j.gene.2018.06.016

Cited by 10 publications

(6 citation statements)

References 60 publications

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“…Additionally, the genes could have been methylated because they have no role in certain stages [ 85 ]. In our study, the increase in vasa expression at 430 d°C, which corresponds to the yolk sac larval stage in S. salar , is in contrast with the results of the other teleost, in which low expression was detected [ 86 , 87 , 88 ]. In situ hybridization and immunohistochemistry detected the presence of vasa at a higher level in hatched larvae, which corroborates with the present results [ 79 ].…”

Section: Discussioncontrasting

confidence: 94%

Insights into Early Ontogenesis of Salmo salar: RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

Bhat

Dubiel

Rodrı́guez³

et al. 2023

Animals

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The challenge in extracting high-quality RNA impedes the investigation of the transcriptome of developing salmonid embryos. Furthermore, the mRNA expression pattern of important PGC and SD genes during the initial embryonic development of Salmo salar is yet to be studied. So, in the present study, we aimed to isolate high-quality RNA from eggs and developing embryos to check vasa, dnd1, nanos3a, sdf1, gsdf, amh, cyp19a, dmrt1 and foxl2 expression by qPCR. Additionally, four HKGs (GAPDH, UB2L3, eEf1a and β-actin) were validated to select the best internal control for qPCR. High-quality RNA was extracted, which was confirmed by spectrophotometer, agarose gel electrophoresis and Agilent TapeStation analysis. UB2L3 was chosen as a reference gene because it exhibited lower intra- and inter-sample variation. vasa transcripts were expressed in all the developmental stages, while dnd1 was expressed only up to 40 d°C. Nanos3a was expressed in later stages and remained at its peak for a shorter period, while sdf1 showed an irregular pattern of mRNA expression. The mRNA expression levels of SD genes were observed to be upregulated during the later stages of development, prior to hatching. This study presents a straightforward methodology for isolating high-quality RNA from salmon eggs, and the resulting transcript profiles of significant PGC and SD genes in S. salar could aid in improving our comprehension of reproductive development in this commercially important species.

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Section: Discussioncontrasting

confidence: 94%

Insights into Early Ontogenesis of Salmo salar: RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

Bhat

Dubiel

Rodrı́guez³

et al. 2023

Animals

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“…13 The expression of DDX4 has been reported in undifferentiated seminiferous tubule cells (spermatogonia and spermatocytes), while no DDX4 expression was detected in the sperm. 36 In the present study, immunofluorescence results indicated that the expression of DDX4 was positive in spermatogonia and spermatocytes, which is consistent with previous studies. Further analysis showed that the level of DDX4 was higher in mice fed a HFD than in those fed a ND.…”

Section: Discussionsupporting

confidence: 93%

The time-dependent adverse effects of a high-fat diet on sperm parameters

Qi¹,

Guan²,

Zhang³

et al. 2023

Adv Clin Exp Med

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Background. Studies indicate a relationship between a high-fat diet (HFD) and sperm quality. However, the time-dependent adverse effects of a HFD on sperm parameters and the underlying mechanisms remain unclear.Objectives. The present study was designed to determine the effects of a HFD on sperm quality at various time points in order to assess whether a HFD causes cumulative damage to sperm. Materials and methods.Male C57BL/6 mice were fed a normal diet (the ND group) or a HFD (the HFD group) for 16, 30 or 42 weeks (n = 6 for each group). Body weight, lipid profile, sperm parameters, testicular morphology, and testicular oxidative stress levels were evaluated alongside the proliferation, DNA damage and rate of germ cell apoptosis.Results. Sperm quality was reduced in HFD-fed animals in a time-dependent manner, which was demonstrated by a decline in sperm density, motility and progressive motility. Further analysis showed a progressive deterioration of the testicular histoarchitecture of HFD-fed mice, which was accompanied by a decrease in DEAD-box helicase 4 (DDX4) expression and superoxide dismutase (SOD) levels, increased malondialdehyde (MDA) levels and gamma-H2A histone family member X (γ-H2AX) expression, and increased apoptosis of germ cells.Conclusions. These findings demonstrate that a HFD exerted adverse effects on sperm quality, and the deteriorating effect was progressive with long-term feeding. The inhibited proliferation and apoptosis of germ cells, and the increased oxidative stress levels and DNA damage may be the underlying mechanisms.

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“…During testicular development, Rcvasa mRNA signal was mainly concentrated in the periphery of spermatogonia, primary spermatocytes, and secondary spermatocytes, and it was found to be weak in spermatids and spermatozoa. Similar results were reported in other fish [34,35]. The localization of Rcvasa mRNA during spermatogenesis suggested that this protein might play a major role in the transformation of spermatogonia into primary spermatocytes, but not in the development and maturation of spermatids.…”

Section: Discussionsupporting

confidence: 88%

Cloning and Expression Profiling of the Gene vasa during First Annual Gonadal Development of Cobia (Rachycentron canadum)

Qian

Kuang

Chen

et al. 2022

Fishes

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The vasa gene is essential for germ cell development and gametogenesis both in vertebrates and in invertebrates. In the present study, vasa (Rcvasa) cDNA was cloned from cobia (Rachycentron canadum) using the RACE amplification method. We found that the full-length cDNA sequence of Rcvasa comprises 2571 bp, containing a 5′-UTR of 145 bp, a 3′-UTR of 341 bp, and an open reading frame (ORF) of 2085 bp, encoding a protein of 694 aa. The deduced amino acid sequence contains 8 conserved motifs of the DEAD-box protein family, 7 RGG repeats, and 10 RG repeats in the N-terminal region. Comparisons of the deduced amino acid sequence with those of other teleosts revealed the highest percentage identity (86.0%) with Seriola quinqueradiata. By using semiquantitative RT-PCR, Rcvasa appeared to be specifically expressed in the testis and ovary, among 13 tissues analyzed. In addition, annual changes in Rcvasa expression levels were examined in the gonads by quantitative real-time PCR (qRT-PCR). The expression of Rcvasa in the testis first increased significantly at 120 dph (stage II–III), then stabilized as the testis developed from 185 dph (stage III) to 360 dph (stage V). During the development of the ovary (stage I to II), the expression of Rcvasa first increased and reached the highest level at 210 dph (stage II), then decreased. Furthermore, the results of chromogenic in situ hybridization (CISH) revealed that Rcvasa mRNA was mainly expressed in germ cells and barely detected in somatic cells. In the testis, Rcvasa mRNA signal was concentrated in the periphery of spermatogonia, primary spermatocytes, and secondary spermatocytes and was significantly weaker in spermatids and spermatozoa. In the ovary, Rcvasa mRNA signal was uniformly distributed in the perinuclear cytoplasm and was intense in early primary oocytes (stage I and II). These findings could provide a reference for understanding the regulatory mechanisms of vasa expression during the development of germ cells in cobia.

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Evolutionary conservation and divergence of Vasa , Dazl and Nanos1 during embryogenesis and gametogenesis in dark sleeper ( Odontobutis potamophila )

Cited by 10 publications

References 60 publications

Insights into Early Ontogenesis of Salmo salar: RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

Insights into Early Ontogenesis of Salmo salar: RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

The time-dependent adverse effects of a high-fat diet on sperm parameters

Cloning and Expression Profiling of the Gene vasa during First Annual Gonadal Development of Cobia (Rachycentron canadum)

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