Evolutionary history of glucose-6-phosphatase encoding genes in vertebrate lineages: towards a better understanding of the functions of multiple duplicates

Marandel, Lucie; Panserat, Stéphane; Plagnes-Juan, Élisabeth; Arbenoits, Eva; Soengas, José L.; Bobe, Julien

doi:10.1186/s12864-017-3727-1

Cited by 17 publications

(9 citation statements)

References 50 publications

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“…treatment resulted in an increased activity of Gsase, as well as an increased mRNA abundance of GK, GLUT2, GSase and Kir6.x‐like. Furthermore, the increase observed in the values of G6Pase is in agreement with the effect observed in the same brain region after feeding rainbow trout with a carbohydrate‐enriched diet . Strikingly, GK activity did not follow changes indicated by its mRNA abundance.…”

Section: Discussionsupporting

confidence: 86%

“…treatment with glucose enhanced CART mRNA abundance in the telencephalon of catfish and zebrafish (ie, comparable responses to those previously described in other glucosensing areas in fish, such as the hypothalamus and hindbrain) . Second, the mRNA abundance of g6pcb2b , a gene expressing the enzyme glucose 6‐phosphatase (G6Pase) involved in glucose metabolism, increased in the telencephalon of rainbow trout fed with a carbohydrate‐enriched diet . The function of these hypothetical glucosensing mechanisms in the telencephalon might relate to metabolic control of food intake via either homeostatic or hedonic (reward) pathways.…”

Section: Introductionsupporting

confidence: 67%

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Glucosensing capacity of rainbow trout telencephalon

Otero-Rodiño

Rocha

Álvarez-Otero

et al. 2018

J Neuroendocrinology

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To assess the hypothesis of glucosensing systems present in fish telencephalon, we first demonstrated in rainbow trout, by in situ hybridisation, the presence of glucokinase (GK). Then, we assessed the response of glucosensing markers in rainbow trout telencephalon 6 hours after i.c.v. treatment with glucose or 2-deoxyglucose (inducing glucoprivation). We evaluated the response of parameters related to the mechanisms dependent on GK, liver X receptor (LXR), mitochondrial activity, sweet taste receptor and sodium-glucose linked transporter 1 (SGLT-1). We also assessed mRNA abundance of neuropeptides involved in the metabolic control of food intake (agouti-related protein, neuropeptide Y, pro-opiomelanocortin, and cocaine- and amphetamine-related transcript), as well as the abundance and phosphorylation status of proteins possibly involved in linking glucosensing with neuropeptide expression, such as protein kinase B (AkT), AMP-activated protein kinase (AMPK), mechanistic target of rapamycin and cAMP response element-binding protein (CREB). The responses obtained support the presence in the telencephalon of a glucosensing mechanism based on GK and maybe one based on LXR, although they do not support the presence of mechanisms dependent on mitochondrial activity and SGLT-1. The mechanism based on sweet taste receptor responded to glucose but in a converse way to that characterised previously in the hypothalamus. In general, systems responded only to glucose but not to glucoprivation. Neuropeptides did not respond to glucose or glucoprivation. By contrast, the presence of glucose activates Akt and inhibits AMPK, CREB and forkhead box01. This is the first study in any vertebrate species in which the response to glucose of putative glucosensing mechanisms is demonstrated in the telencephalon. Their role might relate to processes other than homeostatic control of food intake, such as the hedonic and reward system.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionsupporting

confidence: 67%

Glucosensing capacity of rainbow trout telencephalon

Otero-Rodiño

Rocha

Álvarez-Otero

et al. 2018

J Neuroendocrinology

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“…When more than 20% of FM is substituted by digestible carbohydrates in the diet, they typically display a decrease in growth and a persistent postprandial hyperglycemia. Different hypotheses have been proposed to explain this phenomena (Kamalam et al, 2017) and among them, is the lack of inhibition in the last step of the hepatic gluconeogenesis (Marandel et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Exploring the Impact of a Low-Protein High-Carbohydrate Diet in Mature Broodstock of a Glucose-Intolerant Teleost, the Rainbow Trout

Callet

Larroquet

et al. 2020

Front. Physiol.

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Callet et al. Carbohydrate Utilization in Trout Broodstock shown that broodstock are able to use a diet containing digestible carbohydrates as high as 35% and can then grow and reproduce normally over an entire reproductive cycle for females and at least at the beginning of the cycle for males. These results are highly promising and suggest that dietary carbohydrates can at least partially replace proteins in broodstock aquafeed.

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“…In addition, although recent studies suggested that fish, especially carnivorous fish, lacked the ability to regulate gluconeogenesis (45)(46)(47)(48), the expression of key genes involved in gluconeogenesis in mandarin fish after IP of leptins were decreased as in mammals. In rat, leptin was reported to reduce hepatic glucose production by decreasing the synthesis of the key enzyme of gluconeogenesis (49).…”

Section: Discussionmentioning

confidence: 99%

Differential Roles of Two Leptin Gene Paralogues on Food Intake and Hepatic Metabolism Regulation in Mandarin Fish

et al. 2020

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Leptin affects food intake regulation and energy homeostasis in mammals, as opposed to mammals who have a single leptin gene, fish have duplicated leptin gene paralogues. Until now, most functional studies on fish focused on the first reported paralogue without much explanation on specific gene paralogue. This study successfully expressed two homologous recombinant mandarin fish leptin genes (LepA and LepB) for the first time. To explore the differential roles of these two gene paralogues involved in food intake and energy homeostasis, mandarin fish were treated with homologous recombinant LepA and LepB proteins by acute IP administration. The results showed that LepB inhibited the food intake of mandarin fish after acute IP administration through modifying the expressions of hypothalamic orexigenic genes, while LepA had no significant effect on its food intake. In addition, LepB administration decreased the hepatic glycogen level through regulating the gene expressions of glycogen synthase and glycogen phosphorylase in mandarin fish until 4 d, while LepA did not change the hepatic glycogen level as it failed to change the expressions of these regulatory genes. Moreover, LepA and LepB downregulated the expressions of key gluconeogenic genes (phosphofructokinase, phosphoenolpyruvate carboxykinase, and glucose-6-phosphatase), indicating both mandarin fish leptins could regulate the rate of glucose production. However, these two gene paralogues presented secondary effects on lipid metabolism as they only enhanced the triglyceride level by modifying the gene expressions of adipose triglyceride lipase or acetyl CoA carboxylase just for 1 d after IP. Therefore, LepB played an important role in food intake and glucose homeostasis regulation, while LepA showed a limited role in gluconeogenesis and lipid metabolism.

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Evolutionary history of glucose-6-phosphatase encoding genes in vertebrate lineages: towards a better understanding of the functions of multiple duplicates

Cited by 17 publications

References 50 publications

Glucosensing capacity of rainbow trout telencephalon

Glucosensing capacity of rainbow trout telencephalon

Exploring the Impact of a Low-Protein High-Carbohydrate Diet in Mature Broodstock of a Glucose-Intolerant Teleost, the Rainbow Trout

Differential Roles of Two Leptin Gene Paralogues on Food Intake and Hepatic Metabolism Regulation in Mandarin Fish

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