Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling

Lori, Martina; Verk, Marcel C. Van; Hander, Tim; Schatowitz, Hendrik; Klauser, Dominik; Flury, Pascale; Gehring, Chris; Boller, Thomas; Bartels, Sebastian

doi:10.1093/jxb/erv236

Cited by 100 publications

(122 citation statements)

References 38 publications

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“…As shown in Table 2 , the alkalinization effect of AtPEP from Arabidopsis is significantly stronger in Arabidopsis suspension cells, while systemin from potato stimulated alkalinization only in potato suspension cells. These results are consistent with previous reports showing species specificity in the peptidic DAMPs (Lori et al, 2015). …”

Section: Resultssupporting

confidence: 94%

Extracellular Alkalinization as a Defense Response in Potato Cells

et al. 2017

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A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a defense response in potatoes. Using potato suspension cell cultures, we observed an alkalinization response against various pathogen- and plant-derived elicitors in a dose- and time-dependent manner. We also assessed the defense response against a variety of potato pathogens, such as protists (Phytophthora infestans and Spongospora subterranea) and fungi (Verticillium dahliae and Colletotrichum coccodes). Our results show that extracellular pH increases within 30 min in proportion to the number of pathogen spores added. Consistently with the alkalinization effect, the higher transcription level of several defense-related genes and production of reactive oxygen species was observed. Our results demonstrate that the alkalinization response is an effective marker to study early stages of defense response in potatoes.

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Section: Resultssupporting

confidence: 94%

Extracellular Alkalinization as a Defense Response in Potato Cells

et al. 2017

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“…PmPROPEP1 corresponds to PmPROPEP5, described in Lori et al . (). (c) Depiction of the consensus sequences of aligned Rosaceae‐specific Pep sequences using the WebLogo tool (Crooks et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…Most plant species contain one or two PEPRs, and conservation of their Pep‐recognition LRR domain is lower than that of the catalytic kinase domain (Flury et al ., ; Huffaker et al ., ; Lori et al ., ; Yamaguchi et al ., ). Peps from a given plant species can only be perceived by plants from the same family (Huffaker et al ., ; Lori et al ., ), even though downstream pathways leading to PTI seem to be highly conserved among species (Lori et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Novel Rosaceae plant elicitor peptides as sustainable tools to control Xanthomonas arboricola pv. pruni in Prunus spp.

Ruiz

Nadal

Seguí

et al. 2017

Molecular Plant Pathology

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Fruit crops are regarded as important health promoters and constitute a major part of global agricultural production, and Rosaceae species are of high economic impact. Their culture is threatened by bacterial diseases, whose control is based on preventative treatments using compounds of limited efficacy and negative environmental impact. One of the most economically relevant examples is the pathogen Xanthomonas arboricola pv. pruni (Xap) affecting Prunus spp. The plant immune response against pathogens can be triggered and amplified by plant elicitor peptides (Peps), perceived by specific receptors (PEPRs). Although they have been described in various angiosperms, scarce information is available on Rosaceae species. Here, we identified the Pep precursor (PROPEP), Pep and PEPR orthologues of 10 Rosaceae species and confirmed the presence of the Pep/PEPR system in this family. We showed the perception and elicitor activity of Rosaceae Peps using the Prunus-Xap pathosystem as proof-of-concept. Treatment with nanomolar doses of Peps induced the corresponding PROPEP and a set of defence-related genes in Prunus leaves, and enhanced resistance against Xap. Peps from the same species had the highest efficiencies. Rosaceae Peps could potentially be used to develop natural, targeted and environmentally friendly strategies to enhance the resistance of Prunus species against biotic attackers.

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“…Recently, analysis of the crystal structure of the At-PEP1-PEPR1 complex revealed that At-PEP1 adopts a fully extended conformation and binds to the inner surface of the superhelical extracellular LRR domain of PEPR1 (Tang et al, 2015). The LRR domains of PEPRs coevolved with the PEPs, leading to distinct PEP motifs in each plant family and interfamily incompatibility of recognition, in contrast to the highly conserved downstream signaling (Lori et al, 2015). In general, the PEP immune signaling pathway is associated with an early cytosolic Ca 2+ influx that activates protein kinases, reactive oxygen species, and nitrogen oxide .…”

Section: Non-cysteine-rich Peptides Without Specific Posttranslationamentioning

confidence: 99%

The Plant Peptidome: An Expanding Repertoire of Structural Features and Biological Functions

et al. 2015

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Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling

Abstract: HighlightPlant elicitor peptides (Peps) co-evolved with their receptors, resulting in interfamily incompatibility of Pep recognition. In contrast, operation of defence pathways by Pep receptors is conserved within the flowering plants.

Cited by 100 publications

References 38 publications

Extracellular Alkalinization as a Defense Response in Potato Cells

Extracellular Alkalinization as a Defense Response in Potato Cells

Novel Rosaceae plant elicitor peptides as sustainable tools to control Xanthomonas arboricola pv. pruni in Prunus spp.

The Plant Peptidome: An Expanding Repertoire of Structural Features and Biological Functions

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